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Application of Hydrophilic Interaction Liquid Chromatography for the Quantification of Flavonoids in Genista tinctoria Extract.

Sentkowska A, Biesaga M, Pyrzynska K - J Anal Methods Chem (2016)

Bottom Line: In the case of bare silica column more or less apparent dual retention mechanism was observed, depending on the water component content in the mobile phase.ZIC-HILIC showed better selectivity (in comparison to silica column) with the detection limit of 0.01 mg/L (only for rutin was 0.05 mg/L).Finally, this chromatographic procedure was validated and applied for the determination of some flavonoids in Genista tinctoria L. extract.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.

ABSTRACT
Hydrophilic interaction chromatography (HILIC) was employed to investigate chromatographic behavior of selected flavonoids from their different subgroups differing in polarity. Chromatographic measurements were performed on two different HILIC columns: unmodified silica (Atlantis-HILIC) and zwitterionic sulfoalkylbetaine (SeQuant ZIC-HILIC). Separation parameters such as content and type of organic modifier were studied. On ZIC column retention factors were observed to be inversely proportional to the buffer content in the mobile phase, which is the typical partitioning mechanism. In the case of bare silica column more or less apparent dual retention mechanism was observed, depending on the water component content in the mobile phase. ZIC-HILIC showed better selectivity (in comparison to silica column) with the detection limit of 0.01 mg/L (only for rutin was 0.05 mg/L). Finally, this chromatographic procedure was validated and applied for the determination of some flavonoids in Genista tinctoria L. extract.

No MeSH data available.


Related in: MedlinePlus

ESI-MS spectra of selected compounds in the Genista tinctoria extract. Column: ZIC-HILIC (100 × 2.1 mm, 3.5 µm). Elution gradient: 0–4 min 98% ACN, 6-7 min 90%, 8–8.4 min 80%, 8.4–12 min 50%, and 13–20 min 98%.
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fig5: ESI-MS spectra of selected compounds in the Genista tinctoria extract. Column: ZIC-HILIC (100 × 2.1 mm, 3.5 µm). Elution gradient: 0–4 min 98% ACN, 6-7 min 90%, 8–8.4 min 80%, 8.4–12 min 50%, and 13–20 min 98%.

Mentions: Figure 5 shows the obtained ESI-MS/MS chromatograms in SRM mode for selected compounds in the Genista tinctoria extract. The identification of the analytes was performed by checking their MS/MS spectra [22]. Obtained results showed high amount of luteolin, genistein, and naringenin (Table 3). Genista tinctoria owes its antioxidant properties due to the presence of polyphenolic compounds, mainly flavonoids, such as luteolin, genistein, and apigenin, whose presence was previously reported [33, 34]. The proposed method enabled detection of other flavonoids, such as myricetin, naringenin, apigenin, and quercetrin, which were not reported before in the extract of this plant.


Application of Hydrophilic Interaction Liquid Chromatography for the Quantification of Flavonoids in Genista tinctoria Extract.

Sentkowska A, Biesaga M, Pyrzynska K - J Anal Methods Chem (2016)

ESI-MS spectra of selected compounds in the Genista tinctoria extract. Column: ZIC-HILIC (100 × 2.1 mm, 3.5 µm). Elution gradient: 0–4 min 98% ACN, 6-7 min 90%, 8–8.4 min 80%, 8.4–12 min 50%, and 13–20 min 98%.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940558&req=5

fig5: ESI-MS spectra of selected compounds in the Genista tinctoria extract. Column: ZIC-HILIC (100 × 2.1 mm, 3.5 µm). Elution gradient: 0–4 min 98% ACN, 6-7 min 90%, 8–8.4 min 80%, 8.4–12 min 50%, and 13–20 min 98%.
Mentions: Figure 5 shows the obtained ESI-MS/MS chromatograms in SRM mode for selected compounds in the Genista tinctoria extract. The identification of the analytes was performed by checking their MS/MS spectra [22]. Obtained results showed high amount of luteolin, genistein, and naringenin (Table 3). Genista tinctoria owes its antioxidant properties due to the presence of polyphenolic compounds, mainly flavonoids, such as luteolin, genistein, and apigenin, whose presence was previously reported [33, 34]. The proposed method enabled detection of other flavonoids, such as myricetin, naringenin, apigenin, and quercetrin, which were not reported before in the extract of this plant.

Bottom Line: In the case of bare silica column more or less apparent dual retention mechanism was observed, depending on the water component content in the mobile phase.ZIC-HILIC showed better selectivity (in comparison to silica column) with the detection limit of 0.01 mg/L (only for rutin was 0.05 mg/L).Finally, this chromatographic procedure was validated and applied for the determination of some flavonoids in Genista tinctoria L. extract.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.

ABSTRACT
Hydrophilic interaction chromatography (HILIC) was employed to investigate chromatographic behavior of selected flavonoids from their different subgroups differing in polarity. Chromatographic measurements were performed on two different HILIC columns: unmodified silica (Atlantis-HILIC) and zwitterionic sulfoalkylbetaine (SeQuant ZIC-HILIC). Separation parameters such as content and type of organic modifier were studied. On ZIC column retention factors were observed to be inversely proportional to the buffer content in the mobile phase, which is the typical partitioning mechanism. In the case of bare silica column more or less apparent dual retention mechanism was observed, depending on the water component content in the mobile phase. ZIC-HILIC showed better selectivity (in comparison to silica column) with the detection limit of 0.01 mg/L (only for rutin was 0.05 mg/L). Finally, this chromatographic procedure was validated and applied for the determination of some flavonoids in Genista tinctoria L. extract.

No MeSH data available.


Related in: MedlinePlus