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Sulforaphane Ameliorates Bladder Dysfunction through Activation of the Nrf2-ARE Pathway in a Rat Model of Partial Bladder Outlet Obstruction.

Liu C, Xu H, Fu S, Chen Y, Chen Q, Cai Z, Zhou J, Wang Z - Oxid Med Cell Longev (2016)

Bottom Line: SFN treatment also increased the activity of SOD, GSH-Px, and CAT compared to the other groups.Moreover, SFN could reduce the ratio of Bax/Bcl-2 expression.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

ABSTRACT
Purpose. We evaluated the effect of sulforaphane (SFN) treatment on the function and changes of expression of Nrf2-ARE pathway in the bladder of rats with bladder outlet obstruction (BOO). Materials and Methods. A total of 18 male Sprague-Dawley rats at age of 8 weeks were divided into 3 groups (6 of each): the sham operated group, the BOO group, and the BOO+SFN group. We examined histological alterations and the changes of oxidative stress markers and the protein expression of the Nrf2-ARE pathway. Results. We found that SFN treatment could prolong micturition interval and increase bladder capacity and bladder compliance. However, the peak voiding pressure was lower than BOO group. SFN treatment can ameliorate the increase of collagen fibers induced by obstruction. SFN treatment also increased the activity of SOD, GSH-Px, and CAT compared to the other groups. The level of bladder cell apoptosis was decreased in BOO rats with SFN treatment. Moreover, SFN could reduce the ratio of Bax/Bcl-2 expression. Furthermore, SFN could activate the Nrf2 expression with elevation of its target antioxidant proteins. Conclusions. The sulforaphane-mediated decrease of oxidative stress and activation of the Nrf2-ARE pathway may ameliorate bladder dysfunction caused by bladder outlet obstruction.

No MeSH data available.


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Effect of SFN on bladder histological changes in BOO rats. Original magnification ×100. (a) HE staining in sham, BOO, and BOO+SFN bladders; (b) Masson trichrome staining in sham, BOO, and BOO+SFN bladders; (c) the percentage of collagen fibers in muscular layer in sham, BOO, and BOO+SFN bladders, ∗n = 6, P < 0.05 versus sham group, #n = 6, P < 0.05 versus BOO group.
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fig2: Effect of SFN on bladder histological changes in BOO rats. Original magnification ×100. (a) HE staining in sham, BOO, and BOO+SFN bladders; (b) Masson trichrome staining in sham, BOO, and BOO+SFN bladders; (c) the percentage of collagen fibers in muscular layer in sham, BOO, and BOO+SFN bladders, ∗n = 6, P < 0.05 versus sham group, #n = 6, P < 0.05 versus BOO group.

Mentions: HE staining showed that SFN had some protective effect on BOO bladder (Figure 2(a)). BOO caused obvious histological changes, such as the structural damage of detrusor smooth muscle. However, treatment with SFN significantly alleviated these histological changes in the bladders of BOO rats. The area ratio of collagen fibers was 43.55 ± 0.86, 48.40 ± 2.25, and 44.13 ± 1.64 in sham group, BOO group, and BOO+SFN group, respectively. An increase of collagen fibers in the muscular layer was observed in the BOO group and this increase was suppressed in the BOO+SFN group (Figures 2(b) and 2(c)).


Sulforaphane Ameliorates Bladder Dysfunction through Activation of the Nrf2-ARE Pathway in a Rat Model of Partial Bladder Outlet Obstruction.

Liu C, Xu H, Fu S, Chen Y, Chen Q, Cai Z, Zhou J, Wang Z - Oxid Med Cell Longev (2016)

Effect of SFN on bladder histological changes in BOO rats. Original magnification ×100. (a) HE staining in sham, BOO, and BOO+SFN bladders; (b) Masson trichrome staining in sham, BOO, and BOO+SFN bladders; (c) the percentage of collagen fibers in muscular layer in sham, BOO, and BOO+SFN bladders, ∗n = 6, P < 0.05 versus sham group, #n = 6, P < 0.05 versus BOO group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4940551&req=5

fig2: Effect of SFN on bladder histological changes in BOO rats. Original magnification ×100. (a) HE staining in sham, BOO, and BOO+SFN bladders; (b) Masson trichrome staining in sham, BOO, and BOO+SFN bladders; (c) the percentage of collagen fibers in muscular layer in sham, BOO, and BOO+SFN bladders, ∗n = 6, P < 0.05 versus sham group, #n = 6, P < 0.05 versus BOO group.
Mentions: HE staining showed that SFN had some protective effect on BOO bladder (Figure 2(a)). BOO caused obvious histological changes, such as the structural damage of detrusor smooth muscle. However, treatment with SFN significantly alleviated these histological changes in the bladders of BOO rats. The area ratio of collagen fibers was 43.55 ± 0.86, 48.40 ± 2.25, and 44.13 ± 1.64 in sham group, BOO group, and BOO+SFN group, respectively. An increase of collagen fibers in the muscular layer was observed in the BOO group and this increase was suppressed in the BOO+SFN group (Figures 2(b) and 2(c)).

Bottom Line: SFN treatment also increased the activity of SOD, GSH-Px, and CAT compared to the other groups.Moreover, SFN could reduce the ratio of Bax/Bcl-2 expression.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

ABSTRACT
Purpose. We evaluated the effect of sulforaphane (SFN) treatment on the function and changes of expression of Nrf2-ARE pathway in the bladder of rats with bladder outlet obstruction (BOO). Materials and Methods. A total of 18 male Sprague-Dawley rats at age of 8 weeks were divided into 3 groups (6 of each): the sham operated group, the BOO group, and the BOO+SFN group. We examined histological alterations and the changes of oxidative stress markers and the protein expression of the Nrf2-ARE pathway. Results. We found that SFN treatment could prolong micturition interval and increase bladder capacity and bladder compliance. However, the peak voiding pressure was lower than BOO group. SFN treatment can ameliorate the increase of collagen fibers induced by obstruction. SFN treatment also increased the activity of SOD, GSH-Px, and CAT compared to the other groups. The level of bladder cell apoptosis was decreased in BOO rats with SFN treatment. Moreover, SFN could reduce the ratio of Bax/Bcl-2 expression. Furthermore, SFN could activate the Nrf2 expression with elevation of its target antioxidant proteins. Conclusions. The sulforaphane-mediated decrease of oxidative stress and activation of the Nrf2-ARE pathway may ameliorate bladder dysfunction caused by bladder outlet obstruction.

No MeSH data available.


Related in: MedlinePlus