Limits...
Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats.

Dembiński A, Warzecha Z, Ceranowicz P, Dembiński M, Cieszkowski J, Gosiewski T, Bulanda M, Kuśnierz-Cabala B, Gałązka K, Konturek PC - Gastroenterol Res Pract (2016)

Bottom Line: This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis.In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.

ABSTRACT
Background. Inflammatory bowel disease results from the dysregulation of immune response to environmental and microbial agents in genetically susceptible individuals. The aim of the present study was to examine the effect of rifaximin and/or Mutaflor (Escherichia coli Nissle 1917, EcN) administration on the healing of acetic acid-induced colitis. Methods. Colitis was induced in male Wistar rats by rectal enema with 3.5% acetic acid solution. Rifaximin (50 mg/kg/dose) and/or Mutaflor (10(9) CFU/dose) were given intragastrically once a day. The severity of colitis was assessed at the 8th day after induction of inflammation. Results. Treatment with rifaximin significantly accelerated the healing of colonic damage. This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis. Moreover, administration of rifaximin significantly reduced concentration of proinflammatory TNF-α and activity of myeloperoxidase in colonic mucosa. Mutaflor given alone was without significant effect on activity of colitis. In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin. Moreover, Mutaflor led to settle of the colon by EcN and this effect was augmented by pretreatment with rifaximin. Conclusion. Rifaximin and Mutaflor exhibit synergic anti-inflammatory and therapeutic effect in acetic acid-induced colitis in rats.

No MeSH data available.


Related in: MedlinePlus

Determination of Muta 5/6 (361 bp), Muta 7/8 (427 bp), and Muta 9/10 (313 bp) amplicons by PCR method as indices of the presence of E. coli Nissle 1917 (EcN). Representative findings in (line 1) control rats without induction of colitis and treated intragastrically (i.g.) with saline; (line 2) rats without induction of colitis and treated i.g. with Mutaflor; (line 3) rats without induction of colitis and treated i.g. with rifaximin; (line 4) rats without induction of colitis and treated i.g. with the combination of rifaximin plus Mutaflor; (line 5) rats treated i.g. with saline after induction of colitis; (line 6) rats treated i.g. with Mutaflor after induction of colitis; (line 7) rats treated i.g. with rifaximin after induction of colitis; and (line 8) rats treated i.g. with the combination of rifaximin plus Mutaflor after induction of colitis. Line M—DNA mass ruler.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4940557&req=5

fig8: Determination of Muta 5/6 (361 bp), Muta 7/8 (427 bp), and Muta 9/10 (313 bp) amplicons by PCR method as indices of the presence of E. coli Nissle 1917 (EcN). Representative findings in (line 1) control rats without induction of colitis and treated intragastrically (i.g.) with saline; (line 2) rats without induction of colitis and treated i.g. with Mutaflor; (line 3) rats without induction of colitis and treated i.g. with rifaximin; (line 4) rats without induction of colitis and treated i.g. with the combination of rifaximin plus Mutaflor; (line 5) rats treated i.g. with saline after induction of colitis; (line 6) rats treated i.g. with Mutaflor after induction of colitis; (line 7) rats treated i.g. with rifaximin after induction of colitis; and (line 8) rats treated i.g. with the combination of rifaximin plus Mutaflor after induction of colitis. Line M—DNA mass ruler.

Mentions: In all rats without treatment with Mutaflor, the presence of E. coli Nissle 1917 (EcN) was not detected (Figure 8). Administration of Mutaflor resulted in colonization of the large intestine by EcN in all rats without induction of colitis. This effect was increased in animals pretreated with rifaximin before Mutaflor administration. In animals with colitis, the colonization of the large intestine by EcN following administration of Mutaflor was less effective. Administration of Mutaflor given alone in rats with colitis led to the presence of primers specific to the EcN only in two cases on 8 observations. The addition of rifaximin before each dose of Mutaflor improved colonization of the colon by EcN. The presence of the EcN was observed in all animals in this experimental group (Figure 8).


Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats.

Dembiński A, Warzecha Z, Ceranowicz P, Dembiński M, Cieszkowski J, Gosiewski T, Bulanda M, Kuśnierz-Cabala B, Gałązka K, Konturek PC - Gastroenterol Res Pract (2016)

Determination of Muta 5/6 (361 bp), Muta 7/8 (427 bp), and Muta 9/10 (313 bp) amplicons by PCR method as indices of the presence of E. coli Nissle 1917 (EcN). Representative findings in (line 1) control rats without induction of colitis and treated intragastrically (i.g.) with saline; (line 2) rats without induction of colitis and treated i.g. with Mutaflor; (line 3) rats without induction of colitis and treated i.g. with rifaximin; (line 4) rats without induction of colitis and treated i.g. with the combination of rifaximin plus Mutaflor; (line 5) rats treated i.g. with saline after induction of colitis; (line 6) rats treated i.g. with Mutaflor after induction of colitis; (line 7) rats treated i.g. with rifaximin after induction of colitis; and (line 8) rats treated i.g. with the combination of rifaximin plus Mutaflor after induction of colitis. Line M—DNA mass ruler.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940557&req=5

fig8: Determination of Muta 5/6 (361 bp), Muta 7/8 (427 bp), and Muta 9/10 (313 bp) amplicons by PCR method as indices of the presence of E. coli Nissle 1917 (EcN). Representative findings in (line 1) control rats without induction of colitis and treated intragastrically (i.g.) with saline; (line 2) rats without induction of colitis and treated i.g. with Mutaflor; (line 3) rats without induction of colitis and treated i.g. with rifaximin; (line 4) rats without induction of colitis and treated i.g. with the combination of rifaximin plus Mutaflor; (line 5) rats treated i.g. with saline after induction of colitis; (line 6) rats treated i.g. with Mutaflor after induction of colitis; (line 7) rats treated i.g. with rifaximin after induction of colitis; and (line 8) rats treated i.g. with the combination of rifaximin plus Mutaflor after induction of colitis. Line M—DNA mass ruler.
Mentions: In all rats without treatment with Mutaflor, the presence of E. coli Nissle 1917 (EcN) was not detected (Figure 8). Administration of Mutaflor resulted in colonization of the large intestine by EcN in all rats without induction of colitis. This effect was increased in animals pretreated with rifaximin before Mutaflor administration. In animals with colitis, the colonization of the large intestine by EcN following administration of Mutaflor was less effective. Administration of Mutaflor given alone in rats with colitis led to the presence of primers specific to the EcN only in two cases on 8 observations. The addition of rifaximin before each dose of Mutaflor improved colonization of the colon by EcN. The presence of the EcN was observed in all animals in this experimental group (Figure 8).

Bottom Line: This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis.In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.

ABSTRACT
Background. Inflammatory bowel disease results from the dysregulation of immune response to environmental and microbial agents in genetically susceptible individuals. The aim of the present study was to examine the effect of rifaximin and/or Mutaflor (Escherichia coli Nissle 1917, EcN) administration on the healing of acetic acid-induced colitis. Methods. Colitis was induced in male Wistar rats by rectal enema with 3.5% acetic acid solution. Rifaximin (50 mg/kg/dose) and/or Mutaflor (10(9) CFU/dose) were given intragastrically once a day. The severity of colitis was assessed at the 8th day after induction of inflammation. Results. Treatment with rifaximin significantly accelerated the healing of colonic damage. This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis. Moreover, administration of rifaximin significantly reduced concentration of proinflammatory TNF-α and activity of myeloperoxidase in colonic mucosa. Mutaflor given alone was without significant effect on activity of colitis. In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin. Moreover, Mutaflor led to settle of the colon by EcN and this effect was augmented by pretreatment with rifaximin. Conclusion. Rifaximin and Mutaflor exhibit synergic anti-inflammatory and therapeutic effect in acetic acid-induced colitis in rats.

No MeSH data available.


Related in: MedlinePlus