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Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats.

Dembiński A, Warzecha Z, Ceranowicz P, Dembiński M, Cieszkowski J, Gosiewski T, Bulanda M, Kuśnierz-Cabala B, Gałązka K, Konturek PC - Gastroenterol Res Pract (2016)

Bottom Line: This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis.In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.

ABSTRACT
Background. Inflammatory bowel disease results from the dysregulation of immune response to environmental and microbial agents in genetically susceptible individuals. The aim of the present study was to examine the effect of rifaximin and/or Mutaflor (Escherichia coli Nissle 1917, EcN) administration on the healing of acetic acid-induced colitis. Methods. Colitis was induced in male Wistar rats by rectal enema with 3.5% acetic acid solution. Rifaximin (50 mg/kg/dose) and/or Mutaflor (10(9) CFU/dose) were given intragastrically once a day. The severity of colitis was assessed at the 8th day after induction of inflammation. Results. Treatment with rifaximin significantly accelerated the healing of colonic damage. This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis. Moreover, administration of rifaximin significantly reduced concentration of proinflammatory TNF-α and activity of myeloperoxidase in colonic mucosa. Mutaflor given alone was without significant effect on activity of colitis. In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin. Moreover, Mutaflor led to settle of the colon by EcN and this effect was augmented by pretreatment with rifaximin. Conclusion. Rifaximin and Mutaflor exhibit synergic anti-inflammatory and therapeutic effect in acetic acid-induced colitis in rats.

No MeSH data available.


Related in: MedlinePlus

Influence of E. coli Nissle 1917 (Mutaflor) and rifaximin on myeloperoxidase activity in colonic mucosa in rats without or with acetic acid-induced colitis. Mean value ± SEM. N = 8 animals in each experimental group and each time of observation. aP < 0.05 compared to control saline-treated rats without induction of colitis; bP < 0.05 compared to colitis + NaCl; cP < 0.05 compared to colitis + Mutaflor; and dP < 0.05 compared to colitis + rifaximin.
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fig7: Influence of E. coli Nissle 1917 (Mutaflor) and rifaximin on myeloperoxidase activity in colonic mucosa in rats without or with acetic acid-induced colitis. Mean value ± SEM. N = 8 animals in each experimental group and each time of observation. aP < 0.05 compared to control saline-treated rats without induction of colitis; bP < 0.05 compared to colitis + NaCl; cP < 0.05 compared to colitis + Mutaflor; and dP < 0.05 compared to colitis + rifaximin.

Mentions: Administration of Mutaflor or rifaximin given alone as well as treatment with the combination of rifaximin plus Mutaflor was without any significant effect on mucosal myeloperoxidase activity in the colon in the rats without colitis induction (Figure 7). Induction of colitis caused more than a 3-fold increase in myeloperoxidase activity in colonic mucosa. Treatment with Mutaflor tended to reduce the colitis-evoked increase in myeloperoxidase activity in the colonic mucosa, but this effect was statistically insignificant. In contrast, administration of rifaximin resulted in a significant reduction in mucosal activity of myeloperoxidase in rats with colitis. Maximal reduction of the colitis-induced increase in mucosal myeloperoxidase activity as observed after treatment with combination of rifaximin and Mutaflor (Figure 7).


Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats.

Dembiński A, Warzecha Z, Ceranowicz P, Dembiński M, Cieszkowski J, Gosiewski T, Bulanda M, Kuśnierz-Cabala B, Gałązka K, Konturek PC - Gastroenterol Res Pract (2016)

Influence of E. coli Nissle 1917 (Mutaflor) and rifaximin on myeloperoxidase activity in colonic mucosa in rats without or with acetic acid-induced colitis. Mean value ± SEM. N = 8 animals in each experimental group and each time of observation. aP < 0.05 compared to control saline-treated rats without induction of colitis; bP < 0.05 compared to colitis + NaCl; cP < 0.05 compared to colitis + Mutaflor; and dP < 0.05 compared to colitis + rifaximin.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940557&req=5

fig7: Influence of E. coli Nissle 1917 (Mutaflor) and rifaximin on myeloperoxidase activity in colonic mucosa in rats without or with acetic acid-induced colitis. Mean value ± SEM. N = 8 animals in each experimental group and each time of observation. aP < 0.05 compared to control saline-treated rats without induction of colitis; bP < 0.05 compared to colitis + NaCl; cP < 0.05 compared to colitis + Mutaflor; and dP < 0.05 compared to colitis + rifaximin.
Mentions: Administration of Mutaflor or rifaximin given alone as well as treatment with the combination of rifaximin plus Mutaflor was without any significant effect on mucosal myeloperoxidase activity in the colon in the rats without colitis induction (Figure 7). Induction of colitis caused more than a 3-fold increase in myeloperoxidase activity in colonic mucosa. Treatment with Mutaflor tended to reduce the colitis-evoked increase in myeloperoxidase activity in the colonic mucosa, but this effect was statistically insignificant. In contrast, administration of rifaximin resulted in a significant reduction in mucosal activity of myeloperoxidase in rats with colitis. Maximal reduction of the colitis-induced increase in mucosal myeloperoxidase activity as observed after treatment with combination of rifaximin and Mutaflor (Figure 7).

Bottom Line: This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis.In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.

ABSTRACT
Background. Inflammatory bowel disease results from the dysregulation of immune response to environmental and microbial agents in genetically susceptible individuals. The aim of the present study was to examine the effect of rifaximin and/or Mutaflor (Escherichia coli Nissle 1917, EcN) administration on the healing of acetic acid-induced colitis. Methods. Colitis was induced in male Wistar rats by rectal enema with 3.5% acetic acid solution. Rifaximin (50 mg/kg/dose) and/or Mutaflor (10(9) CFU/dose) were given intragastrically once a day. The severity of colitis was assessed at the 8th day after induction of inflammation. Results. Treatment with rifaximin significantly accelerated the healing of colonic damage. This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis. Moreover, administration of rifaximin significantly reduced concentration of proinflammatory TNF-α and activity of myeloperoxidase in colonic mucosa. Mutaflor given alone was without significant effect on activity of colitis. In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin. Moreover, Mutaflor led to settle of the colon by EcN and this effect was augmented by pretreatment with rifaximin. Conclusion. Rifaximin and Mutaflor exhibit synergic anti-inflammatory and therapeutic effect in acetic acid-induced colitis in rats.

No MeSH data available.


Related in: MedlinePlus