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Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats.

Dembiński A, Warzecha Z, Ceranowicz P, Dembiński M, Cieszkowski J, Gosiewski T, Bulanda M, Kuśnierz-Cabala B, Gałązka K, Konturek PC - Gastroenterol Res Pract (2016)

Bottom Line: This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis.In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.

ABSTRACT
Background. Inflammatory bowel disease results from the dysregulation of immune response to environmental and microbial agents in genetically susceptible individuals. The aim of the present study was to examine the effect of rifaximin and/or Mutaflor (Escherichia coli Nissle 1917, EcN) administration on the healing of acetic acid-induced colitis. Methods. Colitis was induced in male Wistar rats by rectal enema with 3.5% acetic acid solution. Rifaximin (50 mg/kg/dose) and/or Mutaflor (10(9) CFU/dose) were given intragastrically once a day. The severity of colitis was assessed at the 8th day after induction of inflammation. Results. Treatment with rifaximin significantly accelerated the healing of colonic damage. This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis. Moreover, administration of rifaximin significantly reduced concentration of proinflammatory TNF-α and activity of myeloperoxidase in colonic mucosa. Mutaflor given alone was without significant effect on activity of colitis. In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin. Moreover, Mutaflor led to settle of the colon by EcN and this effect was augmented by pretreatment with rifaximin. Conclusion. Rifaximin and Mutaflor exhibit synergic anti-inflammatory and therapeutic effect in acetic acid-induced colitis in rats.

No MeSH data available.


Related in: MedlinePlus

Histological features of the rat colonic mucosa stained by haematoxylin and eosin (original magnification 400x). (a) Control rats without induction of colitis and treated with saline for 7 days; (b) rats with colitis treated with saline for 7 days; (c) rats with colitis treated with Mutaflor for 7 days; (d) rats with colitis treated with rifaximin for 7 days; and (e) rats with colitis treated with the combination of rifaximin plus Mutaflor for 7 days.
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fig2: Histological features of the rat colonic mucosa stained by haematoxylin and eosin (original magnification 400x). (a) Control rats without induction of colitis and treated with saline for 7 days; (b) rats with colitis treated with saline for 7 days; (c) rats with colitis treated with Mutaflor for 7 days; (d) rats with colitis treated with rifaximin for 7 days; and (e) rats with colitis treated with the combination of rifaximin plus Mutaflor for 7 days.

Mentions: Macroscopic and microscopic evaluation of the colon showed no damage in control saline-treated animals without induction of colitis (Figure 1, Table 1, and Figure 2(a)). No colonic damage was also seen in animals without induction of colitis and treated with Mutaflor, rifaximin, or a combination thereof (Figure 1, Table 1). Rectal enema with acetic acid solution caused induction of colitis in all rats subjected to this procedure. In saline-treated rats, 7 days after the induction of colitis, the area of mucosal damage reached a value of 11.5 ± 0.6 mm2. Microscopic examination of the colon showed the presence of large lesions reaching the level of muscular membrane or even serous membrane (Table 1, Figure 2(b)). This alteration was associated with moderate or heavy inflammatory infiltration and the presence of mild fibrosis.


Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats.

Dembiński A, Warzecha Z, Ceranowicz P, Dembiński M, Cieszkowski J, Gosiewski T, Bulanda M, Kuśnierz-Cabala B, Gałązka K, Konturek PC - Gastroenterol Res Pract (2016)

Histological features of the rat colonic mucosa stained by haematoxylin and eosin (original magnification 400x). (a) Control rats without induction of colitis and treated with saline for 7 days; (b) rats with colitis treated with saline for 7 days; (c) rats with colitis treated with Mutaflor for 7 days; (d) rats with colitis treated with rifaximin for 7 days; and (e) rats with colitis treated with the combination of rifaximin plus Mutaflor for 7 days.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4940557&req=5

fig2: Histological features of the rat colonic mucosa stained by haematoxylin and eosin (original magnification 400x). (a) Control rats without induction of colitis and treated with saline for 7 days; (b) rats with colitis treated with saline for 7 days; (c) rats with colitis treated with Mutaflor for 7 days; (d) rats with colitis treated with rifaximin for 7 days; and (e) rats with colitis treated with the combination of rifaximin plus Mutaflor for 7 days.
Mentions: Macroscopic and microscopic evaluation of the colon showed no damage in control saline-treated animals without induction of colitis (Figure 1, Table 1, and Figure 2(a)). No colonic damage was also seen in animals without induction of colitis and treated with Mutaflor, rifaximin, or a combination thereof (Figure 1, Table 1). Rectal enema with acetic acid solution caused induction of colitis in all rats subjected to this procedure. In saline-treated rats, 7 days after the induction of colitis, the area of mucosal damage reached a value of 11.5 ± 0.6 mm2. Microscopic examination of the colon showed the presence of large lesions reaching the level of muscular membrane or even serous membrane (Table 1, Figure 2(b)). This alteration was associated with moderate or heavy inflammatory infiltration and the presence of mild fibrosis.

Bottom Line: This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis.In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.

ABSTRACT
Background. Inflammatory bowel disease results from the dysregulation of immune response to environmental and microbial agents in genetically susceptible individuals. The aim of the present study was to examine the effect of rifaximin and/or Mutaflor (Escherichia coli Nissle 1917, EcN) administration on the healing of acetic acid-induced colitis. Methods. Colitis was induced in male Wistar rats by rectal enema with 3.5% acetic acid solution. Rifaximin (50 mg/kg/dose) and/or Mutaflor (10(9) CFU/dose) were given intragastrically once a day. The severity of colitis was assessed at the 8th day after induction of inflammation. Results. Treatment with rifaximin significantly accelerated the healing of colonic damage. This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis. Moreover, administration of rifaximin significantly reduced concentration of proinflammatory TNF-α and activity of myeloperoxidase in colonic mucosa. Mutaflor given alone was without significant effect on activity of colitis. In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin. Moreover, Mutaflor led to settle of the colon by EcN and this effect was augmented by pretreatment with rifaximin. Conclusion. Rifaximin and Mutaflor exhibit synergic anti-inflammatory and therapeutic effect in acetic acid-induced colitis in rats.

No MeSH data available.


Related in: MedlinePlus