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Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats.

Dembiński A, Warzecha Z, Ceranowicz P, Dembiński M, Cieszkowski J, Gosiewski T, Bulanda M, Kuśnierz-Cabala B, Gałązka K, Konturek PC - Gastroenterol Res Pract (2016)

Bottom Line: This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis.In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.

ABSTRACT
Background. Inflammatory bowel disease results from the dysregulation of immune response to environmental and microbial agents in genetically susceptible individuals. The aim of the present study was to examine the effect of rifaximin and/or Mutaflor (Escherichia coli Nissle 1917, EcN) administration on the healing of acetic acid-induced colitis. Methods. Colitis was induced in male Wistar rats by rectal enema with 3.5% acetic acid solution. Rifaximin (50 mg/kg/dose) and/or Mutaflor (10(9) CFU/dose) were given intragastrically once a day. The severity of colitis was assessed at the 8th day after induction of inflammation. Results. Treatment with rifaximin significantly accelerated the healing of colonic damage. This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis. Moreover, administration of rifaximin significantly reduced concentration of proinflammatory TNF-α and activity of myeloperoxidase in colonic mucosa. Mutaflor given alone was without significant effect on activity of colitis. In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin. Moreover, Mutaflor led to settle of the colon by EcN and this effect was augmented by pretreatment with rifaximin. Conclusion. Rifaximin and Mutaflor exhibit synergic anti-inflammatory and therapeutic effect in acetic acid-induced colitis in rats.

No MeSH data available.


Related in: MedlinePlus

Influence of E. coli Nissle 1917 (Mutaflor) and rifaximin on DNA synthesis in colonic mucosa in rats without or with acetic acid-induced colitis. Mean value ± SEM. N = 8 animals in each experimental group. aP < 0.05 compared to control saline-treated rats without induction of colitis; bP < 0.05 compared to colitis + NaCl.
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fig4: Influence of E. coli Nissle 1917 (Mutaflor) and rifaximin on DNA synthesis in colonic mucosa in rats without or with acetic acid-induced colitis. Mean value ± SEM. N = 8 animals in each experimental group. aP < 0.05 compared to control saline-treated rats without induction of colitis; bP < 0.05 compared to colitis + NaCl.

Mentions: In rats without induction of colitis, administration of Mutaflor or rifaximin given alone or in their combination was without any effect on DNA synthesis in colonic mucosa (Figure 4). Induction of colitis by the enema with acetic acid led to reduction in DNA synthesis in colonic mucosa by 38%. Administration of Mutaflor or rifaximin given alone did not significantly affect DNA synthesis in colonic mucosa in rats with colitis. Treatment with the combination of rifaximin plus Mutaflor partly, but significantly reversed the colitis-evoked reduction in mucosal DNA synthesis in the colon (Figure 4).


Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats.

Dembiński A, Warzecha Z, Ceranowicz P, Dembiński M, Cieszkowski J, Gosiewski T, Bulanda M, Kuśnierz-Cabala B, Gałązka K, Konturek PC - Gastroenterol Res Pract (2016)

Influence of E. coli Nissle 1917 (Mutaflor) and rifaximin on DNA synthesis in colonic mucosa in rats without or with acetic acid-induced colitis. Mean value ± SEM. N = 8 animals in each experimental group. aP < 0.05 compared to control saline-treated rats without induction of colitis; bP < 0.05 compared to colitis + NaCl.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940557&req=5

fig4: Influence of E. coli Nissle 1917 (Mutaflor) and rifaximin on DNA synthesis in colonic mucosa in rats without or with acetic acid-induced colitis. Mean value ± SEM. N = 8 animals in each experimental group. aP < 0.05 compared to control saline-treated rats without induction of colitis; bP < 0.05 compared to colitis + NaCl.
Mentions: In rats without induction of colitis, administration of Mutaflor or rifaximin given alone or in their combination was without any effect on DNA synthesis in colonic mucosa (Figure 4). Induction of colitis by the enema with acetic acid led to reduction in DNA synthesis in colonic mucosa by 38%. Administration of Mutaflor or rifaximin given alone did not significantly affect DNA synthesis in colonic mucosa in rats with colitis. Treatment with the combination of rifaximin plus Mutaflor partly, but significantly reversed the colitis-evoked reduction in mucosal DNA synthesis in the colon (Figure 4).

Bottom Line: This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis.In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.

ABSTRACT
Background. Inflammatory bowel disease results from the dysregulation of immune response to environmental and microbial agents in genetically susceptible individuals. The aim of the present study was to examine the effect of rifaximin and/or Mutaflor (Escherichia coli Nissle 1917, EcN) administration on the healing of acetic acid-induced colitis. Methods. Colitis was induced in male Wistar rats by rectal enema with 3.5% acetic acid solution. Rifaximin (50 mg/kg/dose) and/or Mutaflor (10(9) CFU/dose) were given intragastrically once a day. The severity of colitis was assessed at the 8th day after induction of inflammation. Results. Treatment with rifaximin significantly accelerated the healing of colonic damage. This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis. Moreover, administration of rifaximin significantly reduced concentration of proinflammatory TNF-α and activity of myeloperoxidase in colonic mucosa. Mutaflor given alone was without significant effect on activity of colitis. In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin. Moreover, Mutaflor led to settle of the colon by EcN and this effect was augmented by pretreatment with rifaximin. Conclusion. Rifaximin and Mutaflor exhibit synergic anti-inflammatory and therapeutic effect in acetic acid-induced colitis in rats.

No MeSH data available.


Related in: MedlinePlus