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Resveratrol Reduces the Incidence of Portal Vein System Thrombosis after Splenectomy in a Rat Fibrosis Model.

Xu M, Xue W, Ma Z, Bai J, Wu S - Oxid Med Cell Longev (2016)

Bottom Line: Results.In RES group, significant decreases in platelet aggregation, platelet radical oxygen species (ROS) production, and increase in platelet nitric oxide (NO) synthesis and platelet apoptosis were observed when compared with Splenectomy II (all p < 0.001), while in LMWH group only significant decrease in platelet aggregation was observed.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.

ABSTRACT
Purpose. To investigate the preventive effect of resveratrol (RES) on the formation of portal vein system thrombosis (PVST) in a rat fibrosis model. Methods. A total of 64 male SD rats, weighing 200-300 g, were divided into five groups: Sham operation, Splenectomy I, Splenectomy II, RES, and low molecular weight heparin (LMWH), with the former two groups as nonfibrosis controls. Blood samples were subjected to biochemical assays. Platelet apoptosis was measured by flow cytometry. All rats were euthanized for PVST detection one week after operation. Results. No PVST occurred in nonfibrosis controls. Compared to Splenectomy II, the incidences of PVST in RES and LMWH groups were significantly decreased (both p < 0.05). Two rats in LMWH group died before euthanasia due to intra-abdominal hemorrhage. In RES group, significant decreases in platelet aggregation, platelet radical oxygen species (ROS) production, and increase in platelet nitric oxide (NO) synthesis and platelet apoptosis were observed when compared with Splenectomy II (all p < 0.001), while in LMWH group only significant decrease in platelet aggregation was observed. Conclusion. Prophylactic application of RES could safely reduce the incidence of PVST after splenectomy in cirrhotic rat. Regulation of platelet function and induction of platelet apoptosis might be the underlying mechanisms.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effect of RES on ROS generation in rat platelets. Rats received RES (50 mg/d per nasogastric tube) for 10 days before operation and ROS generation was quantified in collagen (10 μg/mL)-activated platelets. Results are shown as mean ± SD. ∗p < 0.001 compared with Splenectomy group II. MFI: Mean Fluorescence Index.
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fig2: Inhibitory effect of RES on ROS generation in rat platelets. Rats received RES (50 mg/d per nasogastric tube) for 10 days before operation and ROS generation was quantified in collagen (10 μg/mL)-activated platelets. Results are shown as mean ± SD. ∗p < 0.001 compared with Splenectomy group II. MFI: Mean Fluorescence Index.

Mentions: Compared to Sham and Splenectomy group I, ROS formation by collagen-stimulated platelets increased obviously in fibrosis rat 1 week after splenectomy (all p < 0.001). However, this increase in ROS formation was significantly alleviated by in vivo pretreatment with RES (p < 0.001), but not by postoperative administration of Enoxaparin (Figure 2).


Resveratrol Reduces the Incidence of Portal Vein System Thrombosis after Splenectomy in a Rat Fibrosis Model.

Xu M, Xue W, Ma Z, Bai J, Wu S - Oxid Med Cell Longev (2016)

Inhibitory effect of RES on ROS generation in rat platelets. Rats received RES (50 mg/d per nasogastric tube) for 10 days before operation and ROS generation was quantified in collagen (10 μg/mL)-activated platelets. Results are shown as mean ± SD. ∗p < 0.001 compared with Splenectomy group II. MFI: Mean Fluorescence Index.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940576&req=5

fig2: Inhibitory effect of RES on ROS generation in rat platelets. Rats received RES (50 mg/d per nasogastric tube) for 10 days before operation and ROS generation was quantified in collagen (10 μg/mL)-activated platelets. Results are shown as mean ± SD. ∗p < 0.001 compared with Splenectomy group II. MFI: Mean Fluorescence Index.
Mentions: Compared to Sham and Splenectomy group I, ROS formation by collagen-stimulated platelets increased obviously in fibrosis rat 1 week after splenectomy (all p < 0.001). However, this increase in ROS formation was significantly alleviated by in vivo pretreatment with RES (p < 0.001), but not by postoperative administration of Enoxaparin (Figure 2).

Bottom Line: Results.In RES group, significant decreases in platelet aggregation, platelet radical oxygen species (ROS) production, and increase in platelet nitric oxide (NO) synthesis and platelet apoptosis were observed when compared with Splenectomy II (all p < 0.001), while in LMWH group only significant decrease in platelet aggregation was observed.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.

ABSTRACT
Purpose. To investigate the preventive effect of resveratrol (RES) on the formation of portal vein system thrombosis (PVST) in a rat fibrosis model. Methods. A total of 64 male SD rats, weighing 200-300 g, were divided into five groups: Sham operation, Splenectomy I, Splenectomy II, RES, and low molecular weight heparin (LMWH), with the former two groups as nonfibrosis controls. Blood samples were subjected to biochemical assays. Platelet apoptosis was measured by flow cytometry. All rats were euthanized for PVST detection one week after operation. Results. No PVST occurred in nonfibrosis controls. Compared to Splenectomy II, the incidences of PVST in RES and LMWH groups were significantly decreased (both p < 0.05). Two rats in LMWH group died before euthanasia due to intra-abdominal hemorrhage. In RES group, significant decreases in platelet aggregation, platelet radical oxygen species (ROS) production, and increase in platelet nitric oxide (NO) synthesis and platelet apoptosis were observed when compared with Splenectomy II (all p < 0.001), while in LMWH group only significant decrease in platelet aggregation was observed. Conclusion. Prophylactic application of RES could safely reduce the incidence of PVST after splenectomy in cirrhotic rat. Regulation of platelet function and induction of platelet apoptosis might be the underlying mechanisms.

No MeSH data available.


Related in: MedlinePlus