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High quality genome sequence and description of Enterobacter mori strain 5-4, isolated from a mixture of formation water and crude-oil.

Zhang F, Su S, Yu G, Zheng B, Shu F, Wang Z, Xiang T, Dong H, Zhang Z, Hou D, She Y - Stand Genomic Sci (2015)

Bottom Line: To date, there is only one E. mori genome has been sequenced and very little knowledge about the mechanism of E. mori adapted to the petroleum reservoir.Here, we report the second E. mori genome sequence and annotation, together with the description of features for this organism.The 4,621,281 bp assembly genome exhibits a G + C content of 56.24% and contains 4,317 protein-coding and 65 RNA genes, including 5 rRNA genes.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, School of Energy Resources, China University of Geosciences, Beijing, China.

ABSTRACT
Enterobacter mori strain 5-4 is a Gram-negative, motile, rod shaped, and facultatively anaerobic bacterium, which was isolated from a mixture of formation water (also known as oil-reservior water) and crude-oil in Karamay oilfield, China. To date, there is only one E. mori genome has been sequenced and very little knowledge about the mechanism of E. mori adapted to the petroleum reservoir. Here, we report the second E. mori genome sequence and annotation, together with the description of features for this organism. The 4,621,281 bp assembly genome exhibits a G + C content of 56.24% and contains 4,317 protein-coding and 65 RNA genes, including 5 rRNA genes.

No MeSH data available.


Related in: MedlinePlus

Scanning electron micrograph of cells of Enterobacter mori strain 5–4 bar: 2.0 μm.
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Fig1: Scanning electron micrograph of cells of Enterobacter mori strain 5–4 bar: 2.0 μm.

Mentions: A formation water sample was collected from Karamay Oilfield, Xinjiang, China, in 2012. The water sample was preserved at -80°C immediately after collection and sent to the lab. E. mori strain 5–4 was isolated after cultivation on LB agar medium at 37°C. The optimum temperature for growth is 35°C, with a temperature range of 4-45°C (Table 1). Growth occurs under aerobic condition. Grows at pH 5.5-10.0, and optimally at pH 7.0. Cell morphology was examined by using scanning electron microscopy (Quanta 200, FEI Co., USA). Colonies are light yellow, smooth, circular with entire margins, with a diameter ranging 0.3-0.8 μm, and from 0.6 to 1.8 μm long (Figure 1). Themethyl red test is negative. H2S and indole are not produced. Casein and starch are not hydrolysed; gelatin is hydrolysed. Sorbitol, glycerol, tetradecane and hexadecane are utilized as the carbon source, while lactose, rhamnose, glucose, maltose, cellobiose, galactose, raffinose and sucrose are not utilized. Nitrite sodium and ammonium chloride are utilized, while nitrate sodium is not reduced. Antimicrobial susceptibility test showed that this strain is susceptible to ampicillin, tetracycline, erythromycin and gentamicin, and resistant to kanamycin.Table 1


High quality genome sequence and description of Enterobacter mori strain 5-4, isolated from a mixture of formation water and crude-oil.

Zhang F, Su S, Yu G, Zheng B, Shu F, Wang Z, Xiang T, Dong H, Zhang Z, Hou D, She Y - Stand Genomic Sci (2015)

Scanning electron micrograph of cells of Enterobacter mori strain 5–4 bar: 2.0 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940761&req=5

Fig1: Scanning electron micrograph of cells of Enterobacter mori strain 5–4 bar: 2.0 μm.
Mentions: A formation water sample was collected from Karamay Oilfield, Xinjiang, China, in 2012. The water sample was preserved at -80°C immediately after collection and sent to the lab. E. mori strain 5–4 was isolated after cultivation on LB agar medium at 37°C. The optimum temperature for growth is 35°C, with a temperature range of 4-45°C (Table 1). Growth occurs under aerobic condition. Grows at pH 5.5-10.0, and optimally at pH 7.0. Cell morphology was examined by using scanning electron microscopy (Quanta 200, FEI Co., USA). Colonies are light yellow, smooth, circular with entire margins, with a diameter ranging 0.3-0.8 μm, and from 0.6 to 1.8 μm long (Figure 1). Themethyl red test is negative. H2S and indole are not produced. Casein and starch are not hydrolysed; gelatin is hydrolysed. Sorbitol, glycerol, tetradecane and hexadecane are utilized as the carbon source, while lactose, rhamnose, glucose, maltose, cellobiose, galactose, raffinose and sucrose are not utilized. Nitrite sodium and ammonium chloride are utilized, while nitrate sodium is not reduced. Antimicrobial susceptibility test showed that this strain is susceptible to ampicillin, tetracycline, erythromycin and gentamicin, and resistant to kanamycin.Table 1

Bottom Line: To date, there is only one E. mori genome has been sequenced and very little knowledge about the mechanism of E. mori adapted to the petroleum reservoir.Here, we report the second E. mori genome sequence and annotation, together with the description of features for this organism.The 4,621,281 bp assembly genome exhibits a G + C content of 56.24% and contains 4,317 protein-coding and 65 RNA genes, including 5 rRNA genes.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, School of Energy Resources, China University of Geosciences, Beijing, China.

ABSTRACT
Enterobacter mori strain 5-4 is a Gram-negative, motile, rod shaped, and facultatively anaerobic bacterium, which was isolated from a mixture of formation water (also known as oil-reservior water) and crude-oil in Karamay oilfield, China. To date, there is only one E. mori genome has been sequenced and very little knowledge about the mechanism of E. mori adapted to the petroleum reservoir. Here, we report the second E. mori genome sequence and annotation, together with the description of features for this organism. The 4,621,281 bp assembly genome exhibits a G + C content of 56.24% and contains 4,317 protein-coding and 65 RNA genes, including 5 rRNA genes.

No MeSH data available.


Related in: MedlinePlus