Limits...
H. pylori infection is related to mitochondrial microsatellite instability in gastric carcinogenesis.

Ling X, Zhang H, Shen C, Yan W, Wang P, Feng J, Peng Z, Peng G, Chen W, Fang D - Infect. Agents Cancer (2016)

Bottom Line: The levels of IL-8 were significantly higher in specimens with mtMSI than in those without mtMSI (P < 0.01).An association of mtMSI with the intestinal histological type was found (P < 0.05).Increased IL-8 levels induced by H. pylori were related to the invasion, lymphnode spreading and clinical stage of gastric cancer (P < 0.05).Our results support a role for mtMSI in different mechanisms of gastric carcinogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038 China.

ABSTRACT

Bachground: To assess the correlation of H. pylori infection with mitochondrial microsatellite instability (mtMSI) and IL-8 in gastric carcinogenesis.

Methods: H. pylori infection was evaluated through histology and a urease breath test; mtMSI was measured using PCR-single strand conformation polymorphism (PCR-SSCP); IL-8 was analyzed with ELISA methods.

Results: The detection rate of mtMSI was significantly higher in specimens with H. pylori infection than in those without H. pylori infection (P < 0.05). The levels of IL-8 were significantly higher in specimens with mtMSI than in those without mtMSI (P < 0.01).An association of mtMSI with the intestinal histological type was found (P < 0.05). Increased IL-8 levels induced by H. pylori were related to the invasion, lymphnode spreading and clinical stage of gastric cancer (P < 0.05).

Conclusions: H. pylori infection is related to mitochondrial microsatellite instability in the early steps of gastric cancer development. IL-8 may play a role in the development of mtMSI induced by H. pylori. Our results support a role for mtMSI in different mechanisms of gastric carcinogenesis.

No MeSH data available.


Related in: MedlinePlus

The relationship between H. pylori infection and mtMSI. a. Detection of mtMSI via PCR-single strand conformation polymorphism analysis. Lane 6 shows conformational variants associated with mtMSI. b. The detection rate of mtMSI was significantly higher in specimens with H. pylori infection than in those without H. pylori infection (*P < 0.05)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4940710&req=5

Fig2: The relationship between H. pylori infection and mtMSI. a. Detection of mtMSI via PCR-single strand conformation polymorphism analysis. Lane 6 shows conformational variants associated with mtMSI. b. The detection rate of mtMSI was significantly higher in specimens with H. pylori infection than in those without H. pylori infection (*P < 0.05)

Mentions: All tumor and non-neoplastic gastric mucosal samples were screened for mtMSI at seven repeat sites using the PCR-RFLP method. Fig. 2a shows a representative mobility-shift band compared with normal counterparts. The rates of mtMSI in different gastric pathologies are shown in Fig. 2b. H. pylori infection was associated with increased mtMSI in various gastric pathologies, and the rate of mtMSI was significantly higher in specimens with H. pylori infection than in those without H. pylori infection (P < 0.01).Fig. 2


H. pylori infection is related to mitochondrial microsatellite instability in gastric carcinogenesis.

Ling X, Zhang H, Shen C, Yan W, Wang P, Feng J, Peng Z, Peng G, Chen W, Fang D - Infect. Agents Cancer (2016)

The relationship between H. pylori infection and mtMSI. a. Detection of mtMSI via PCR-single strand conformation polymorphism analysis. Lane 6 shows conformational variants associated with mtMSI. b. The detection rate of mtMSI was significantly higher in specimens with H. pylori infection than in those without H. pylori infection (*P < 0.05)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940710&req=5

Fig2: The relationship between H. pylori infection and mtMSI. a. Detection of mtMSI via PCR-single strand conformation polymorphism analysis. Lane 6 shows conformational variants associated with mtMSI. b. The detection rate of mtMSI was significantly higher in specimens with H. pylori infection than in those without H. pylori infection (*P < 0.05)
Mentions: All tumor and non-neoplastic gastric mucosal samples were screened for mtMSI at seven repeat sites using the PCR-RFLP method. Fig. 2a shows a representative mobility-shift band compared with normal counterparts. The rates of mtMSI in different gastric pathologies are shown in Fig. 2b. H. pylori infection was associated with increased mtMSI in various gastric pathologies, and the rate of mtMSI was significantly higher in specimens with H. pylori infection than in those without H. pylori infection (P < 0.01).Fig. 2

Bottom Line: The levels of IL-8 were significantly higher in specimens with mtMSI than in those without mtMSI (P < 0.01).An association of mtMSI with the intestinal histological type was found (P < 0.05).Increased IL-8 levels induced by H. pylori were related to the invasion, lymphnode spreading and clinical stage of gastric cancer (P < 0.05).Our results support a role for mtMSI in different mechanisms of gastric carcinogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038 China.

ABSTRACT

Bachground: To assess the correlation of H. pylori infection with mitochondrial microsatellite instability (mtMSI) and IL-8 in gastric carcinogenesis.

Methods: H. pylori infection was evaluated through histology and a urease breath test; mtMSI was measured using PCR-single strand conformation polymorphism (PCR-SSCP); IL-8 was analyzed with ELISA methods.

Results: The detection rate of mtMSI was significantly higher in specimens with H. pylori infection than in those without H. pylori infection (P < 0.05). The levels of IL-8 were significantly higher in specimens with mtMSI than in those without mtMSI (P < 0.01).An association of mtMSI with the intestinal histological type was found (P < 0.05). Increased IL-8 levels induced by H. pylori were related to the invasion, lymphnode spreading and clinical stage of gastric cancer (P < 0.05).

Conclusions: H. pylori infection is related to mitochondrial microsatellite instability in the early steps of gastric cancer development. IL-8 may play a role in the development of mtMSI induced by H. pylori. Our results support a role for mtMSI in different mechanisms of gastric carcinogenesis.

No MeSH data available.


Related in: MedlinePlus