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Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats.

Dembiński A, Warzecha Z, Ceranowicz P, Dembiński M, Cieszkowski J, Gosiewski T, Bulanda M, Kuśnierz-Cabala B, Gałązka K, Konturek PC - Gastroenterol Res Pract (2016)

Bottom Line: This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis.In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.

ABSTRACT
Background. Inflammatory bowel disease results from the dysregulation of immune response to environmental and microbial agents in genetically susceptible individuals. The aim of the present study was to examine the effect of rifaximin and/or Mutaflor (Escherichia coli Nissle 1917, EcN) administration on the healing of acetic acid-induced colitis. Methods. Colitis was induced in male Wistar rats by rectal enema with 3.5% acetic acid solution. Rifaximin (50 mg/kg/dose) and/or Mutaflor (10(9) CFU/dose) were given intragastrically once a day. The severity of colitis was assessed at the 8th day after induction of inflammation. Results. Treatment with rifaximin significantly accelerated the healing of colonic damage. This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis. Moreover, administration of rifaximin significantly reduced concentration of proinflammatory TNF-α and activity of myeloperoxidase in colonic mucosa. Mutaflor given alone was without significant effect on activity of colitis. In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin. Moreover, Mutaflor led to settle of the colon by EcN and this effect was augmented by pretreatment with rifaximin. Conclusion. Rifaximin and Mutaflor exhibit synergic anti-inflammatory and therapeutic effect in acetic acid-induced colitis in rats.

No MeSH data available.


Related in: MedlinePlus

Influence of E. coli Nissle 1917 (Mutaflor) and rifaximin on of Tumor Necrosis Factor-α concentration in colonic mucosa in rats without or with acetic acid-induced colitis. Mean value ± SEM. N = 8 animals in each experimental group. aP < 0.05 compared to control saline-treated rats without induction of colitis; bP < 0.05 compared to colitis + NaCl; cP < 0.05 compared to colitis + Mutaflor; and dP < 0.05 compared to colitis + rifaximin.
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fig6: Influence of E. coli Nissle 1917 (Mutaflor) and rifaximin on of Tumor Necrosis Factor-α concentration in colonic mucosa in rats without or with acetic acid-induced colitis. Mean value ± SEM. N = 8 animals in each experimental group. aP < 0.05 compared to control saline-treated rats without induction of colitis; bP < 0.05 compared to colitis + NaCl; cP < 0.05 compared to colitis + Mutaflor; and dP < 0.05 compared to colitis + rifaximin.

Mentions: In rats without induction of colitis, intragastric administration of Mutaflor or rifaximin for 7 days failed to affect mucosal concentration of interleukin-1β (IL-1β) or Tumor Necrosis Factor-α (TNF-α) in the colon (Figures 5 and 6, resp.). Induction of colitis caused more than 8-fold increase in concentration of IL-1β and more than 6-fold increase in concentration of TNF-α in colonic mucosa. Administration of Mutaflor after induction of colitis was without significant effect on mucosal concentration of IL-1β or TNF-α in the colon. Rifaximin administered alone caused a partial decrease in the level of IL-1β and TNF-α in colonic mucosa of animals with colitis. However, only the reduction of TNF-α concentration was statistically significant when compared to level observed in rats with colitis treated with saline. Maximal reduction in concentration of IL-1β and TNF-α in colonic mucosa was observed after administration of combination of rifaximin plus Mutaflor (Figures 5 and 6).


Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats.

Dembiński A, Warzecha Z, Ceranowicz P, Dembiński M, Cieszkowski J, Gosiewski T, Bulanda M, Kuśnierz-Cabala B, Gałązka K, Konturek PC - Gastroenterol Res Pract (2016)

Influence of E. coli Nissle 1917 (Mutaflor) and rifaximin on of Tumor Necrosis Factor-α concentration in colonic mucosa in rats without or with acetic acid-induced colitis. Mean value ± SEM. N = 8 animals in each experimental group. aP < 0.05 compared to control saline-treated rats without induction of colitis; bP < 0.05 compared to colitis + NaCl; cP < 0.05 compared to colitis + Mutaflor; and dP < 0.05 compared to colitis + rifaximin.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940557&req=5

fig6: Influence of E. coli Nissle 1917 (Mutaflor) and rifaximin on of Tumor Necrosis Factor-α concentration in colonic mucosa in rats without or with acetic acid-induced colitis. Mean value ± SEM. N = 8 animals in each experimental group. aP < 0.05 compared to control saline-treated rats without induction of colitis; bP < 0.05 compared to colitis + NaCl; cP < 0.05 compared to colitis + Mutaflor; and dP < 0.05 compared to colitis + rifaximin.
Mentions: In rats without induction of colitis, intragastric administration of Mutaflor or rifaximin for 7 days failed to affect mucosal concentration of interleukin-1β (IL-1β) or Tumor Necrosis Factor-α (TNF-α) in the colon (Figures 5 and 6, resp.). Induction of colitis caused more than 8-fold increase in concentration of IL-1β and more than 6-fold increase in concentration of TNF-α in colonic mucosa. Administration of Mutaflor after induction of colitis was without significant effect on mucosal concentration of IL-1β or TNF-α in the colon. Rifaximin administered alone caused a partial decrease in the level of IL-1β and TNF-α in colonic mucosa of animals with colitis. However, only the reduction of TNF-α concentration was statistically significant when compared to level observed in rats with colitis treated with saline. Maximal reduction in concentration of IL-1β and TNF-α in colonic mucosa was observed after administration of combination of rifaximin plus Mutaflor (Figures 5 and 6).

Bottom Line: This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis.In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.

ABSTRACT
Background. Inflammatory bowel disease results from the dysregulation of immune response to environmental and microbial agents in genetically susceptible individuals. The aim of the present study was to examine the effect of rifaximin and/or Mutaflor (Escherichia coli Nissle 1917, EcN) administration on the healing of acetic acid-induced colitis. Methods. Colitis was induced in male Wistar rats by rectal enema with 3.5% acetic acid solution. Rifaximin (50 mg/kg/dose) and/or Mutaflor (10(9) CFU/dose) were given intragastrically once a day. The severity of colitis was assessed at the 8th day after induction of inflammation. Results. Treatment with rifaximin significantly accelerated the healing of colonic damage. This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis. Moreover, administration of rifaximin significantly reduced concentration of proinflammatory TNF-α and activity of myeloperoxidase in colonic mucosa. Mutaflor given alone was without significant effect on activity of colitis. In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin. Moreover, Mutaflor led to settle of the colon by EcN and this effect was augmented by pretreatment with rifaximin. Conclusion. Rifaximin and Mutaflor exhibit synergic anti-inflammatory and therapeutic effect in acetic acid-induced colitis in rats.

No MeSH data available.


Related in: MedlinePlus