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Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis.

Atabo S, Umar IA, James DB, Mamman AI - Scientifica (Cairo) (2016)

Bottom Line: Partially purified fractions recorded improved antisickling effect (peak activity of 70%).In the membrane stabilizing assay, methanolic and aqueous stem extracts of T. occidentalis showed the highest effect of 71.85% and 61.29%, respectively.The results provide scientific evidence for ethnopharmacological use of T. occidentalis in the management of SCD.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.

ABSTRACT
Background and Purpose. Traditional management of sickle cell disease (SCD) is ubiquitous in Africa. In south-eastern Nigeria, Telfairia occidentalis (T. occidentalis) is strongly recommended for consumption by SCD patients, owing to its presumed therapeutic effect. This study investigates the antisickling and membrane regenerative potentials of T. occidentalis in sickled erythrocytes. Experimental Approach. Sickled erythrocytes obtained from SCD patients were treated with sodium metabisulphite (2%) to induce further sickling. Heat and hypotonic-induced lyses of red blood cells' membranes were also carried out. The RBCs were treated with varying concentration (10.0, 1.0, and 0.1 mg mL(-1) and 0.5, 1.0, 1.5, 2.0, and 2.5 mg mL(-1), resp.) of T. occidentalis extracts as treatment regimen for in vitro antisickling and membrane stabilizing assays. Extract with peak activity was purified and reused in antisickling assay. Key Results. The antisickling activity of aqueous and methanolic extracts of leaves, seeds, and stem of Telfairia occidentalis at 10.0, 1.0, and 0.1 mg mL(-1) revealed that the aqueous leaves extract (10 mg mL(-1)) exhibited the highest antisickling activity (64.03%) which was significantly (p < 0.05) higher than that of the stem (47.30%) and seeds (37.50%). Partially purified fractions recorded improved antisickling effect (peak activity of 70%). Characterization (using GC-MS) of the most active fraction revealed some bioactive compounds. In the membrane stabilizing assay, methanolic and aqueous stem extracts of T. occidentalis showed the highest effect of 71.85% and 61.29%, respectively. Conclusions and Implications. The results provide scientific evidence for ethnopharmacological use of T. occidentalis in the management of SCD.

No MeSH data available.


Related in: MedlinePlus

Percentage cells unsickled with time by 10 mg mL−1 extracts of leaves, seeds, and stem. LEA: aqueous leaves extract, LEM: methanolic leaves extract, SEA: aqueous seed extract, SEM: methanolic seeds extract, STA: aqueous stem extract, STM: methanolic stem extract, NS: normal saline, and PABA: Para-hydroxybenzoic acid.
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fig1: Percentage cells unsickled with time by 10 mg mL−1 extracts of leaves, seeds, and stem. LEA: aqueous leaves extract, LEM: methanolic leaves extract, SEA: aqueous seed extract, SEM: methanolic seeds extract, STA: aqueous stem extract, STM: methanolic stem extract, NS: normal saline, and PABA: Para-hydroxybenzoic acid.

Mentions: The procedure described by [20] was used for smear preparation and counting of sickled and unsickled cells. Briefly, each sample was smeared on microscope slide, fixed with 95% methanol, dried, and stained with Giemsa stain. It was then examined under an oil immersion microscope and counting red blood cells was carried out by viewing from different fields (4 fields) across the slide. The numbers of both sickled and unsickled red blood cells were counted and the percentage of cells unsickled was determined (Figure 1).


Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis.

Atabo S, Umar IA, James DB, Mamman AI - Scientifica (Cairo) (2016)

Percentage cells unsickled with time by 10 mg mL−1 extracts of leaves, seeds, and stem. LEA: aqueous leaves extract, LEM: methanolic leaves extract, SEA: aqueous seed extract, SEM: methanolic seeds extract, STA: aqueous stem extract, STM: methanolic stem extract, NS: normal saline, and PABA: Para-hydroxybenzoic acid.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940565&req=5

fig1: Percentage cells unsickled with time by 10 mg mL−1 extracts of leaves, seeds, and stem. LEA: aqueous leaves extract, LEM: methanolic leaves extract, SEA: aqueous seed extract, SEM: methanolic seeds extract, STA: aqueous stem extract, STM: methanolic stem extract, NS: normal saline, and PABA: Para-hydroxybenzoic acid.
Mentions: The procedure described by [20] was used for smear preparation and counting of sickled and unsickled cells. Briefly, each sample was smeared on microscope slide, fixed with 95% methanol, dried, and stained with Giemsa stain. It was then examined under an oil immersion microscope and counting red blood cells was carried out by viewing from different fields (4 fields) across the slide. The numbers of both sickled and unsickled red blood cells were counted and the percentage of cells unsickled was determined (Figure 1).

Bottom Line: Partially purified fractions recorded improved antisickling effect (peak activity of 70%).In the membrane stabilizing assay, methanolic and aqueous stem extracts of T. occidentalis showed the highest effect of 71.85% and 61.29%, respectively.The results provide scientific evidence for ethnopharmacological use of T. occidentalis in the management of SCD.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.

ABSTRACT
Background and Purpose. Traditional management of sickle cell disease (SCD) is ubiquitous in Africa. In south-eastern Nigeria, Telfairia occidentalis (T. occidentalis) is strongly recommended for consumption by SCD patients, owing to its presumed therapeutic effect. This study investigates the antisickling and membrane regenerative potentials of T. occidentalis in sickled erythrocytes. Experimental Approach. Sickled erythrocytes obtained from SCD patients were treated with sodium metabisulphite (2%) to induce further sickling. Heat and hypotonic-induced lyses of red blood cells' membranes were also carried out. The RBCs were treated with varying concentration (10.0, 1.0, and 0.1 mg mL(-1) and 0.5, 1.0, 1.5, 2.0, and 2.5 mg mL(-1), resp.) of T. occidentalis extracts as treatment regimen for in vitro antisickling and membrane stabilizing assays. Extract with peak activity was purified and reused in antisickling assay. Key Results. The antisickling activity of aqueous and methanolic extracts of leaves, seeds, and stem of Telfairia occidentalis at 10.0, 1.0, and 0.1 mg mL(-1) revealed that the aqueous leaves extract (10 mg mL(-1)) exhibited the highest antisickling activity (64.03%) which was significantly (p < 0.05) higher than that of the stem (47.30%) and seeds (37.50%). Partially purified fractions recorded improved antisickling effect (peak activity of 70%). Characterization (using GC-MS) of the most active fraction revealed some bioactive compounds. In the membrane stabilizing assay, methanolic and aqueous stem extracts of T. occidentalis showed the highest effect of 71.85% and 61.29%, respectively. Conclusions and Implications. The results provide scientific evidence for ethnopharmacological use of T. occidentalis in the management of SCD.

No MeSH data available.


Related in: MedlinePlus