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Constituents of French Marigold (Tagetes patula L.) Flowers Protect Jurkat T-Cells against Oxidative Stress.

Chkhikvishvili I, Sanikidze T, Gogia N, Enukidze M, Machavariani M, Kipiani N, Vinokur Y, Rodov V - Oxid Med Cell Longev (2016)

Bottom Line: Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential.T. patula compounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells.Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Avenue, 0177 Tbilisi, Georgia.

ABSTRACT
The flowers of French marigold (Tagetes patula L.) are widely used in folk medicine, in particular for treating inflammation-related disorders. However, cellular mechanisms of this activity demand further investigation. In the present work, we studied the potential of T. patula compounds to alleviate the oxidative stress in hydrogen peroxide-challenged human lymphoblastoid Jurkat T-cells. Crude extracts of marigold flowers and purified fractions containing flavonoids patuletin, quercetagetin, and quercetin and their derivatives, as well as the carotenoid lutein, were brought in contact with Jurkat cells challenged with 25 or 50 μM H2O2. Hydrogen peroxide caused oxidative stress in the cells, manifested as generation of superoxide and peroxyl radicals, reduced viability, arrested cell cycle, and enhanced apoptosis. The stress was alleviated by marigold ingredients that demonstrated high radical-scavenging capacity and enhanced the activity of antioxidant enzymes involved in neutralization of reactive oxygen species. Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential. T. patula compounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells. Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

No MeSH data available.


Related in: MedlinePlus

Effects of hydrogen peroxide and of the ethanolic French marigold extract on the percentage of cell cycle phase distributions of Jurkat cells (results of flow cytometry of propidium iodide-stained cell populations). Values marked with asterisks were significantly different from the respective cell cycle phase percentage in the nontreated control according to Student's t-test at P values of ≤0.01 and ≤0.05 designated as ∗∗ and ∗, respectively.
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fig6: Effects of hydrogen peroxide and of the ethanolic French marigold extract on the percentage of cell cycle phase distributions of Jurkat cells (results of flow cytometry of propidium iodide-stained cell populations). Values marked with asterisks were significantly different from the respective cell cycle phase percentage in the nontreated control according to Student's t-test at P values of ≤0.01 and ≤0.05 designated as ∗∗ and ∗, respectively.

Mentions: The H2O2-caused oxidative stress changed the cell cycle phase distribution, restricting DNA replication (phase S) and increasing the relative proportions of G0/G1 cells (the G0/G1 arrest) and apoptotic cells (Figure 6). The characteristic apoptotic changes (chromatin condensation, nuclear fragmentation, and cytoplasmic vacuolization) in typical H2O2-exposed Jurkat cells are presented in transmission electron microscope images (Figure 7). Adding the T. patula extract to the H2O2-challenged cells largely normalized their cell cycle (Figure 6). Similar trends were revealed by evaluating the percentage of apoptotic cells in the population (the apoptotic ratio) by flow cytometry on the basis of mitochondrial transmembrane potential measured by DiOC6 test. An upsurge in the apoptotic ratio was induced by hydrogen peroxide alone, while this increase was counteracted by coadministering the T. patula fractions containing patuletin, quercetagetin/quercetin, or lutein (Figure 8). Certain discrepancy in the proportion of apoptotic cells was evident between the two flow cytometry methods, most probably due to the different separation criteria used. Similarly, Özgen et al. [40] reported that in the T-cells, the DiOC6 technique gave higher estimation of apoptotic cell population as compared to the propidium iodide-based method.


Constituents of French Marigold (Tagetes patula L.) Flowers Protect Jurkat T-Cells against Oxidative Stress.

Chkhikvishvili I, Sanikidze T, Gogia N, Enukidze M, Machavariani M, Kipiani N, Vinokur Y, Rodov V - Oxid Med Cell Longev (2016)

Effects of hydrogen peroxide and of the ethanolic French marigold extract on the percentage of cell cycle phase distributions of Jurkat cells (results of flow cytometry of propidium iodide-stained cell populations). Values marked with asterisks were significantly different from the respective cell cycle phase percentage in the nontreated control according to Student's t-test at P values of ≤0.01 and ≤0.05 designated as ∗∗ and ∗, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940552&req=5

fig6: Effects of hydrogen peroxide and of the ethanolic French marigold extract on the percentage of cell cycle phase distributions of Jurkat cells (results of flow cytometry of propidium iodide-stained cell populations). Values marked with asterisks were significantly different from the respective cell cycle phase percentage in the nontreated control according to Student's t-test at P values of ≤0.01 and ≤0.05 designated as ∗∗ and ∗, respectively.
Mentions: The H2O2-caused oxidative stress changed the cell cycle phase distribution, restricting DNA replication (phase S) and increasing the relative proportions of G0/G1 cells (the G0/G1 arrest) and apoptotic cells (Figure 6). The characteristic apoptotic changes (chromatin condensation, nuclear fragmentation, and cytoplasmic vacuolization) in typical H2O2-exposed Jurkat cells are presented in transmission electron microscope images (Figure 7). Adding the T. patula extract to the H2O2-challenged cells largely normalized their cell cycle (Figure 6). Similar trends were revealed by evaluating the percentage of apoptotic cells in the population (the apoptotic ratio) by flow cytometry on the basis of mitochondrial transmembrane potential measured by DiOC6 test. An upsurge in the apoptotic ratio was induced by hydrogen peroxide alone, while this increase was counteracted by coadministering the T. patula fractions containing patuletin, quercetagetin/quercetin, or lutein (Figure 8). Certain discrepancy in the proportion of apoptotic cells was evident between the two flow cytometry methods, most probably due to the different separation criteria used. Similarly, Özgen et al. [40] reported that in the T-cells, the DiOC6 technique gave higher estimation of apoptotic cell population as compared to the propidium iodide-based method.

Bottom Line: Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential.T. patula compounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells.Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Avenue, 0177 Tbilisi, Georgia.

ABSTRACT
The flowers of French marigold (Tagetes patula L.) are widely used in folk medicine, in particular for treating inflammation-related disorders. However, cellular mechanisms of this activity demand further investigation. In the present work, we studied the potential of T. patula compounds to alleviate the oxidative stress in hydrogen peroxide-challenged human lymphoblastoid Jurkat T-cells. Crude extracts of marigold flowers and purified fractions containing flavonoids patuletin, quercetagetin, and quercetin and their derivatives, as well as the carotenoid lutein, were brought in contact with Jurkat cells challenged with 25 or 50 μM H2O2. Hydrogen peroxide caused oxidative stress in the cells, manifested as generation of superoxide and peroxyl radicals, reduced viability, arrested cell cycle, and enhanced apoptosis. The stress was alleviated by marigold ingredients that demonstrated high radical-scavenging capacity and enhanced the activity of antioxidant enzymes involved in neutralization of reactive oxygen species. Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential. T. patula compounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells. Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

No MeSH data available.


Related in: MedlinePlus