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Constituents of French Marigold (Tagetes patula L.) Flowers Protect Jurkat T-Cells against Oxidative Stress.

Chkhikvishvili I, Sanikidze T, Gogia N, Enukidze M, Machavariani M, Kipiani N, Vinokur Y, Rodov V - Oxid Med Cell Longev (2016)

Bottom Line: Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential.T. patula compounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells.Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Avenue, 0177 Tbilisi, Georgia.

ABSTRACT
The flowers of French marigold (Tagetes patula L.) are widely used in folk medicine, in particular for treating inflammation-related disorders. However, cellular mechanisms of this activity demand further investigation. In the present work, we studied the potential of T. patula compounds to alleviate the oxidative stress in hydrogen peroxide-challenged human lymphoblastoid Jurkat T-cells. Crude extracts of marigold flowers and purified fractions containing flavonoids patuletin, quercetagetin, and quercetin and their derivatives, as well as the carotenoid lutein, were brought in contact with Jurkat cells challenged with 25 or 50 μM H2O2. Hydrogen peroxide caused oxidative stress in the cells, manifested as generation of superoxide and peroxyl radicals, reduced viability, arrested cell cycle, and enhanced apoptosis. The stress was alleviated by marigold ingredients that demonstrated high radical-scavenging capacity and enhanced the activity of antioxidant enzymes involved in neutralization of reactive oxygen species. Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential. T. patula compounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells. Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

No MeSH data available.


Related in: MedlinePlus

Effects of French marigold extracts and purified fractions on the generation of superoxide (a) and peroxyl (b) radicals in Jurkat cells subjected to hydrogen peroxide-induced oxidative stress (EPR signal intensity, arbitrary units). Error bars represent standard deviations of five replications. Values marked with asterisks were significantly different from the control subjected to the same H2O2 concentration, according to Student's t-test at P values of ≤0.001, ≤0.01, and ≤0.05 designated as ∗∗∗, ∗∗, and ∗, respectively.
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fig3: Effects of French marigold extracts and purified fractions on the generation of superoxide (a) and peroxyl (b) radicals in Jurkat cells subjected to hydrogen peroxide-induced oxidative stress (EPR signal intensity, arbitrary units). Error bars represent standard deviations of five replications. Values marked with asterisks were significantly different from the control subjected to the same H2O2 concentration, according to Student's t-test at P values of ≤0.001, ≤0.01, and ≤0.05 designated as ∗∗∗, ∗∗, and ∗, respectively.

Mentions: The addition of 25 or 50 μM of hydrogen peroxide caused generation of superoxide and peroxyl radicals in the Jurkat culture evidenced by EPR spectroscopy (Figure 3), most probably through the mechanisms described by Petlicki and Van De Ven [25]. No radicals were detected in the absence of hydrogen peroxide (data not shown). Adding T. patula fractions significantly reduced the level of superoxide and peroxyl radicals in the H2O2-challenged Jurkat cultures, with purified patuletin being the most efficient radical scavenger.


Constituents of French Marigold (Tagetes patula L.) Flowers Protect Jurkat T-Cells against Oxidative Stress.

Chkhikvishvili I, Sanikidze T, Gogia N, Enukidze M, Machavariani M, Kipiani N, Vinokur Y, Rodov V - Oxid Med Cell Longev (2016)

Effects of French marigold extracts and purified fractions on the generation of superoxide (a) and peroxyl (b) radicals in Jurkat cells subjected to hydrogen peroxide-induced oxidative stress (EPR signal intensity, arbitrary units). Error bars represent standard deviations of five replications. Values marked with asterisks were significantly different from the control subjected to the same H2O2 concentration, according to Student's t-test at P values of ≤0.001, ≤0.01, and ≤0.05 designated as ∗∗∗, ∗∗, and ∗, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940552&req=5

fig3: Effects of French marigold extracts and purified fractions on the generation of superoxide (a) and peroxyl (b) radicals in Jurkat cells subjected to hydrogen peroxide-induced oxidative stress (EPR signal intensity, arbitrary units). Error bars represent standard deviations of five replications. Values marked with asterisks were significantly different from the control subjected to the same H2O2 concentration, according to Student's t-test at P values of ≤0.001, ≤0.01, and ≤0.05 designated as ∗∗∗, ∗∗, and ∗, respectively.
Mentions: The addition of 25 or 50 μM of hydrogen peroxide caused generation of superoxide and peroxyl radicals in the Jurkat culture evidenced by EPR spectroscopy (Figure 3), most probably through the mechanisms described by Petlicki and Van De Ven [25]. No radicals were detected in the absence of hydrogen peroxide (data not shown). Adding T. patula fractions significantly reduced the level of superoxide and peroxyl radicals in the H2O2-challenged Jurkat cultures, with purified patuletin being the most efficient radical scavenger.

Bottom Line: Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential.T. patula compounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells.Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Avenue, 0177 Tbilisi, Georgia.

ABSTRACT
The flowers of French marigold (Tagetes patula L.) are widely used in folk medicine, in particular for treating inflammation-related disorders. However, cellular mechanisms of this activity demand further investigation. In the present work, we studied the potential of T. patula compounds to alleviate the oxidative stress in hydrogen peroxide-challenged human lymphoblastoid Jurkat T-cells. Crude extracts of marigold flowers and purified fractions containing flavonoids patuletin, quercetagetin, and quercetin and their derivatives, as well as the carotenoid lutein, were brought in contact with Jurkat cells challenged with 25 or 50 μM H2O2. Hydrogen peroxide caused oxidative stress in the cells, manifested as generation of superoxide and peroxyl radicals, reduced viability, arrested cell cycle, and enhanced apoptosis. The stress was alleviated by marigold ingredients that demonstrated high radical-scavenging capacity and enhanced the activity of antioxidant enzymes involved in neutralization of reactive oxygen species. Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential. T. patula compounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells. Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

No MeSH data available.


Related in: MedlinePlus