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Effects of Chinese Propolis in Protecting Bovine Mammary Epithelial Cells against Mastitis Pathogens-Induced Cell Damage.

Wang K, Jin XL, Shen XG, Sun LP, Wu LM, Wei JQ, Marcucci MC, Hu FL, Liu JX - Mediators Inflamm. (2016)

Bottom Line: MAC-T cells treated with bacterial endotoxin (lipopolysaccharide, LPS), heat-inactivated Escherichia coli, and Staphylococcus aureus exhibited significant decreases in cell viability while TNF-α and lipoteichoic acid (LTA) did not.There were also corresponding decreases in expressions of proinflammatory IL-6 and TNF-α mRNA.CP and its polyphenolic active components (primarily caffeic acid phenethyl ester and quercetin) had strong inhibitive effects against NF-κB activation and increased the transcriptional activity of Nrf2-ARE.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
Chinese propolis (CP), an important hive product, can alleviate inflammatory responses. However, little is known regarding the potential of propolis treatment for mastitis control. To investigate the anti-inflammatory effects of CP on bovine mammary epithelial cells (MAC-T), we used a range of pathogens to induce cellular inflammatory damage. Cell viability was determined and expressions of inflammatory/antioxidant genes were measured. Using a cell-based reporter assay system, we evaluated CP and its primary constituents on the NF-κB and Nrf2-ARE transcription activation. MAC-T cells treated with bacterial endotoxin (lipopolysaccharide, LPS), heat-inactivated Escherichia coli, and Staphylococcus aureus exhibited significant decreases in cell viability while TNF-α and lipoteichoic acid (LTA) did not. Pretreatment with CP prevented losses in cell viability associated with the addition of killed bacteria or bacterial endotoxins. There were also corresponding decreases in expressions of proinflammatory IL-6 and TNF-α mRNA. Compared with the mastitis challenged cells, enhanced expressions of antioxidant genes HO-1, Txnrd-1, and GCLM were observed in CP-treated cells. CP and its polyphenolic active components (primarily caffeic acid phenethyl ester and quercetin) had strong inhibitive effects against NF-κB activation and increased the transcriptional activity of Nrf2-ARE. These findings suggest that propolis may be valuable in the control of bovine mastitis.

No MeSH data available.


Related in: MedlinePlus

Effects of CP treatment on cellular antioxidant defense gene expressions following mastitis pathogen challenges in MAC-T cells. Quantitative PCR analysis of cellular antioxidant defense genes, HO-1 (a), Txnrd-1 (b), and GCLM (c), showing gene expressions after 6 h incubation of MAC-T cells with each different mastitis pathogen, including proinflammatory cytokine (TNF-α 25 ng/mL), bacterial cell wall components (LPS, 1 μg/mL; LTA, 10 μg/mL), and heat-killed mastitis strains (E. coli and S. aureus, 107 particles/mL). Ct values of target genes were normalized to the value of β-actin and relative gene expressions in TNF-α control group were arbitrarily set to one. The data are shown as mean ± SD from three independent experiments and were analyzed by one-way ANOVA with the Student-Newman-Keuls method. The means with different superscripts are significantly different (P < 0.05).
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fig3: Effects of CP treatment on cellular antioxidant defense gene expressions following mastitis pathogen challenges in MAC-T cells. Quantitative PCR analysis of cellular antioxidant defense genes, HO-1 (a), Txnrd-1 (b), and GCLM (c), showing gene expressions after 6 h incubation of MAC-T cells with each different mastitis pathogen, including proinflammatory cytokine (TNF-α 25 ng/mL), bacterial cell wall components (LPS, 1 μg/mL; LTA, 10 μg/mL), and heat-killed mastitis strains (E. coli and S. aureus, 107 particles/mL). Ct values of target genes were normalized to the value of β-actin and relative gene expressions in TNF-α control group were arbitrarily set to one. The data are shown as mean ± SD from three independent experiments and were analyzed by one-way ANOVA with the Student-Newman-Keuls method. The means with different superscripts are significantly different (P < 0.05).

Mentions: As shown in Figure 3(c), only GCLM expression was affected by several mastitis pathogens (TNF-α and two heat-killed mastitis strain particles). We noticed that CP treatment in MAC-T cells leads to substantial increases in HO-1, Txnrd-1, and GCLM expressions (P < 0.05). HO-1 expressions were further elevated in CP-pretreated MAC-T cells undergoing mastitis challenges (Figure 3(a)). Upregulated Txnrd-1 was unchanged in CP-treated MAC-T cells (Figure 3(b), P < 0.05), regardless of these different mastitis pathogens. Furthermore, CP treatment itself cannot induce expression of GCLM but it substantially increased in mastitis pathogen challenged cells along with CP. These results indicated that CP could elicit the antioxidant defense system in the bMECs cells undergoing mastitis challenges.


Effects of Chinese Propolis in Protecting Bovine Mammary Epithelial Cells against Mastitis Pathogens-Induced Cell Damage.

Wang K, Jin XL, Shen XG, Sun LP, Wu LM, Wei JQ, Marcucci MC, Hu FL, Liu JX - Mediators Inflamm. (2016)

Effects of CP treatment on cellular antioxidant defense gene expressions following mastitis pathogen challenges in MAC-T cells. Quantitative PCR analysis of cellular antioxidant defense genes, HO-1 (a), Txnrd-1 (b), and GCLM (c), showing gene expressions after 6 h incubation of MAC-T cells with each different mastitis pathogen, including proinflammatory cytokine (TNF-α 25 ng/mL), bacterial cell wall components (LPS, 1 μg/mL; LTA, 10 μg/mL), and heat-killed mastitis strains (E. coli and S. aureus, 107 particles/mL). Ct values of target genes were normalized to the value of β-actin and relative gene expressions in TNF-α control group were arbitrarily set to one. The data are shown as mean ± SD from three independent experiments and were analyzed by one-way ANOVA with the Student-Newman-Keuls method. The means with different superscripts are significantly different (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4940570&req=5

fig3: Effects of CP treatment on cellular antioxidant defense gene expressions following mastitis pathogen challenges in MAC-T cells. Quantitative PCR analysis of cellular antioxidant defense genes, HO-1 (a), Txnrd-1 (b), and GCLM (c), showing gene expressions after 6 h incubation of MAC-T cells with each different mastitis pathogen, including proinflammatory cytokine (TNF-α 25 ng/mL), bacterial cell wall components (LPS, 1 μg/mL; LTA, 10 μg/mL), and heat-killed mastitis strains (E. coli and S. aureus, 107 particles/mL). Ct values of target genes were normalized to the value of β-actin and relative gene expressions in TNF-α control group were arbitrarily set to one. The data are shown as mean ± SD from three independent experiments and were analyzed by one-way ANOVA with the Student-Newman-Keuls method. The means with different superscripts are significantly different (P < 0.05).
Mentions: As shown in Figure 3(c), only GCLM expression was affected by several mastitis pathogens (TNF-α and two heat-killed mastitis strain particles). We noticed that CP treatment in MAC-T cells leads to substantial increases in HO-1, Txnrd-1, and GCLM expressions (P < 0.05). HO-1 expressions were further elevated in CP-pretreated MAC-T cells undergoing mastitis challenges (Figure 3(a)). Upregulated Txnrd-1 was unchanged in CP-treated MAC-T cells (Figure 3(b), P < 0.05), regardless of these different mastitis pathogens. Furthermore, CP treatment itself cannot induce expression of GCLM but it substantially increased in mastitis pathogen challenged cells along with CP. These results indicated that CP could elicit the antioxidant defense system in the bMECs cells undergoing mastitis challenges.

Bottom Line: MAC-T cells treated with bacterial endotoxin (lipopolysaccharide, LPS), heat-inactivated Escherichia coli, and Staphylococcus aureus exhibited significant decreases in cell viability while TNF-α and lipoteichoic acid (LTA) did not.There were also corresponding decreases in expressions of proinflammatory IL-6 and TNF-α mRNA.CP and its polyphenolic active components (primarily caffeic acid phenethyl ester and quercetin) had strong inhibitive effects against NF-κB activation and increased the transcriptional activity of Nrf2-ARE.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
Chinese propolis (CP), an important hive product, can alleviate inflammatory responses. However, little is known regarding the potential of propolis treatment for mastitis control. To investigate the anti-inflammatory effects of CP on bovine mammary epithelial cells (MAC-T), we used a range of pathogens to induce cellular inflammatory damage. Cell viability was determined and expressions of inflammatory/antioxidant genes were measured. Using a cell-based reporter assay system, we evaluated CP and its primary constituents on the NF-κB and Nrf2-ARE transcription activation. MAC-T cells treated with bacterial endotoxin (lipopolysaccharide, LPS), heat-inactivated Escherichia coli, and Staphylococcus aureus exhibited significant decreases in cell viability while TNF-α and lipoteichoic acid (LTA) did not. Pretreatment with CP prevented losses in cell viability associated with the addition of killed bacteria or bacterial endotoxins. There were also corresponding decreases in expressions of proinflammatory IL-6 and TNF-α mRNA. Compared with the mastitis challenged cells, enhanced expressions of antioxidant genes HO-1, Txnrd-1, and GCLM were observed in CP-treated cells. CP and its polyphenolic active components (primarily caffeic acid phenethyl ester and quercetin) had strong inhibitive effects against NF-κB activation and increased the transcriptional activity of Nrf2-ARE. These findings suggest that propolis may be valuable in the control of bovine mastitis.

No MeSH data available.


Related in: MedlinePlus