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High quality genome sequence and description of Enterobacter mori strain 5-4, isolated from a mixture of formation water and crude-oil.

Zhang F, Su S, Yu G, Zheng B, Shu F, Wang Z, Xiang T, Dong H, Zhang Z, Hou D, She Y - Stand Genomic Sci (2015)

Bottom Line: To date, there is only one E. mori genome has been sequenced and very little knowledge about the mechanism of E. mori adapted to the petroleum reservoir.Here, we report the second E. mori genome sequence and annotation, together with the description of features for this organism.The 4,621,281 bp assembly genome exhibits a G + C content of 56.24% and contains 4,317 protein-coding and 65 RNA genes, including 5 rRNA genes.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, School of Energy Resources, China University of Geosciences, Beijing, China.

ABSTRACT
Enterobacter mori strain 5-4 is a Gram-negative, motile, rod shaped, and facultatively anaerobic bacterium, which was isolated from a mixture of formation water (also known as oil-reservior water) and crude-oil in Karamay oilfield, China. To date, there is only one E. mori genome has been sequenced and very little knowledge about the mechanism of E. mori adapted to the petroleum reservoir. Here, we report the second E. mori genome sequence and annotation, together with the description of features for this organism. The 4,621,281 bp assembly genome exhibits a G + C content of 56.24% and contains 4,317 protein-coding and 65 RNA genes, including 5 rRNA genes.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree highlighting the position of E. mori 5–4 relative to other type strains within the genus Enterobacter. The strains and their corresponding GenBank accession numbers for 16S rRNA genes are shown following the organism names. Bootstrap consensus trees were inferred from 100 replicates, only bootstrap values > 50% were indicated. Xenorhabdus poinarii DSM 4768T was used as anoutgroup. The scale bar, 0.0005 substitutions per nucleotide position.
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Fig2: Phylogenetic tree highlighting the position of E. mori 5–4 relative to other type strains within the genus Enterobacter. The strains and their corresponding GenBank accession numbers for 16S rRNA genes are shown following the organism names. Bootstrap consensus trees were inferred from 100 replicates, only bootstrap values > 50% were indicated. Xenorhabdus poinarii DSM 4768T was used as anoutgroup. The scale bar, 0.0005 substitutions per nucleotide position.

Mentions: A comparative taxonomic analysis was conducted based on the 16S rRNA nucleotide sequence. The representative 16S rRNA nucleotide sequence of Enterobacter mori strain 5–4 was compared against the most recent release of the EzTaxon-e database [26]. CLUSTAL W was used to generate alignments with comparative sequences collected from EzTaxon-e database [27]. The alignments were trimmed and converted to the MEGA 6.06 format before phylogenetic analysis. Phylogenetic inferences were made using Neighbor-joining method based on Tamura-Nei model within the MEGA 6.06 [28]. Phylogenetic tree indicated the taxonomic status of strain 5–2, clearly classified into the same branch with species E. mori type strain LMG 25706T (Figure 2).


High quality genome sequence and description of Enterobacter mori strain 5-4, isolated from a mixture of formation water and crude-oil.

Zhang F, Su S, Yu G, Zheng B, Shu F, Wang Z, Xiang T, Dong H, Zhang Z, Hou D, She Y - Stand Genomic Sci (2015)

Phylogenetic tree highlighting the position of E. mori 5–4 relative to other type strains within the genus Enterobacter. The strains and their corresponding GenBank accession numbers for 16S rRNA genes are shown following the organism names. Bootstrap consensus trees were inferred from 100 replicates, only bootstrap values > 50% were indicated. Xenorhabdus poinarii DSM 4768T was used as anoutgroup. The scale bar, 0.0005 substitutions per nucleotide position.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940761&req=5

Fig2: Phylogenetic tree highlighting the position of E. mori 5–4 relative to other type strains within the genus Enterobacter. The strains and their corresponding GenBank accession numbers for 16S rRNA genes are shown following the organism names. Bootstrap consensus trees were inferred from 100 replicates, only bootstrap values > 50% were indicated. Xenorhabdus poinarii DSM 4768T was used as anoutgroup. The scale bar, 0.0005 substitutions per nucleotide position.
Mentions: A comparative taxonomic analysis was conducted based on the 16S rRNA nucleotide sequence. The representative 16S rRNA nucleotide sequence of Enterobacter mori strain 5–4 was compared against the most recent release of the EzTaxon-e database [26]. CLUSTAL W was used to generate alignments with comparative sequences collected from EzTaxon-e database [27]. The alignments were trimmed and converted to the MEGA 6.06 format before phylogenetic analysis. Phylogenetic inferences were made using Neighbor-joining method based on Tamura-Nei model within the MEGA 6.06 [28]. Phylogenetic tree indicated the taxonomic status of strain 5–2, clearly classified into the same branch with species E. mori type strain LMG 25706T (Figure 2).

Bottom Line: To date, there is only one E. mori genome has been sequenced and very little knowledge about the mechanism of E. mori adapted to the petroleum reservoir.Here, we report the second E. mori genome sequence and annotation, together with the description of features for this organism.The 4,621,281 bp assembly genome exhibits a G + C content of 56.24% and contains 4,317 protein-coding and 65 RNA genes, including 5 rRNA genes.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, School of Energy Resources, China University of Geosciences, Beijing, China.

ABSTRACT
Enterobacter mori strain 5-4 is a Gram-negative, motile, rod shaped, and facultatively anaerobic bacterium, which was isolated from a mixture of formation water (also known as oil-reservior water) and crude-oil in Karamay oilfield, China. To date, there is only one E. mori genome has been sequenced and very little knowledge about the mechanism of E. mori adapted to the petroleum reservoir. Here, we report the second E. mori genome sequence and annotation, together with the description of features for this organism. The 4,621,281 bp assembly genome exhibits a G + C content of 56.24% and contains 4,317 protein-coding and 65 RNA genes, including 5 rRNA genes.

No MeSH data available.


Related in: MedlinePlus