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Phase 1 Trial of Autologous Bone Marrow Stem Cell Transplantation in Patients with Spinal Cord Injury.

Kakabadze Z, Kipshidze N, Mardaleishvili K, Chutkerashvili G, Chelishvili I, Harders A, Loladze G, Shatirishvili G, Kipshidze N, Chakhunashvili D, Chutkerashvili K - Stem Cells Int (2016)

Bottom Line: Conclusions.Analysis of subsequent treatment results indicated that the transplantation of mononuclear-enriched autologous BMSCs is a feasible and safe technique.However, successful application of the BMSCs in the clinical practice is associated with the necessity of executing more detailed examinations to evaluate the effect of BMSCs on the patients with spinal cord injury.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Anatomy, Tbilisi State Medical University, 0177 Tbilisi, Georgia; N. Kipshidze Central University Hospital, 0177 Tbilisi, Georgia.

ABSTRACT
Introduction. A total of 18 patients, with complete motor deficits and paraplegia caused by thoracic and lumbar spine trauma without muscle atrophy or psychiatric problems, were included into this study. Materials and Methods. The bone marrow was aspirated from the anterior iliac crest under local anesthesia and the mononuclear fraction was isolated by density gradient method. At least 750 million mononuclear-enriched cells, suspended in 2 mL of saline, were infused intrathecally. Results and Discussion. The study reports demonstrated improvement of motor and sensory functions of various degrees observed in 9 of the 18 (50%) cases after bone marrow stem cell transplantation. Measured by the American Spinal Injury Association (ASIA) scale, 7 (78%) out of the 9 patients observed an improvement by one grade, while two cases (22%) saw an improvement by two grades. However, there were no cases in which the condition was improved by three grades. Conclusions. Analysis of subsequent treatment results indicated that the transplantation of mononuclear-enriched autologous BMSCs is a feasible and safe technique. However, successful application of the BMSCs in the clinical practice is associated with the necessity of executing more detailed examinations to evaluate the effect of BMSCs on the patients with spinal cord injury.

No MeSH data available.


Related in: MedlinePlus

Representative picture of flow cytometric analysis. Mononuclear CD45−/CD34−/CD271+/STRO-1+ cells defined as bone marrow mesenchymal stem cell and their enumerated percentage and absolute count. Bone marrow hematopoietic stem cells determined in CD45+/CD34+ mononuclear cell population and their enumerated percentage and absolute count.
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fig1: Representative picture of flow cytometric analysis. Mononuclear CD45−/CD34−/CD271+/STRO-1+ cells defined as bone marrow mesenchymal stem cell and their enumerated percentage and absolute count. Bone marrow hematopoietic stem cells determined in CD45+/CD34+ mononuclear cell population and their enumerated percentage and absolute count.

Mentions: Mononuclear CD45−/CD34−/CD271+/STRO-1+ cells were defined as BM MSCs and their percentage and absolute count were enumerated. Bone marrow hematopoietic stem cells were determined in CD45+/CD34+ mononuclear cell population and their percentage and absolute counts were enumerated (Figure 1). The total amount of autologous bone marrow and the detailed number of final bone marrow stem cell products are shown in Table 1.


Phase 1 Trial of Autologous Bone Marrow Stem Cell Transplantation in Patients with Spinal Cord Injury.

Kakabadze Z, Kipshidze N, Mardaleishvili K, Chutkerashvili G, Chelishvili I, Harders A, Loladze G, Shatirishvili G, Kipshidze N, Chakhunashvili D, Chutkerashvili K - Stem Cells Int (2016)

Representative picture of flow cytometric analysis. Mononuclear CD45−/CD34−/CD271+/STRO-1+ cells defined as bone marrow mesenchymal stem cell and their enumerated percentage and absolute count. Bone marrow hematopoietic stem cells determined in CD45+/CD34+ mononuclear cell population and their enumerated percentage and absolute count.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940566&req=5

fig1: Representative picture of flow cytometric analysis. Mononuclear CD45−/CD34−/CD271+/STRO-1+ cells defined as bone marrow mesenchymal stem cell and their enumerated percentage and absolute count. Bone marrow hematopoietic stem cells determined in CD45+/CD34+ mononuclear cell population and their enumerated percentage and absolute count.
Mentions: Mononuclear CD45−/CD34−/CD271+/STRO-1+ cells were defined as BM MSCs and their percentage and absolute count were enumerated. Bone marrow hematopoietic stem cells were determined in CD45+/CD34+ mononuclear cell population and their percentage and absolute counts were enumerated (Figure 1). The total amount of autologous bone marrow and the detailed number of final bone marrow stem cell products are shown in Table 1.

Bottom Line: Conclusions.Analysis of subsequent treatment results indicated that the transplantation of mononuclear-enriched autologous BMSCs is a feasible and safe technique.However, successful application of the BMSCs in the clinical practice is associated with the necessity of executing more detailed examinations to evaluate the effect of BMSCs on the patients with spinal cord injury.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Anatomy, Tbilisi State Medical University, 0177 Tbilisi, Georgia; N. Kipshidze Central University Hospital, 0177 Tbilisi, Georgia.

ABSTRACT
Introduction. A total of 18 patients, with complete motor deficits and paraplegia caused by thoracic and lumbar spine trauma without muscle atrophy or psychiatric problems, were included into this study. Materials and Methods. The bone marrow was aspirated from the anterior iliac crest under local anesthesia and the mononuclear fraction was isolated by density gradient method. At least 750 million mononuclear-enriched cells, suspended in 2 mL of saline, were infused intrathecally. Results and Discussion. The study reports demonstrated improvement of motor and sensory functions of various degrees observed in 9 of the 18 (50%) cases after bone marrow stem cell transplantation. Measured by the American Spinal Injury Association (ASIA) scale, 7 (78%) out of the 9 patients observed an improvement by one grade, while two cases (22%) saw an improvement by two grades. However, there were no cases in which the condition was improved by three grades. Conclusions. Analysis of subsequent treatment results indicated that the transplantation of mononuclear-enriched autologous BMSCs is a feasible and safe technique. However, successful application of the BMSCs in the clinical practice is associated with the necessity of executing more detailed examinations to evaluate the effect of BMSCs on the patients with spinal cord injury.

No MeSH data available.


Related in: MedlinePlus