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Resveratrol Reduces the Incidence of Portal Vein System Thrombosis after Splenectomy in a Rat Fibrosis Model.

Xu M, Xue W, Ma Z, Bai J, Wu S - Oxid Med Cell Longev (2016)

Bottom Line: Results.In RES group, significant decreases in platelet aggregation, platelet radical oxygen species (ROS) production, and increase in platelet nitric oxide (NO) synthesis and platelet apoptosis were observed when compared with Splenectomy II (all p < 0.001), while in LMWH group only significant decrease in platelet aggregation was observed.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.

ABSTRACT
Purpose. To investigate the preventive effect of resveratrol (RES) on the formation of portal vein system thrombosis (PVST) in a rat fibrosis model. Methods. A total of 64 male SD rats, weighing 200-300 g, were divided into five groups: Sham operation, Splenectomy I, Splenectomy II, RES, and low molecular weight heparin (LMWH), with the former two groups as nonfibrosis controls. Blood samples were subjected to biochemical assays. Platelet apoptosis was measured by flow cytometry. All rats were euthanized for PVST detection one week after operation. Results. No PVST occurred in nonfibrosis controls. Compared to Splenectomy II, the incidences of PVST in RES and LMWH groups were significantly decreased (both p < 0.05). Two rats in LMWH group died before euthanasia due to intra-abdominal hemorrhage. In RES group, significant decreases in platelet aggregation, platelet radical oxygen species (ROS) production, and increase in platelet nitric oxide (NO) synthesis and platelet apoptosis were observed when compared with Splenectomy II (all p < 0.001), while in LMWH group only significant decrease in platelet aggregation was observed. Conclusion. Prophylactic application of RES could safely reduce the incidence of PVST after splenectomy in cirrhotic rat. Regulation of platelet function and induction of platelet apoptosis might be the underlying mechanisms.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effects of RES and Enoxaparin on rat platelet aggregation. Rats received RES (50 mg/d per nasogastric tube) for 10 days before operation or a short term postoperative administration of Enoxaparin (1.5 mg/kg subcutaneously on POD 1, 2, and 3) and the blood was collected 1 week after operation. Washed platelets aggregation (1 × 108 platelets/mL) stimulated with 10 μg/mL of collagen was determined. Results are shown as mean ± SD. ∗p < 0.001 compared with Splenectomy group II.
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fig1: Inhibitory effects of RES and Enoxaparin on rat platelet aggregation. Rats received RES (50 mg/d per nasogastric tube) for 10 days before operation or a short term postoperative administration of Enoxaparin (1.5 mg/kg subcutaneously on POD 1, 2, and 3) and the blood was collected 1 week after operation. Washed platelets aggregation (1 × 108 platelets/mL) stimulated with 10 μg/mL of collagen was determined. Results are shown as mean ± SD. ∗p < 0.001 compared with Splenectomy group II.

Mentions: Data on the effects of RES and Enoxaparin on platelet aggregation induced by collagen are presented in Figure 1. Platelet aggregation in rats with liver fibrosis 1 week after splenectomy (Splenectomy group II) was significantly higher than that in rats without liver fibrosis (Sham and Splenectomy group I) (all p < 0.001). Both pretreatment with RES (50 mg/d per nasogastric tube for 10 days) and a short term postoperative administration of Enoxaparin (1.5 mg/kg subcutaneously on POD 1, 2, and 3) caused a significant reduction in collagen-induced platelet aggregation 1 week after splenectomy, compared with Splenectomy group II (all p < 0.001).


Resveratrol Reduces the Incidence of Portal Vein System Thrombosis after Splenectomy in a Rat Fibrosis Model.

Xu M, Xue W, Ma Z, Bai J, Wu S - Oxid Med Cell Longev (2016)

Inhibitory effects of RES and Enoxaparin on rat platelet aggregation. Rats received RES (50 mg/d per nasogastric tube) for 10 days before operation or a short term postoperative administration of Enoxaparin (1.5 mg/kg subcutaneously on POD 1, 2, and 3) and the blood was collected 1 week after operation. Washed platelets aggregation (1 × 108 platelets/mL) stimulated with 10 μg/mL of collagen was determined. Results are shown as mean ± SD. ∗p < 0.001 compared with Splenectomy group II.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940576&req=5

fig1: Inhibitory effects of RES and Enoxaparin on rat platelet aggregation. Rats received RES (50 mg/d per nasogastric tube) for 10 days before operation or a short term postoperative administration of Enoxaparin (1.5 mg/kg subcutaneously on POD 1, 2, and 3) and the blood was collected 1 week after operation. Washed platelets aggregation (1 × 108 platelets/mL) stimulated with 10 μg/mL of collagen was determined. Results are shown as mean ± SD. ∗p < 0.001 compared with Splenectomy group II.
Mentions: Data on the effects of RES and Enoxaparin on platelet aggregation induced by collagen are presented in Figure 1. Platelet aggregation in rats with liver fibrosis 1 week after splenectomy (Splenectomy group II) was significantly higher than that in rats without liver fibrosis (Sham and Splenectomy group I) (all p < 0.001). Both pretreatment with RES (50 mg/d per nasogastric tube for 10 days) and a short term postoperative administration of Enoxaparin (1.5 mg/kg subcutaneously on POD 1, 2, and 3) caused a significant reduction in collagen-induced platelet aggregation 1 week after splenectomy, compared with Splenectomy group II (all p < 0.001).

Bottom Line: Results.In RES group, significant decreases in platelet aggregation, platelet radical oxygen species (ROS) production, and increase in platelet nitric oxide (NO) synthesis and platelet apoptosis were observed when compared with Splenectomy II (all p < 0.001), while in LMWH group only significant decrease in platelet aggregation was observed.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.

ABSTRACT
Purpose. To investigate the preventive effect of resveratrol (RES) on the formation of portal vein system thrombosis (PVST) in a rat fibrosis model. Methods. A total of 64 male SD rats, weighing 200-300 g, were divided into five groups: Sham operation, Splenectomy I, Splenectomy II, RES, and low molecular weight heparin (LMWH), with the former two groups as nonfibrosis controls. Blood samples were subjected to biochemical assays. Platelet apoptosis was measured by flow cytometry. All rats were euthanized for PVST detection one week after operation. Results. No PVST occurred in nonfibrosis controls. Compared to Splenectomy II, the incidences of PVST in RES and LMWH groups were significantly decreased (both p < 0.05). Two rats in LMWH group died before euthanasia due to intra-abdominal hemorrhage. In RES group, significant decreases in platelet aggregation, platelet radical oxygen species (ROS) production, and increase in platelet nitric oxide (NO) synthesis and platelet apoptosis were observed when compared with Splenectomy II (all p < 0.001), while in LMWH group only significant decrease in platelet aggregation was observed. Conclusion. Prophylactic application of RES could safely reduce the incidence of PVST after splenectomy in cirrhotic rat. Regulation of platelet function and induction of platelet apoptosis might be the underlying mechanisms.

No MeSH data available.


Related in: MedlinePlus