Limits...
Genetic Diversity of Namibian Pennisetum glaucum (L.) R. BR. (Pearl Millet) Landraces Analyzed by SSR and Morphological Markers.

McBenedict B, Chimwamurombe P, Kwembeya E, Maggs-Kölling G - ScientificWorldJournal (2016)

Bottom Line: Ordination using Principal Coordinate Analysis (PCoA) on SSR data confirmed clusters generated by UPGMA for the 96 P. glaucum accessions.Lodging susceptibility, tillering attitude, spike density, fodder yield potential, early vigour, and spike shape were the phenotypic characters upon which some clusters were based in both datasets.It is recommended that efforts should be made to widen the current gene pool in Namibia.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, University of Namibia, 340 Mandume Ndemufayo Avenue, Windhoek, Namibia.

ABSTRACT
Current Pennisetum glaucum (L.) R. BR. cultivars in Namibia have overall poor performance posing a threat to the nation's food security because this crop is staple for over 70% of the Namibian population. The crop suffers from undesirable production traits such as susceptibility to diseases, low yield, and prolonged reproductive cycle. This study aimed to understand the genetic diversity of the crop in Namibia by simple sequence repeats (SSRs) and morphology analysis. A total of 1441 genotypes were collected from the National Gene Bank representing all the Namibian landraces. A sample of 96 genotypes was further analyzed by SSR using Shannon-Wiener diversity index and revealed a value of 0.45 indicating low genetic diversity. Ordination using Principal Coordinate Analysis (PCoA) on SSR data confirmed clusters generated by UPGMA for the 96 P. glaucum accessions. UPGMA phenograms of 29 morphological characterized genotypes were generated for SSR and morphology data and the two trees revealed 78% resemblance. Lodging susceptibility, tillering attitude, spike density, fodder yield potential, early vigour, and spike shape were the phenotypic characters upon which some clusters were based in both datasets. It is recommended that efforts should be made to widen the current gene pool in Namibia.

No MeSH data available.


Related in: MedlinePlus

UPGMA phenogram constructed from 3005, 3035, and 3039 SSR profiles showing some phenotypic traits of 29 P. glaucum individuals. The scale represents Bray-Curtis similarity coefficients.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4940581&req=5

fig5: UPGMA phenogram constructed from 3005, 3035, and 3039 SSR profiles showing some phenotypic traits of 29 P. glaucum individuals. The scale represents Bray-Curtis similarity coefficients.

Mentions: (a) SSR UPGMA Clustering of 29 Accessions. An SSR phenogram (Figure 5) was drawn based on the same twenty-nine (29) P. glaucum landraces used for morphology cluster construction. The results showed that the 29 accessions could be grouped into two main groups using SSRs. The two (2) main clusters formed at a similarity level of 69% and were designated (I) for the first main cluster and (II) for the second main cluster. The first main cluster (I) had accessions with an intermediate spike density while subcluster 1 had accessions possessing a candle spike shape. Accessions in subcluster 2 possess a compact spike density whereas subcluster 3 had accessions with an intermediate tillering attitude. Subcluster 4 had accessions with an erect tillering attitude while subcluster 7 had accessions with low lodging susceptibility.


Genetic Diversity of Namibian Pennisetum glaucum (L.) R. BR. (Pearl Millet) Landraces Analyzed by SSR and Morphological Markers.

McBenedict B, Chimwamurombe P, Kwembeya E, Maggs-Kölling G - ScientificWorldJournal (2016)

UPGMA phenogram constructed from 3005, 3035, and 3039 SSR profiles showing some phenotypic traits of 29 P. glaucum individuals. The scale represents Bray-Curtis similarity coefficients.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940581&req=5

fig5: UPGMA phenogram constructed from 3005, 3035, and 3039 SSR profiles showing some phenotypic traits of 29 P. glaucum individuals. The scale represents Bray-Curtis similarity coefficients.
Mentions: (a) SSR UPGMA Clustering of 29 Accessions. An SSR phenogram (Figure 5) was drawn based on the same twenty-nine (29) P. glaucum landraces used for morphology cluster construction. The results showed that the 29 accessions could be grouped into two main groups using SSRs. The two (2) main clusters formed at a similarity level of 69% and were designated (I) for the first main cluster and (II) for the second main cluster. The first main cluster (I) had accessions with an intermediate spike density while subcluster 1 had accessions possessing a candle spike shape. Accessions in subcluster 2 possess a compact spike density whereas subcluster 3 had accessions with an intermediate tillering attitude. Subcluster 4 had accessions with an erect tillering attitude while subcluster 7 had accessions with low lodging susceptibility.

Bottom Line: Ordination using Principal Coordinate Analysis (PCoA) on SSR data confirmed clusters generated by UPGMA for the 96 P. glaucum accessions.Lodging susceptibility, tillering attitude, spike density, fodder yield potential, early vigour, and spike shape were the phenotypic characters upon which some clusters were based in both datasets.It is recommended that efforts should be made to widen the current gene pool in Namibia.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, University of Namibia, 340 Mandume Ndemufayo Avenue, Windhoek, Namibia.

ABSTRACT
Current Pennisetum glaucum (L.) R. BR. cultivars in Namibia have overall poor performance posing a threat to the nation's food security because this crop is staple for over 70% of the Namibian population. The crop suffers from undesirable production traits such as susceptibility to diseases, low yield, and prolonged reproductive cycle. This study aimed to understand the genetic diversity of the crop in Namibia by simple sequence repeats (SSRs) and morphology analysis. A total of 1441 genotypes were collected from the National Gene Bank representing all the Namibian landraces. A sample of 96 genotypes was further analyzed by SSR using Shannon-Wiener diversity index and revealed a value of 0.45 indicating low genetic diversity. Ordination using Principal Coordinate Analysis (PCoA) on SSR data confirmed clusters generated by UPGMA for the 96 P. glaucum accessions. UPGMA phenograms of 29 morphological characterized genotypes were generated for SSR and morphology data and the two trees revealed 78% resemblance. Lodging susceptibility, tillering attitude, spike density, fodder yield potential, early vigour, and spike shape were the phenotypic characters upon which some clusters were based in both datasets. It is recommended that efforts should be made to widen the current gene pool in Namibia.

No MeSH data available.


Related in: MedlinePlus