Limits...
Antimicrobial potential of consolidation polymers loaded with biological copper nanoparticles.

Essa AM, Khallaf MK - BMC Microbiol. (2016)

Bottom Line: Biodeterioration of historic monuments and stone works by microorganisms takes place as a result of biofilm production and secretion of organic compounds that negatively affect on the stone matrix.Furthermore, the elemental composition of CuNPs was elucidated using electron dispersive x-ray system connected with the scanning electron microscope.Consolidation polymers impregnated with CuNPs could be used to restrain microbial deterioration in addition to the refinement of physico-mechanical behavior of the historic stones.

View Article: PubMed Central - PubMed

Affiliation: Botany Department, Faculty of Science, Fayoum University, Fayoum, Egypt. ashraf.essa@yahoo.com.

ABSTRACT

Background: Biodeterioration of historic monuments and stone works by microorganisms takes place as a result of biofilm production and secretion of organic compounds that negatively affect on the stone matrix.

Methods: Copper nanoparticles (CuNPs) were prepared biologically using the headspace gases generated by the bacterial culture Escherichia coli Z1. The antimicrobial activity of CuNPs was evaluated against the bacterial strains Bacillus subtilis, Micrococcus luteus, Streptomyces parvulus, Escherichia coli, Pseudomonas aeruginosa as well as some fungal strains Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum, Fusarium solani and Alternaria solani.

Results: Biological CuNPs demonstrated antibacterial and antifungal activities higher than those of the untreated copper sulfate. At the same time, limestone and sandstone blocks treated with consolidation polymers functionalized with CuNPs recorded apparent antimicrobial activity against E. coli, S. parvulus and B. subtilis in addition to an improvement in the physical and mechanical characters of the treated stones. Furthermore, the elemental composition of CuNPs was elucidated using electron dispersive x-ray system connected with the scanning electron microscope.

Conclusion: Consolidation polymers impregnated with CuNPs could be used to restrain microbial deterioration in addition to the refinement of physico-mechanical behavior of the historic stones.

No MeSH data available.


Related in: MedlinePlus

Antimicrobial activity of different concentrations of CuNPs against bacteria (a) and fungi (b). The bacterial growth was monitored as culture optical density (OD600) while the fungal growth was assayed as biomass dry weight. Data are the means of five replication ± standard errors. Means were compared by least significant differences (LSD) test at levels P <0.05 and P <0.01
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4940715&req=5

Fig1: Antimicrobial activity of different concentrations of CuNPs against bacteria (a) and fungi (b). The bacterial growth was monitored as culture optical density (OD600) while the fungal growth was assayed as biomass dry weight. Data are the means of five replication ± standard errors. Means were compared by least significant differences (LSD) test at levels P <0.05 and P <0.01

Mentions: As a result of pumping the biogenic volatiles of the bacterial strain Escherichia coli Z1 in the copper sulfate solution for short exposure time (60 min), a light blue colloidal solution of copper was obtained. The antibacterial potentiality of the collected Cu-particles was demonstrated at different concentrations. Results in Fig. 1a showed a suppression of the bacterial growth at various levels depending on Cu-particles concentrations. There was no bacterial growth at the concentration 150 μg/mL or above while at 100 μg/mL the percentage of growth reduction reached 94.7 % for E. coli, 92.4 % for M. luteus, 90.9 % for S. parvulus, and 95.5 % for B. subtilis. At the same time, P. aeruginosa demonstrated a clear tolerance against high concentrations of Cu-particles where the percentage growth inhibition was 69.6 % at 150 μg/mL and 79.5 % at 200 μg/mL. In order to confirm the antibacterial activity of Cu-particles in comparison with untreated copper sulfate, another experiment was conducted where the diameter of the inhibition zones was measured. The obtained results (Table 1 and Fig. 2) showed an increase of the antibacterial activity of Cu-particles compared to CuSO4. The maximum inhibition zones were recorded with Cu-particles (150 μg/mL) against E. coli Z1 (38 mm) and S. parvulus (33 mm) while the lowest value was recorded with P. aeruginosa (16 mm).Fig. 1


Antimicrobial potential of consolidation polymers loaded with biological copper nanoparticles.

Essa AM, Khallaf MK - BMC Microbiol. (2016)

Antimicrobial activity of different concentrations of CuNPs against bacteria (a) and fungi (b). The bacterial growth was monitored as culture optical density (OD600) while the fungal growth was assayed as biomass dry weight. Data are the means of five replication ± standard errors. Means were compared by least significant differences (LSD) test at levels P <0.05 and P <0.01
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940715&req=5

Fig1: Antimicrobial activity of different concentrations of CuNPs against bacteria (a) and fungi (b). The bacterial growth was monitored as culture optical density (OD600) while the fungal growth was assayed as biomass dry weight. Data are the means of five replication ± standard errors. Means were compared by least significant differences (LSD) test at levels P <0.05 and P <0.01
Mentions: As a result of pumping the biogenic volatiles of the bacterial strain Escherichia coli Z1 in the copper sulfate solution for short exposure time (60 min), a light blue colloidal solution of copper was obtained. The antibacterial potentiality of the collected Cu-particles was demonstrated at different concentrations. Results in Fig. 1a showed a suppression of the bacterial growth at various levels depending on Cu-particles concentrations. There was no bacterial growth at the concentration 150 μg/mL or above while at 100 μg/mL the percentage of growth reduction reached 94.7 % for E. coli, 92.4 % for M. luteus, 90.9 % for S. parvulus, and 95.5 % for B. subtilis. At the same time, P. aeruginosa demonstrated a clear tolerance against high concentrations of Cu-particles where the percentage growth inhibition was 69.6 % at 150 μg/mL and 79.5 % at 200 μg/mL. In order to confirm the antibacterial activity of Cu-particles in comparison with untreated copper sulfate, another experiment was conducted where the diameter of the inhibition zones was measured. The obtained results (Table 1 and Fig. 2) showed an increase of the antibacterial activity of Cu-particles compared to CuSO4. The maximum inhibition zones were recorded with Cu-particles (150 μg/mL) against E. coli Z1 (38 mm) and S. parvulus (33 mm) while the lowest value was recorded with P. aeruginosa (16 mm).Fig. 1

Bottom Line: Biodeterioration of historic monuments and stone works by microorganisms takes place as a result of biofilm production and secretion of organic compounds that negatively affect on the stone matrix.Furthermore, the elemental composition of CuNPs was elucidated using electron dispersive x-ray system connected with the scanning electron microscope.Consolidation polymers impregnated with CuNPs could be used to restrain microbial deterioration in addition to the refinement of physico-mechanical behavior of the historic stones.

View Article: PubMed Central - PubMed

Affiliation: Botany Department, Faculty of Science, Fayoum University, Fayoum, Egypt. ashraf.essa@yahoo.com.

ABSTRACT

Background: Biodeterioration of historic monuments and stone works by microorganisms takes place as a result of biofilm production and secretion of organic compounds that negatively affect on the stone matrix.

Methods: Copper nanoparticles (CuNPs) were prepared biologically using the headspace gases generated by the bacterial culture Escherichia coli Z1. The antimicrobial activity of CuNPs was evaluated against the bacterial strains Bacillus subtilis, Micrococcus luteus, Streptomyces parvulus, Escherichia coli, Pseudomonas aeruginosa as well as some fungal strains Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum, Fusarium solani and Alternaria solani.

Results: Biological CuNPs demonstrated antibacterial and antifungal activities higher than those of the untreated copper sulfate. At the same time, limestone and sandstone blocks treated with consolidation polymers functionalized with CuNPs recorded apparent antimicrobial activity against E. coli, S. parvulus and B. subtilis in addition to an improvement in the physical and mechanical characters of the treated stones. Furthermore, the elemental composition of CuNPs was elucidated using electron dispersive x-ray system connected with the scanning electron microscope.

Conclusion: Consolidation polymers impregnated with CuNPs could be used to restrain microbial deterioration in addition to the refinement of physico-mechanical behavior of the historic stones.

No MeSH data available.


Related in: MedlinePlus