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Sulforaphane Ameliorates Bladder Dysfunction through Activation of the Nrf2-ARE Pathway in a Rat Model of Partial Bladder Outlet Obstruction.

Liu C, Xu H, Fu S, Chen Y, Chen Q, Cai Z, Zhou J, Wang Z - Oxid Med Cell Longev (2016)

Bottom Line: SFN treatment also increased the activity of SOD, GSH-Px, and CAT compared to the other groups.Moreover, SFN could reduce the ratio of Bax/Bcl-2 expression.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

ABSTRACT
Purpose. We evaluated the effect of sulforaphane (SFN) treatment on the function and changes of expression of Nrf2-ARE pathway in the bladder of rats with bladder outlet obstruction (BOO). Materials and Methods. A total of 18 male Sprague-Dawley rats at age of 8 weeks were divided into 3 groups (6 of each): the sham operated group, the BOO group, and the BOO+SFN group. We examined histological alterations and the changes of oxidative stress markers and the protein expression of the Nrf2-ARE pathway. Results. We found that SFN treatment could prolong micturition interval and increase bladder capacity and bladder compliance. However, the peak voiding pressure was lower than BOO group. SFN treatment can ameliorate the increase of collagen fibers induced by obstruction. SFN treatment also increased the activity of SOD, GSH-Px, and CAT compared to the other groups. The level of bladder cell apoptosis was decreased in BOO rats with SFN treatment. Moreover, SFN could reduce the ratio of Bax/Bcl-2 expression. Furthermore, SFN could activate the Nrf2 expression with elevation of its target antioxidant proteins. Conclusions. The sulforaphane-mediated decrease of oxidative stress and activation of the Nrf2-ARE pathway may ameliorate bladder dysfunction caused by bladder outlet obstruction.

No MeSH data available.


Related in: MedlinePlus

Effects of SFN on attenuating oxidative stress in BOO rats. (a) MDA level in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group. #n = 6, P < 0.05 versus BOO group. (b) MDA level in serum of the three groups, ∗n = 5, P < 0.05 versus sham group. (c) The activity of total SOD in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group. #n = 6, P < 0.05 versus BOO group. (d) The activity of GSH-Px in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group. #n = 6, P < 0.05 versus BOO group. (e) The activity of CAT in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group.
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fig3: Effects of SFN on attenuating oxidative stress in BOO rats. (a) MDA level in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group. #n = 6, P < 0.05 versus BOO group. (b) MDA level in serum of the three groups, ∗n = 5, P < 0.05 versus sham group. (c) The activity of total SOD in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group. #n = 6, P < 0.05 versus BOO group. (d) The activity of GSH-Px in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group. #n = 6, P < 0.05 versus BOO group. (e) The activity of CAT in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group.

Mentions: The level of MDA, total SOD, GSH-Px, and CAT were measured to evaluate the level of oxidative stress. Our results showed that the content of MDA in bladder was significantly increased in BOO rats compared to sham rats (2.32 ± 0.33 versus 1.26 ± 0.24). However, the level of MDA was decreased in BOO+SFN rats (1.31 ± 0.49) (Figure 3(a)). The activities of total SOD, GSH-Px, and CAT were significantly decreased in BOO rats compared to sham rats (41.75 ± 13.59 versus 126.73 ± 20.31; 164.46 ± 20.64 versus 225.42 ± 30.65; 2.80 ± 0.37 versus 4.56 ± 0.93), respectively. However, treatment with SFN in BOO rats could significantly increase the total SOD, GSH-Px, and CAT activities (Figures 3(c), 3(d), and 3(e)). The MDA level in serum was also significantly elevated in BOO rats (Figure 3(b)).


Sulforaphane Ameliorates Bladder Dysfunction through Activation of the Nrf2-ARE Pathway in a Rat Model of Partial Bladder Outlet Obstruction.

Liu C, Xu H, Fu S, Chen Y, Chen Q, Cai Z, Zhou J, Wang Z - Oxid Med Cell Longev (2016)

Effects of SFN on attenuating oxidative stress in BOO rats. (a) MDA level in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group. #n = 6, P < 0.05 versus BOO group. (b) MDA level in serum of the three groups, ∗n = 5, P < 0.05 versus sham group. (c) The activity of total SOD in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group. #n = 6, P < 0.05 versus BOO group. (d) The activity of GSH-Px in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group. #n = 6, P < 0.05 versus BOO group. (e) The activity of CAT in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig3: Effects of SFN on attenuating oxidative stress in BOO rats. (a) MDA level in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group. #n = 6, P < 0.05 versus BOO group. (b) MDA level in serum of the three groups, ∗n = 5, P < 0.05 versus sham group. (c) The activity of total SOD in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group. #n = 6, P < 0.05 versus BOO group. (d) The activity of GSH-Px in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group. #n = 6, P < 0.05 versus BOO group. (e) The activity of CAT in the bladder of the three groups, ∗n = 6, P < 0.05 versus sham group.
Mentions: The level of MDA, total SOD, GSH-Px, and CAT were measured to evaluate the level of oxidative stress. Our results showed that the content of MDA in bladder was significantly increased in BOO rats compared to sham rats (2.32 ± 0.33 versus 1.26 ± 0.24). However, the level of MDA was decreased in BOO+SFN rats (1.31 ± 0.49) (Figure 3(a)). The activities of total SOD, GSH-Px, and CAT were significantly decreased in BOO rats compared to sham rats (41.75 ± 13.59 versus 126.73 ± 20.31; 164.46 ± 20.64 versus 225.42 ± 30.65; 2.80 ± 0.37 versus 4.56 ± 0.93), respectively. However, treatment with SFN in BOO rats could significantly increase the total SOD, GSH-Px, and CAT activities (Figures 3(c), 3(d), and 3(e)). The MDA level in serum was also significantly elevated in BOO rats (Figure 3(b)).

Bottom Line: SFN treatment also increased the activity of SOD, GSH-Px, and CAT compared to the other groups.Moreover, SFN could reduce the ratio of Bax/Bcl-2 expression.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

ABSTRACT
Purpose. We evaluated the effect of sulforaphane (SFN) treatment on the function and changes of expression of Nrf2-ARE pathway in the bladder of rats with bladder outlet obstruction (BOO). Materials and Methods. A total of 18 male Sprague-Dawley rats at age of 8 weeks were divided into 3 groups (6 of each): the sham operated group, the BOO group, and the BOO+SFN group. We examined histological alterations and the changes of oxidative stress markers and the protein expression of the Nrf2-ARE pathway. Results. We found that SFN treatment could prolong micturition interval and increase bladder capacity and bladder compliance. However, the peak voiding pressure was lower than BOO group. SFN treatment can ameliorate the increase of collagen fibers induced by obstruction. SFN treatment also increased the activity of SOD, GSH-Px, and CAT compared to the other groups. The level of bladder cell apoptosis was decreased in BOO rats with SFN treatment. Moreover, SFN could reduce the ratio of Bax/Bcl-2 expression. Furthermore, SFN could activate the Nrf2 expression with elevation of its target antioxidant proteins. Conclusions. The sulforaphane-mediated decrease of oxidative stress and activation of the Nrf2-ARE pathway may ameliorate bladder dysfunction caused by bladder outlet obstruction.

No MeSH data available.


Related in: MedlinePlus