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Genetic Diversity of Namibian Pennisetum glaucum (L.) R. BR. (Pearl Millet) Landraces Analyzed by SSR and Morphological Markers.

McBenedict B, Chimwamurombe P, Kwembeya E, Maggs-Kölling G - ScientificWorldJournal (2016)

Bottom Line: Ordination using Principal Coordinate Analysis (PCoA) on SSR data confirmed clusters generated by UPGMA for the 96 P. glaucum accessions.Lodging susceptibility, tillering attitude, spike density, fodder yield potential, early vigour, and spike shape were the phenotypic characters upon which some clusters were based in both datasets.It is recommended that efforts should be made to widen the current gene pool in Namibia.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, University of Namibia, 340 Mandume Ndemufayo Avenue, Windhoek, Namibia.

ABSTRACT
Current Pennisetum glaucum (L.) R. BR. cultivars in Namibia have overall poor performance posing a threat to the nation's food security because this crop is staple for over 70% of the Namibian population. The crop suffers from undesirable production traits such as susceptibility to diseases, low yield, and prolonged reproductive cycle. This study aimed to understand the genetic diversity of the crop in Namibia by simple sequence repeats (SSRs) and morphology analysis. A total of 1441 genotypes were collected from the National Gene Bank representing all the Namibian landraces. A sample of 96 genotypes was further analyzed by SSR using Shannon-Wiener diversity index and revealed a value of 0.45 indicating low genetic diversity. Ordination using Principal Coordinate Analysis (PCoA) on SSR data confirmed clusters generated by UPGMA for the 96 P. glaucum accessions. UPGMA phenograms of 29 morphological characterized genotypes were generated for SSR and morphology data and the two trees revealed 78% resemblance. Lodging susceptibility, tillering attitude, spike density, fodder yield potential, early vigour, and spike shape were the phenotypic characters upon which some clusters were based in both datasets. It is recommended that efforts should be made to widen the current gene pool in Namibia.

No MeSH data available.


Related in: MedlinePlus

UPGMA phenogram constructed from morphometric data showing some phenotypic traits of selected 29 P. glaucum individuals. The scale represents Bray-Curtis similarity coefficients.
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Related In: Results  -  Collection


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fig3: UPGMA phenogram constructed from morphometric data showing some phenotypic traits of selected 29 P. glaucum individuals. The scale represents Bray-Curtis similarity coefficients.

Mentions: The genetic diversity of the ninety-six (96) P. glaucum accessions was calculated using the Shannon diversity index based on SSR primers: 3005, 3035, and 3039 (Figure 3). These primers detected a total of 6 alleles designated a1, a2, a3, a4, a5, and a6. The calculated value of the Shannon diversity index was found to be 0.45 (Table 4) indicating a low level of genetic diversity among the accessions. The Shannon diversity index values are usually between 1.5 and 3.5 in most ecological studies, and the index is rarely greater than 4 [13].


Genetic Diversity of Namibian Pennisetum glaucum (L.) R. BR. (Pearl Millet) Landraces Analyzed by SSR and Morphological Markers.

McBenedict B, Chimwamurombe P, Kwembeya E, Maggs-Kölling G - ScientificWorldJournal (2016)

UPGMA phenogram constructed from morphometric data showing some phenotypic traits of selected 29 P. glaucum individuals. The scale represents Bray-Curtis similarity coefficients.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940581&req=5

fig3: UPGMA phenogram constructed from morphometric data showing some phenotypic traits of selected 29 P. glaucum individuals. The scale represents Bray-Curtis similarity coefficients.
Mentions: The genetic diversity of the ninety-six (96) P. glaucum accessions was calculated using the Shannon diversity index based on SSR primers: 3005, 3035, and 3039 (Figure 3). These primers detected a total of 6 alleles designated a1, a2, a3, a4, a5, and a6. The calculated value of the Shannon diversity index was found to be 0.45 (Table 4) indicating a low level of genetic diversity among the accessions. The Shannon diversity index values are usually between 1.5 and 3.5 in most ecological studies, and the index is rarely greater than 4 [13].

Bottom Line: Ordination using Principal Coordinate Analysis (PCoA) on SSR data confirmed clusters generated by UPGMA for the 96 P. glaucum accessions.Lodging susceptibility, tillering attitude, spike density, fodder yield potential, early vigour, and spike shape were the phenotypic characters upon which some clusters were based in both datasets.It is recommended that efforts should be made to widen the current gene pool in Namibia.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, University of Namibia, 340 Mandume Ndemufayo Avenue, Windhoek, Namibia.

ABSTRACT
Current Pennisetum glaucum (L.) R. BR. cultivars in Namibia have overall poor performance posing a threat to the nation's food security because this crop is staple for over 70% of the Namibian population. The crop suffers from undesirable production traits such as susceptibility to diseases, low yield, and prolonged reproductive cycle. This study aimed to understand the genetic diversity of the crop in Namibia by simple sequence repeats (SSRs) and morphology analysis. A total of 1441 genotypes were collected from the National Gene Bank representing all the Namibian landraces. A sample of 96 genotypes was further analyzed by SSR using Shannon-Wiener diversity index and revealed a value of 0.45 indicating low genetic diversity. Ordination using Principal Coordinate Analysis (PCoA) on SSR data confirmed clusters generated by UPGMA for the 96 P. glaucum accessions. UPGMA phenograms of 29 morphological characterized genotypes were generated for SSR and morphology data and the two trees revealed 78% resemblance. Lodging susceptibility, tillering attitude, spike density, fodder yield potential, early vigour, and spike shape were the phenotypic characters upon which some clusters were based in both datasets. It is recommended that efforts should be made to widen the current gene pool in Namibia.

No MeSH data available.


Related in: MedlinePlus