Limits...
Genome Wide Association Mapping in Arabidopsis thaliana Identifies Novel Genes Involved in Linking Allyl Glucosinolate to Altered Biomass and Defense.

Francisco M, Joseph B, Caligagan H, Li B, Corwin JA, Lin C, Kerwin RE, Burow M, Kliebenstein DJ - Front Plant Sci (2016)

Bottom Line: To start developing a deeper understanding of the mechanism(s) that modulate the ability of exogenous allyl GSL to alter growth and defense, we measured changes in plant biomass and defense metabolites in a collection of natural 96 A. thaliana accessions fed with 50 μM of allyl GSL.Exogenous allyl GSL was introduced exclusively to the roots and the compound transported to the leaf leading to a wide range of heritable effects upon plant biomass and endogenous GSL accumulation.This is one of the first instances in which this approach has been successfully utilized to begin dissecting a novel phenotype to the underlying molecular/polygenic basis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California, DavisDavis, CA, USA; Group of Genetics, Breeding and Biochemistry of Brassicas, Department of Plant Genetics, Misión Biológica de Galicia, Spanish Council for Scientific ResearchPontevedra, Spain.

ABSTRACT
A key limitation in modern biology is the ability to rapidly identify genes underlying newly identified complex phenotypes. Genome wide association studies (GWAS) have become an increasingly important approach for dissecting natural variation by associating phenotypes with genotypes at a genome wide level. Recent work is showing that the Arabidopsis thaliana defense metabolite, allyl glucosinolate (GSL), may provide direct feedback regulation, linking defense metabolism outputs to the growth, and defense responses of the plant. However, there is still a need to identify genes that underlie this process. To start developing a deeper understanding of the mechanism(s) that modulate the ability of exogenous allyl GSL to alter growth and defense, we measured changes in plant biomass and defense metabolites in a collection of natural 96 A. thaliana accessions fed with 50 μM of allyl GSL. Exogenous allyl GSL was introduced exclusively to the roots and the compound transported to the leaf leading to a wide range of heritable effects upon plant biomass and endogenous GSL accumulation. Using natural variation we conducted GWAS to identify a number of new genes which potentially control allyl responses in various plant processes. This is one of the first instances in which this approach has been successfully utilized to begin dissecting a novel phenotype to the underlying molecular/polygenic basis.

No MeSH data available.


Related in: MedlinePlus

Natural variation in Arabidopsis biomass and GSL accumulation in response to exogenous allyl GSL. (A) Kernel density plots showing the distribution of fw (mg/plant), (B) total aliphatic GSLs, and (C) total indolic GSLs (μmol/g of fw) from 96 natural Arabidopsis accessions grown in MS (black line) and MS + Allyl (red line).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4940622&req=5

Figure 1: Natural variation in Arabidopsis biomass and GSL accumulation in response to exogenous allyl GSL. (A) Kernel density plots showing the distribution of fw (mg/plant), (B) total aliphatic GSLs, and (C) total indolic GSLs (μmol/g of fw) from 96 natural Arabidopsis accessions grown in MS (black line) and MS + Allyl (red line).

Mentions: To begin identifying genes and the potential mechanism(s) by which allyl GSL can affect biomass changes in Arabidopsis, we measured the response of a population of 96 natural Arabidopsis accessions to external allyl GSL application. All accessions were planted in quintuplicate using a random split-block design. This population was previously analyzed to assess how endogenous GSL genetic variation influences the link between allyl GSL and biomass accumulation but the genetic architecture of these traits have not yet been described (Francisco et al., 2016). ANOVA showed that plant biomass was highly heritable (H2 = 0.88) and that natural Arabidopsis accessions have significant variation for the effect of allyl GSL upon seedling plant biomass (Table 2). The distribution of plant biomass across the accessions showed that, in general, exogenous allyl GSL application decreased plant biomass across the population but individual accessions showed positive responses (Figure 1). Thus, there is genetic variation for the plant biomass response to exogenous allyl GSL application in A. thaliana. Further, the presence of accessions with positive and negative responses to allyl GSL suggests that there is likely more than one mechanism controlling the response.


Genome Wide Association Mapping in Arabidopsis thaliana Identifies Novel Genes Involved in Linking Allyl Glucosinolate to Altered Biomass and Defense.

Francisco M, Joseph B, Caligagan H, Li B, Corwin JA, Lin C, Kerwin RE, Burow M, Kliebenstein DJ - Front Plant Sci (2016)

Natural variation in Arabidopsis biomass and GSL accumulation in response to exogenous allyl GSL. (A) Kernel density plots showing the distribution of fw (mg/plant), (B) total aliphatic GSLs, and (C) total indolic GSLs (μmol/g of fw) from 96 natural Arabidopsis accessions grown in MS (black line) and MS + Allyl (red line).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940622&req=5

Figure 1: Natural variation in Arabidopsis biomass and GSL accumulation in response to exogenous allyl GSL. (A) Kernel density plots showing the distribution of fw (mg/plant), (B) total aliphatic GSLs, and (C) total indolic GSLs (μmol/g of fw) from 96 natural Arabidopsis accessions grown in MS (black line) and MS + Allyl (red line).
Mentions: To begin identifying genes and the potential mechanism(s) by which allyl GSL can affect biomass changes in Arabidopsis, we measured the response of a population of 96 natural Arabidopsis accessions to external allyl GSL application. All accessions were planted in quintuplicate using a random split-block design. This population was previously analyzed to assess how endogenous GSL genetic variation influences the link between allyl GSL and biomass accumulation but the genetic architecture of these traits have not yet been described (Francisco et al., 2016). ANOVA showed that plant biomass was highly heritable (H2 = 0.88) and that natural Arabidopsis accessions have significant variation for the effect of allyl GSL upon seedling plant biomass (Table 2). The distribution of plant biomass across the accessions showed that, in general, exogenous allyl GSL application decreased plant biomass across the population but individual accessions showed positive responses (Figure 1). Thus, there is genetic variation for the plant biomass response to exogenous allyl GSL application in A. thaliana. Further, the presence of accessions with positive and negative responses to allyl GSL suggests that there is likely more than one mechanism controlling the response.

Bottom Line: To start developing a deeper understanding of the mechanism(s) that modulate the ability of exogenous allyl GSL to alter growth and defense, we measured changes in plant biomass and defense metabolites in a collection of natural 96 A. thaliana accessions fed with 50 μM of allyl GSL.Exogenous allyl GSL was introduced exclusively to the roots and the compound transported to the leaf leading to a wide range of heritable effects upon plant biomass and endogenous GSL accumulation.This is one of the first instances in which this approach has been successfully utilized to begin dissecting a novel phenotype to the underlying molecular/polygenic basis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California, DavisDavis, CA, USA; Group of Genetics, Breeding and Biochemistry of Brassicas, Department of Plant Genetics, Misión Biológica de Galicia, Spanish Council for Scientific ResearchPontevedra, Spain.

ABSTRACT
A key limitation in modern biology is the ability to rapidly identify genes underlying newly identified complex phenotypes. Genome wide association studies (GWAS) have become an increasingly important approach for dissecting natural variation by associating phenotypes with genotypes at a genome wide level. Recent work is showing that the Arabidopsis thaliana defense metabolite, allyl glucosinolate (GSL), may provide direct feedback regulation, linking defense metabolism outputs to the growth, and defense responses of the plant. However, there is still a need to identify genes that underlie this process. To start developing a deeper understanding of the mechanism(s) that modulate the ability of exogenous allyl GSL to alter growth and defense, we measured changes in plant biomass and defense metabolites in a collection of natural 96 A. thaliana accessions fed with 50 μM of allyl GSL. Exogenous allyl GSL was introduced exclusively to the roots and the compound transported to the leaf leading to a wide range of heritable effects upon plant biomass and endogenous GSL accumulation. Using natural variation we conducted GWAS to identify a number of new genes which potentially control allyl responses in various plant processes. This is one of the first instances in which this approach has been successfully utilized to begin dissecting a novel phenotype to the underlying molecular/polygenic basis.

No MeSH data available.


Related in: MedlinePlus