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Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle.

Hou C, Miao Y, Wang X, Chen C, Lin B, Hu Z - Exp Ther Med (2016)

Bottom Line: It has been reported that MMP-2, MMP-9 and TIMP-1 are associated with the hair cycle; however, their expression levels during the hair cycle have not been fully elucidated.However, MMP-9 and TIMP-1 were locally expressed in certain areas of the hair follicle, such as in the sebaceous gland at the anagen, catagen and telogen phases, and in the inner root sheath at the catagen phase.These results suggested that MMP-2 and MMP-9 may serve an important role in the hair growth cycle.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT

According to the growth state of hair follicles, the hair cycle is divided into the anagen, catagen and telogen phases. A number of biological factors have been shown to synchronize with the hair cycle. As an important component of the hair follicle, the extracellular matrix is regulated by matrix metalloproteinases (MMPs) and their inhibitors (tissue inhibitor of matrix metalloproteinases; TIMPs). It has been reported that MMP-2, MMP-9 and TIMP-1 are associated with the hair cycle; however, their expression levels during the hair cycle have not been fully elucidated. Reverse transcription-polymerase chain reaction and ELISA analysis in the present study demonstrated that, during the hair cycle in mice, mRNA and protein expression levels of MMP-2 and MMP-9 were elevated in the anagen phase, and decreased during the catagen and telogen phases. Furthermore, SDS-PAGE gelatin zymography demonstrated that their activities fluctuated in the hair cycle. Additionally, it was observed that the mRNA and protein expression levels of TIMP-1 and TIMP-2 were negatively correlated with MMP-9 and MMP-2, respectively. Immunohistochemical examination demonstrated that MMP-2 and TIMP-2 were present in all structures of the hair follicle. However, MMP-9 and TIMP-1 were locally expressed in certain areas of the hair follicle, such as in the sebaceous gland at the anagen, catagen and telogen phases, and in the inner root sheath at the catagen phase. These results suggested that MMP-2 and MMP-9 may serve an important role in the hair growth cycle.

No MeSH data available.


Time-scale of the hair cycle in female C57BL/6 mice during the first 12 weeks after birth.
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f1-etm-0-0-3319: Time-scale of the hair cycle in female C57BL/6 mice during the first 12 weeks after birth.

Mentions: The hair cycle is an intrinsic and cyclic system of regenerating tissue (1), which is composed of the anagen (phases of rapid growth; 1–3 weeks), catagen (phases of apoptosis-driven regression; ~2 days) and telogen (phases of relative quiescence; ~2 weeks) phases (Fig. 1). The growth of hair is considered to be a result of the cyclic remodeling of the follicle (2). There are numerous biological factors that regulate or synchronize with the hair cycle (3), such as hormones, growth factors, enzymes and transcriptional factors. Enzymes include ornithine decarboxylase, aromatase and nexin 1. The hair cycle is considered to be a process of tissue regeneration associated with extracellular matrix (ECM) degradation and remodeling (4).


Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in the hair cycle.

Hou C, Miao Y, Wang X, Chen C, Lin B, Hu Z - Exp Ther Med (2016)

Time-scale of the hair cycle in female C57BL/6 mice during the first 12 weeks after birth.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940682&req=5

f1-etm-0-0-3319: Time-scale of the hair cycle in female C57BL/6 mice during the first 12 weeks after birth.
Mentions: The hair cycle is an intrinsic and cyclic system of regenerating tissue (1), which is composed of the anagen (phases of rapid growth; 1–3 weeks), catagen (phases of apoptosis-driven regression; ~2 days) and telogen (phases of relative quiescence; ~2 weeks) phases (Fig. 1). The growth of hair is considered to be a result of the cyclic remodeling of the follicle (2). There are numerous biological factors that regulate or synchronize with the hair cycle (3), such as hormones, growth factors, enzymes and transcriptional factors. Enzymes include ornithine decarboxylase, aromatase and nexin 1. The hair cycle is considered to be a process of tissue regeneration associated with extracellular matrix (ECM) degradation and remodeling (4).

Bottom Line: It has been reported that MMP-2, MMP-9 and TIMP-1 are associated with the hair cycle; however, their expression levels during the hair cycle have not been fully elucidated.However, MMP-9 and TIMP-1 were locally expressed in certain areas of the hair follicle, such as in the sebaceous gland at the anagen, catagen and telogen phases, and in the inner root sheath at the catagen phase.These results suggested that MMP-2 and MMP-9 may serve an important role in the hair growth cycle.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT

According to the growth state of hair follicles, the hair cycle is divided into the anagen, catagen and telogen phases. A number of biological factors have been shown to synchronize with the hair cycle. As an important component of the hair follicle, the extracellular matrix is regulated by matrix metalloproteinases (MMPs) and their inhibitors (tissue inhibitor of matrix metalloproteinases; TIMPs). It has been reported that MMP-2, MMP-9 and TIMP-1 are associated with the hair cycle; however, their expression levels during the hair cycle have not been fully elucidated. Reverse transcription-polymerase chain reaction and ELISA analysis in the present study demonstrated that, during the hair cycle in mice, mRNA and protein expression levels of MMP-2 and MMP-9 were elevated in the anagen phase, and decreased during the catagen and telogen phases. Furthermore, SDS-PAGE gelatin zymography demonstrated that their activities fluctuated in the hair cycle. Additionally, it was observed that the mRNA and protein expression levels of TIMP-1 and TIMP-2 were negatively correlated with MMP-9 and MMP-2, respectively. Immunohistochemical examination demonstrated that MMP-2 and TIMP-2 were present in all structures of the hair follicle. However, MMP-9 and TIMP-1 were locally expressed in certain areas of the hair follicle, such as in the sebaceous gland at the anagen, catagen and telogen phases, and in the inner root sheath at the catagen phase. These results suggested that MMP-2 and MMP-9 may serve an important role in the hair growth cycle.

No MeSH data available.