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Constituents of French Marigold (Tagetes patula L.) Flowers Protect Jurkat T-Cells against Oxidative Stress.

Chkhikvishvili I, Sanikidze T, Gogia N, Enukidze M, Machavariani M, Kipiani N, Vinokur Y, Rodov V - Oxid Med Cell Longev (2016)

Bottom Line: Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential.T. patula compounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells.Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Avenue, 0177 Tbilisi, Georgia.

ABSTRACT
The flowers of French marigold (Tagetes patula L.) are widely used in folk medicine, in particular for treating inflammation-related disorders. However, cellular mechanisms of this activity demand further investigation. In the present work, we studied the potential of T. patula compounds to alleviate the oxidative stress in hydrogen peroxide-challenged human lymphoblastoid Jurkat T-cells. Crude extracts of marigold flowers and purified fractions containing flavonoids patuletin, quercetagetin, and quercetin and their derivatives, as well as the carotenoid lutein, were brought in contact with Jurkat cells challenged with 25 or 50 μM H2O2. Hydrogen peroxide caused oxidative stress in the cells, manifested as generation of superoxide and peroxyl radicals, reduced viability, arrested cell cycle, and enhanced apoptosis. The stress was alleviated by marigold ingredients that demonstrated high radical-scavenging capacity and enhanced the activity of antioxidant enzymes involved in neutralization of reactive oxygen species. Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential. T. patula compounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells. Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

No MeSH data available.


Related in: MedlinePlus

Effect of hydrogen peroxide and French marigold fractions on the level of interleukin-10 (IL-10) in the Jurkat cells. Error bars represent standard deviations of five replications. Values marked with asterisks were significantly different from the nontreated control according to Student's t-test at P values of ≤0.01 and ≤0.05 designated as ∗∗ and ∗, respectively.
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fig9: Effect of hydrogen peroxide and French marigold fractions on the level of interleukin-10 (IL-10) in the Jurkat cells. Error bars represent standard deviations of five replications. Values marked with asterisks were significantly different from the nontreated control according to Student's t-test at P values of ≤0.01 and ≤0.05 designated as ∗∗ and ∗, respectively.

Mentions: Anti-inflammatory and antioxidant interleukin-10 (IL-10) increased in the Jurkat cells subjected to H2O2 challenge, most probably as a part of a defense mechanism against oxidative stress. Similar response was observed in cultured human keratinocytes exposed to ultraviolet irradiation [41]. Flavonoid fractions of T. patula further enhanced the IL-10 level in the H2O2-challenged Jurkat cells (Figure 9), similar to our previous work with Satureja hortensis [42]. The IL-10 is known to inhibit the apoptotic death of T-cells, presumably through upregulation of Bcl-2 [26]. This mechanism might underlay the alleviation of apoptosis observed in our study. On a whole organism level, the diet rich in phenolic antioxidants enhanced the IL-10 production in the animals enduring proinflammatory conditions, resulting in a decrease in proinflammatory factors, inhibited lipid peroxidation, increased HDL levels, and alleviated inflammation [43].


Constituents of French Marigold (Tagetes patula L.) Flowers Protect Jurkat T-Cells against Oxidative Stress.

Chkhikvishvili I, Sanikidze T, Gogia N, Enukidze M, Machavariani M, Kipiani N, Vinokur Y, Rodov V - Oxid Med Cell Longev (2016)

Effect of hydrogen peroxide and French marigold fractions on the level of interleukin-10 (IL-10) in the Jurkat cells. Error bars represent standard deviations of five replications. Values marked with asterisks were significantly different from the nontreated control according to Student's t-test at P values of ≤0.01 and ≤0.05 designated as ∗∗ and ∗, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940552&req=5

fig9: Effect of hydrogen peroxide and French marigold fractions on the level of interleukin-10 (IL-10) in the Jurkat cells. Error bars represent standard deviations of five replications. Values marked with asterisks were significantly different from the nontreated control according to Student's t-test at P values of ≤0.01 and ≤0.05 designated as ∗∗ and ∗, respectively.
Mentions: Anti-inflammatory and antioxidant interleukin-10 (IL-10) increased in the Jurkat cells subjected to H2O2 challenge, most probably as a part of a defense mechanism against oxidative stress. Similar response was observed in cultured human keratinocytes exposed to ultraviolet irradiation [41]. Flavonoid fractions of T. patula further enhanced the IL-10 level in the H2O2-challenged Jurkat cells (Figure 9), similar to our previous work with Satureja hortensis [42]. The IL-10 is known to inhibit the apoptotic death of T-cells, presumably through upregulation of Bcl-2 [26]. This mechanism might underlay the alleviation of apoptosis observed in our study. On a whole organism level, the diet rich in phenolic antioxidants enhanced the IL-10 production in the animals enduring proinflammatory conditions, resulting in a decrease in proinflammatory factors, inhibited lipid peroxidation, increased HDL levels, and alleviated inflammation [43].

Bottom Line: Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential.T. patula compounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells.Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Avenue, 0177 Tbilisi, Georgia.

ABSTRACT
The flowers of French marigold (Tagetes patula L.) are widely used in folk medicine, in particular for treating inflammation-related disorders. However, cellular mechanisms of this activity demand further investigation. In the present work, we studied the potential of T. patula compounds to alleviate the oxidative stress in hydrogen peroxide-challenged human lymphoblastoid Jurkat T-cells. Crude extracts of marigold flowers and purified fractions containing flavonoids patuletin, quercetagetin, and quercetin and their derivatives, as well as the carotenoid lutein, were brought in contact with Jurkat cells challenged with 25 or 50 μM H2O2. Hydrogen peroxide caused oxidative stress in the cells, manifested as generation of superoxide and peroxyl radicals, reduced viability, arrested cell cycle, and enhanced apoptosis. The stress was alleviated by marigold ingredients that demonstrated high radical-scavenging capacity and enhanced the activity of antioxidant enzymes involved in neutralization of reactive oxygen species. Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential. T. patula compounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells. Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

No MeSH data available.


Related in: MedlinePlus