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Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle.

Hou C, Miao Y, Wang X, Chen C, Lin B, Hu Z - Exp Ther Med (2016)

Bottom Line: It has been reported that MMP-2, MMP-9 and TIMP-1 are associated with the hair cycle; however, their expression levels during the hair cycle have not been fully elucidated.However, MMP-9 and TIMP-1 were locally expressed in certain areas of the hair follicle, such as in the sebaceous gland at the anagen, catagen and telogen phases, and in the inner root sheath at the catagen phase.These results suggested that MMP-2 and MMP-9 may serve an important role in the hair growth cycle.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT

According to the growth state of hair follicles, the hair cycle is divided into the anagen, catagen and telogen phases. A number of biological factors have been shown to synchronize with the hair cycle. As an important component of the hair follicle, the extracellular matrix is regulated by matrix metalloproteinases (MMPs) and their inhibitors (tissue inhibitor of matrix metalloproteinases; TIMPs). It has been reported that MMP-2, MMP-9 and TIMP-1 are associated with the hair cycle; however, their expression levels during the hair cycle have not been fully elucidated. Reverse transcription-polymerase chain reaction and ELISA analysis in the present study demonstrated that, during the hair cycle in mice, mRNA and protein expression levels of MMP-2 and MMP-9 were elevated in the anagen phase, and decreased during the catagen and telogen phases. Furthermore, SDS-PAGE gelatin zymography demonstrated that their activities fluctuated in the hair cycle. Additionally, it was observed that the mRNA and protein expression levels of TIMP-1 and TIMP-2 were negatively correlated with MMP-9 and MMP-2, respectively. Immunohistochemical examination demonstrated that MMP-2 and TIMP-2 were present in all structures of the hair follicle. However, MMP-9 and TIMP-1 were locally expressed in certain areas of the hair follicle, such as in the sebaceous gland at the anagen, catagen and telogen phases, and in the inner root sheath at the catagen phase. These results suggested that MMP-2 and MMP-9 may serve an important role in the hair growth cycle.

No MeSH data available.


MMP-2 and MMP-9 activity at each stage of the mouse hair cycle. (A) Gelatin zymography of MMP-2 and MMP-9 activity; quantification of (B) MMP-2 and (C) MMP-9 activity; and (D) immunohistochemical analysis of skin samples at anagen (4 weeks), early catagen (6 weeks) and telogen (10 weeks) phases, stained with hematoxylin and eosin (scale bar, 0.1 mm). MMP, matrix metalloproteinase.
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f2-etm-0-0-3319: MMP-2 and MMP-9 activity at each stage of the mouse hair cycle. (A) Gelatin zymography of MMP-2 and MMP-9 activity; quantification of (B) MMP-2 and (C) MMP-9 activity; and (D) immunohistochemical analysis of skin samples at anagen (4 weeks), early catagen (6 weeks) and telogen (10 weeks) phases, stained with hematoxylin and eosin (scale bar, 0.1 mm). MMP, matrix metalloproteinase.

Mentions: To investigate the correlation between MMP-2, MMP-9 and the hair cycle, the activities of MMP-2 and MMP-9 were determined using samples of dorsal skin, including the hair follicle. As presented in Fig. 2A, the enzyme activities were present at each phase of the hair cycle. The activity of MMP-2 fluctuated in the hair cycle; the activity peaked at 6 and 10 weeks during the anagen phase, decreased during the catagen phase, and reached its lowest level of activity at 7 and 12 weeks during the telogen phase of the hair cycle (Fig. 2B). Similarly, the activity of MMP-9 fluctuated, with a peak at 6 and 10 weeks (Fig. 2C). Typical histopathological images of each stage during the hair cycle are presented in Fig. 2D. Cells in the cytoplasm during the anagen phase were light or mild alkalophilic by eosin coloration, and were eosinophilic with aging or degeneration.


Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle.

Hou C, Miao Y, Wang X, Chen C, Lin B, Hu Z - Exp Ther Med (2016)

MMP-2 and MMP-9 activity at each stage of the mouse hair cycle. (A) Gelatin zymography of MMP-2 and MMP-9 activity; quantification of (B) MMP-2 and (C) MMP-9 activity; and (D) immunohistochemical analysis of skin samples at anagen (4 weeks), early catagen (6 weeks) and telogen (10 weeks) phases, stained with hematoxylin and eosin (scale bar, 0.1 mm). MMP, matrix metalloproteinase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940682&req=5

f2-etm-0-0-3319: MMP-2 and MMP-9 activity at each stage of the mouse hair cycle. (A) Gelatin zymography of MMP-2 and MMP-9 activity; quantification of (B) MMP-2 and (C) MMP-9 activity; and (D) immunohistochemical analysis of skin samples at anagen (4 weeks), early catagen (6 weeks) and telogen (10 weeks) phases, stained with hematoxylin and eosin (scale bar, 0.1 mm). MMP, matrix metalloproteinase.
Mentions: To investigate the correlation between MMP-2, MMP-9 and the hair cycle, the activities of MMP-2 and MMP-9 were determined using samples of dorsal skin, including the hair follicle. As presented in Fig. 2A, the enzyme activities were present at each phase of the hair cycle. The activity of MMP-2 fluctuated in the hair cycle; the activity peaked at 6 and 10 weeks during the anagen phase, decreased during the catagen phase, and reached its lowest level of activity at 7 and 12 weeks during the telogen phase of the hair cycle (Fig. 2B). Similarly, the activity of MMP-9 fluctuated, with a peak at 6 and 10 weeks (Fig. 2C). Typical histopathological images of each stage during the hair cycle are presented in Fig. 2D. Cells in the cytoplasm during the anagen phase were light or mild alkalophilic by eosin coloration, and were eosinophilic with aging or degeneration.

Bottom Line: It has been reported that MMP-2, MMP-9 and TIMP-1 are associated with the hair cycle; however, their expression levels during the hair cycle have not been fully elucidated.However, MMP-9 and TIMP-1 were locally expressed in certain areas of the hair follicle, such as in the sebaceous gland at the anagen, catagen and telogen phases, and in the inner root sheath at the catagen phase.These results suggested that MMP-2 and MMP-9 may serve an important role in the hair growth cycle.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT

According to the growth state of hair follicles, the hair cycle is divided into the anagen, catagen and telogen phases. A number of biological factors have been shown to synchronize with the hair cycle. As an important component of the hair follicle, the extracellular matrix is regulated by matrix metalloproteinases (MMPs) and their inhibitors (tissue inhibitor of matrix metalloproteinases; TIMPs). It has been reported that MMP-2, MMP-9 and TIMP-1 are associated with the hair cycle; however, their expression levels during the hair cycle have not been fully elucidated. Reverse transcription-polymerase chain reaction and ELISA analysis in the present study demonstrated that, during the hair cycle in mice, mRNA and protein expression levels of MMP-2 and MMP-9 were elevated in the anagen phase, and decreased during the catagen and telogen phases. Furthermore, SDS-PAGE gelatin zymography demonstrated that their activities fluctuated in the hair cycle. Additionally, it was observed that the mRNA and protein expression levels of TIMP-1 and TIMP-2 were negatively correlated with MMP-9 and MMP-2, respectively. Immunohistochemical examination demonstrated that MMP-2 and TIMP-2 were present in all structures of the hair follicle. However, MMP-9 and TIMP-1 were locally expressed in certain areas of the hair follicle, such as in the sebaceous gland at the anagen, catagen and telogen phases, and in the inner root sheath at the catagen phase. These results suggested that MMP-2 and MMP-9 may serve an important role in the hair growth cycle.

No MeSH data available.