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Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle.

Hou C, Miao Y, Wang X, Chen C, Lin B, Hu Z - Exp Ther Med (2016)

Bottom Line: It has been reported that MMP-2, MMP-9 and TIMP-1 are associated with the hair cycle; however, their expression levels during the hair cycle have not been fully elucidated.However, MMP-9 and TIMP-1 were locally expressed in certain areas of the hair follicle, such as in the sebaceous gland at the anagen, catagen and telogen phases, and in the inner root sheath at the catagen phase.These results suggested that MMP-2 and MMP-9 may serve an important role in the hair growth cycle.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT

According to the growth state of hair follicles, the hair cycle is divided into the anagen, catagen and telogen phases. A number of biological factors have been shown to synchronize with the hair cycle. As an important component of the hair follicle, the extracellular matrix is regulated by matrix metalloproteinases (MMPs) and their inhibitors (tissue inhibitor of matrix metalloproteinases; TIMPs). It has been reported that MMP-2, MMP-9 and TIMP-1 are associated with the hair cycle; however, their expression levels during the hair cycle have not been fully elucidated. Reverse transcription-polymerase chain reaction and ELISA analysis in the present study demonstrated that, during the hair cycle in mice, mRNA and protein expression levels of MMP-2 and MMP-9 were elevated in the anagen phase, and decreased during the catagen and telogen phases. Furthermore, SDS-PAGE gelatin zymography demonstrated that their activities fluctuated in the hair cycle. Additionally, it was observed that the mRNA and protein expression levels of TIMP-1 and TIMP-2 were negatively correlated with MMP-9 and MMP-2, respectively. Immunohistochemical examination demonstrated that MMP-2 and TIMP-2 were present in all structures of the hair follicle. However, MMP-9 and TIMP-1 were locally expressed in certain areas of the hair follicle, such as in the sebaceous gland at the anagen, catagen and telogen phases, and in the inner root sheath at the catagen phase. These results suggested that MMP-2 and MMP-9 may serve an important role in the hair growth cycle.

No MeSH data available.


Localization of MMP-2, MMP-9, TIMP-1 and TIMP-2 at each stage of the hair cycle in mice. Brown staining identifies the proteins. MMP, matrix metalloproteinase; TIMP, tissue inhibitor of matrix metalloproteinase.
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f5-etm-0-0-3319: Localization of MMP-2, MMP-9, TIMP-1 and TIMP-2 at each stage of the hair cycle in mice. Brown staining identifies the proteins. MMP, matrix metalloproteinase; TIMP, tissue inhibitor of matrix metalloproteinase.

Mentions: To identify the expression sites of MMP-2, MMP-9, TIMP-1 and TIMP-2 at each stage of the hair cycle, immunohistochemical analysis was performed (Fig. 5). Positive staining for MMP-2 and TIMP-2 was almost observed throughout the entire hair follicle at each stage, although the expression was most intense during the anagen (early catagen) phase. Positive staining was observed for MMP-9 and TIMP-1 locally in the hair follicle, such as in the sebaceous gland during the anagen and catagen phases, and in the inner root sheath during the catagen phase.


Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle.

Hou C, Miao Y, Wang X, Chen C, Lin B, Hu Z - Exp Ther Med (2016)

Localization of MMP-2, MMP-9, TIMP-1 and TIMP-2 at each stage of the hair cycle in mice. Brown staining identifies the proteins. MMP, matrix metalloproteinase; TIMP, tissue inhibitor of matrix metalloproteinase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940682&req=5

f5-etm-0-0-3319: Localization of MMP-2, MMP-9, TIMP-1 and TIMP-2 at each stage of the hair cycle in mice. Brown staining identifies the proteins. MMP, matrix metalloproteinase; TIMP, tissue inhibitor of matrix metalloproteinase.
Mentions: To identify the expression sites of MMP-2, MMP-9, TIMP-1 and TIMP-2 at each stage of the hair cycle, immunohistochemical analysis was performed (Fig. 5). Positive staining for MMP-2 and TIMP-2 was almost observed throughout the entire hair follicle at each stage, although the expression was most intense during the anagen (early catagen) phase. Positive staining was observed for MMP-9 and TIMP-1 locally in the hair follicle, such as in the sebaceous gland during the anagen and catagen phases, and in the inner root sheath during the catagen phase.

Bottom Line: It has been reported that MMP-2, MMP-9 and TIMP-1 are associated with the hair cycle; however, their expression levels during the hair cycle have not been fully elucidated.However, MMP-9 and TIMP-1 were locally expressed in certain areas of the hair follicle, such as in the sebaceous gland at the anagen, catagen and telogen phases, and in the inner root sheath at the catagen phase.These results suggested that MMP-2 and MMP-9 may serve an important role in the hair growth cycle.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT

According to the growth state of hair follicles, the hair cycle is divided into the anagen, catagen and telogen phases. A number of biological factors have been shown to synchronize with the hair cycle. As an important component of the hair follicle, the extracellular matrix is regulated by matrix metalloproteinases (MMPs) and their inhibitors (tissue inhibitor of matrix metalloproteinases; TIMPs). It has been reported that MMP-2, MMP-9 and TIMP-1 are associated with the hair cycle; however, their expression levels during the hair cycle have not been fully elucidated. Reverse transcription-polymerase chain reaction and ELISA analysis in the present study demonstrated that, during the hair cycle in mice, mRNA and protein expression levels of MMP-2 and MMP-9 were elevated in the anagen phase, and decreased during the catagen and telogen phases. Furthermore, SDS-PAGE gelatin zymography demonstrated that their activities fluctuated in the hair cycle. Additionally, it was observed that the mRNA and protein expression levels of TIMP-1 and TIMP-2 were negatively correlated with MMP-9 and MMP-2, respectively. Immunohistochemical examination demonstrated that MMP-2 and TIMP-2 were present in all structures of the hair follicle. However, MMP-9 and TIMP-1 were locally expressed in certain areas of the hair follicle, such as in the sebaceous gland at the anagen, catagen and telogen phases, and in the inner root sheath at the catagen phase. These results suggested that MMP-2 and MMP-9 may serve an important role in the hair growth cycle.

No MeSH data available.