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Application of Hydrophilic Interaction Liquid Chromatography for the Quantification of Flavonoids in Genista tinctoria Extract.

Sentkowska A, Biesaga M, Pyrzynska K - J Anal Methods Chem (2016)

Bottom Line: In the case of bare silica column more or less apparent dual retention mechanism was observed, depending on the water component content in the mobile phase.ZIC-HILIC showed better selectivity (in comparison to silica column) with the detection limit of 0.01 mg/L (only for rutin was 0.05 mg/L).Finally, this chromatographic procedure was validated and applied for the determination of some flavonoids in Genista tinctoria L. extract.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.

ABSTRACT
Hydrophilic interaction chromatography (HILIC) was employed to investigate chromatographic behavior of selected flavonoids from their different subgroups differing in polarity. Chromatographic measurements were performed on two different HILIC columns: unmodified silica (Atlantis-HILIC) and zwitterionic sulfoalkylbetaine (SeQuant ZIC-HILIC). Separation parameters such as content and type of organic modifier were studied. On ZIC column retention factors were observed to be inversely proportional to the buffer content in the mobile phase, which is the typical partitioning mechanism. In the case of bare silica column more or less apparent dual retention mechanism was observed, depending on the water component content in the mobile phase. ZIC-HILIC showed better selectivity (in comparison to silica column) with the detection limit of 0.01 mg/L (only for rutin was 0.05 mg/L). Finally, this chromatographic procedure was validated and applied for the determination of some flavonoids in Genista tinctoria L. extract.

No MeSH data available.


Related in: MedlinePlus

The recovery of apigenin and naringenin in Genista tinctoria extract. I: the addition of 0,5 mg of apigenin or 10 mg of naringenin; II: the addition of 1 mg of apigenin or 20 mg of naringenin (mg/g of dried plant in the extract).
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fig6: The recovery of apigenin and naringenin in Genista tinctoria extract. I: the addition of 0,5 mg of apigenin or 10 mg of naringenin; II: the addition of 1 mg of apigenin or 20 mg of naringenin (mg/g of dried plant in the extract).

Mentions: To check the accuracy of the method, recovery experiments were performed. Suitable amounts of each detected compound were added to the sample and then analyzed, using proposed method. The obtained recoveries were in ranged from 81 to 95%. The example chromatograms of apigenin and naringenin are presented in Figure 6.


Application of Hydrophilic Interaction Liquid Chromatography for the Quantification of Flavonoids in Genista tinctoria Extract.

Sentkowska A, Biesaga M, Pyrzynska K - J Anal Methods Chem (2016)

The recovery of apigenin and naringenin in Genista tinctoria extract. I: the addition of 0,5 mg of apigenin or 10 mg of naringenin; II: the addition of 1 mg of apigenin or 20 mg of naringenin (mg/g of dried plant in the extract).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940558&req=5

fig6: The recovery of apigenin and naringenin in Genista tinctoria extract. I: the addition of 0,5 mg of apigenin or 10 mg of naringenin; II: the addition of 1 mg of apigenin or 20 mg of naringenin (mg/g of dried plant in the extract).
Mentions: To check the accuracy of the method, recovery experiments were performed. Suitable amounts of each detected compound were added to the sample and then analyzed, using proposed method. The obtained recoveries were in ranged from 81 to 95%. The example chromatograms of apigenin and naringenin are presented in Figure 6.

Bottom Line: In the case of bare silica column more or less apparent dual retention mechanism was observed, depending on the water component content in the mobile phase.ZIC-HILIC showed better selectivity (in comparison to silica column) with the detection limit of 0.01 mg/L (only for rutin was 0.05 mg/L).Finally, this chromatographic procedure was validated and applied for the determination of some flavonoids in Genista tinctoria L. extract.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.

ABSTRACT
Hydrophilic interaction chromatography (HILIC) was employed to investigate chromatographic behavior of selected flavonoids from their different subgroups differing in polarity. Chromatographic measurements were performed on two different HILIC columns: unmodified silica (Atlantis-HILIC) and zwitterionic sulfoalkylbetaine (SeQuant ZIC-HILIC). Separation parameters such as content and type of organic modifier were studied. On ZIC column retention factors were observed to be inversely proportional to the buffer content in the mobile phase, which is the typical partitioning mechanism. In the case of bare silica column more or less apparent dual retention mechanism was observed, depending on the water component content in the mobile phase. ZIC-HILIC showed better selectivity (in comparison to silica column) with the detection limit of 0.01 mg/L (only for rutin was 0.05 mg/L). Finally, this chromatographic procedure was validated and applied for the determination of some flavonoids in Genista tinctoria L. extract.

No MeSH data available.


Related in: MedlinePlus