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Sulforaphane Ameliorates Bladder Dysfunction through Activation of the Nrf2-ARE Pathway in a Rat Model of Partial Bladder Outlet Obstruction.

Liu C, Xu H, Fu S, Chen Y, Chen Q, Cai Z, Zhou J, Wang Z - Oxid Med Cell Longev (2016)

Bottom Line: SFN treatment also increased the activity of SOD, GSH-Px, and CAT compared to the other groups.Moreover, SFN could reduce the ratio of Bax/Bcl-2 expression.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

ABSTRACT
Purpose. We evaluated the effect of sulforaphane (SFN) treatment on the function and changes of expression of Nrf2-ARE pathway in the bladder of rats with bladder outlet obstruction (BOO). Materials and Methods. A total of 18 male Sprague-Dawley rats at age of 8 weeks were divided into 3 groups (6 of each): the sham operated group, the BOO group, and the BOO+SFN group. We examined histological alterations and the changes of oxidative stress markers and the protein expression of the Nrf2-ARE pathway. Results. We found that SFN treatment could prolong micturition interval and increase bladder capacity and bladder compliance. However, the peak voiding pressure was lower than BOO group. SFN treatment can ameliorate the increase of collagen fibers induced by obstruction. SFN treatment also increased the activity of SOD, GSH-Px, and CAT compared to the other groups. The level of bladder cell apoptosis was decreased in BOO rats with SFN treatment. Moreover, SFN could reduce the ratio of Bax/Bcl-2 expression. Furthermore, SFN could activate the Nrf2 expression with elevation of its target antioxidant proteins. Conclusions. The sulforaphane-mediated decrease of oxidative stress and activation of the Nrf2-ARE pathway may ameliorate bladder dysfunction caused by bladder outlet obstruction.

No MeSH data available.


Related in: MedlinePlus

Effect of SFN on cell apoptosis and proliferation in BOO rats. (a) TUNEL staining showing the cell apoptosis level of the bladder in the three groups. Original magnification ×400. (b) The protein expression of Bax/Bcl2 ratio in the bladder of the three groups. (c) The statistical results of TUNEL staining in the three groups. ∗n = 6, P < 0.001 versus sham group. #n = 6, P < 0.001 versus BOO group. (d) The statistical results of protein expression of Bax/Bcl2 ratio in the bladder of the three groups. ∗P < 0.05 versus sham group. #P < 0.05 versus BOO group. (e) The expression of PCNA by immunohistochemical staining in the three groups. Original magnification ×200.
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fig4: Effect of SFN on cell apoptosis and proliferation in BOO rats. (a) TUNEL staining showing the cell apoptosis level of the bladder in the three groups. Original magnification ×400. (b) The protein expression of Bax/Bcl2 ratio in the bladder of the three groups. (c) The statistical results of TUNEL staining in the three groups. ∗n = 6, P < 0.001 versus sham group. #n = 6, P < 0.001 versus BOO group. (d) The statistical results of protein expression of Bax/Bcl2 ratio in the bladder of the three groups. ∗P < 0.05 versus sham group. #P < 0.05 versus BOO group. (e) The expression of PCNA by immunohistochemical staining in the three groups. Original magnification ×200.

Mentions: TUNEL staining was measured to observe whether SFN had protective effects on the cell apoptosis level of bladder (Figures 4(a) and 4(c)). The number of apoptotic cells in the bladder of BOO rats was markedly increased compared to sham rats (P < 0.001), whereas SFN treatment decreased the number of apoptotic cell in the bladder of BOO rats (P = 0.001). In accordance with the result of TUNEL staining, western blotting showed that the Bax/Bcl2 expression ratio was significantly increased in the bladder of BOO rats; however, this ratio was decreased in BOO+SFN rats (Figures 4(b) and 4(d)). Moreover, the immunohistochemical staining of PCNA showed that SFN did not cause obvious increase on cell proliferation in BOO+SFN group compared to BOO group (Figure 4(e)).


Sulforaphane Ameliorates Bladder Dysfunction through Activation of the Nrf2-ARE Pathway in a Rat Model of Partial Bladder Outlet Obstruction.

Liu C, Xu H, Fu S, Chen Y, Chen Q, Cai Z, Zhou J, Wang Z - Oxid Med Cell Longev (2016)

Effect of SFN on cell apoptosis and proliferation in BOO rats. (a) TUNEL staining showing the cell apoptosis level of the bladder in the three groups. Original magnification ×400. (b) The protein expression of Bax/Bcl2 ratio in the bladder of the three groups. (c) The statistical results of TUNEL staining in the three groups. ∗n = 6, P < 0.001 versus sham group. #n = 6, P < 0.001 versus BOO group. (d) The statistical results of protein expression of Bax/Bcl2 ratio in the bladder of the three groups. ∗P < 0.05 versus sham group. #P < 0.05 versus BOO group. (e) The expression of PCNA by immunohistochemical staining in the three groups. Original magnification ×200.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig4: Effect of SFN on cell apoptosis and proliferation in BOO rats. (a) TUNEL staining showing the cell apoptosis level of the bladder in the three groups. Original magnification ×400. (b) The protein expression of Bax/Bcl2 ratio in the bladder of the three groups. (c) The statistical results of TUNEL staining in the three groups. ∗n = 6, P < 0.001 versus sham group. #n = 6, P < 0.001 versus BOO group. (d) The statistical results of protein expression of Bax/Bcl2 ratio in the bladder of the three groups. ∗P < 0.05 versus sham group. #P < 0.05 versus BOO group. (e) The expression of PCNA by immunohistochemical staining in the three groups. Original magnification ×200.
Mentions: TUNEL staining was measured to observe whether SFN had protective effects on the cell apoptosis level of bladder (Figures 4(a) and 4(c)). The number of apoptotic cells in the bladder of BOO rats was markedly increased compared to sham rats (P < 0.001), whereas SFN treatment decreased the number of apoptotic cell in the bladder of BOO rats (P = 0.001). In accordance with the result of TUNEL staining, western blotting showed that the Bax/Bcl2 expression ratio was significantly increased in the bladder of BOO rats; however, this ratio was decreased in BOO+SFN rats (Figures 4(b) and 4(d)). Moreover, the immunohistochemical staining of PCNA showed that SFN did not cause obvious increase on cell proliferation in BOO+SFN group compared to BOO group (Figure 4(e)).

Bottom Line: SFN treatment also increased the activity of SOD, GSH-Px, and CAT compared to the other groups.Moreover, SFN could reduce the ratio of Bax/Bcl-2 expression.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

ABSTRACT
Purpose. We evaluated the effect of sulforaphane (SFN) treatment on the function and changes of expression of Nrf2-ARE pathway in the bladder of rats with bladder outlet obstruction (BOO). Materials and Methods. A total of 18 male Sprague-Dawley rats at age of 8 weeks were divided into 3 groups (6 of each): the sham operated group, the BOO group, and the BOO+SFN group. We examined histological alterations and the changes of oxidative stress markers and the protein expression of the Nrf2-ARE pathway. Results. We found that SFN treatment could prolong micturition interval and increase bladder capacity and bladder compliance. However, the peak voiding pressure was lower than BOO group. SFN treatment can ameliorate the increase of collagen fibers induced by obstruction. SFN treatment also increased the activity of SOD, GSH-Px, and CAT compared to the other groups. The level of bladder cell apoptosis was decreased in BOO rats with SFN treatment. Moreover, SFN could reduce the ratio of Bax/Bcl-2 expression. Furthermore, SFN could activate the Nrf2 expression with elevation of its target antioxidant proteins. Conclusions. The sulforaphane-mediated decrease of oxidative stress and activation of the Nrf2-ARE pathway may ameliorate bladder dysfunction caused by bladder outlet obstruction.

No MeSH data available.


Related in: MedlinePlus