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Geographical Distribution and New Situation of Leishmania Species after the Control of Cutaneous Leishmaniasis Foci in Errachidia Province, Morocco, in 2014.

Mohamed Mahmoud el A, Faiza S, Lemine M, Smaine C, Adlaoui el B, Khalid H, Abderrahim S, Hajiba F - Biomed Res Int (2016)

Bottom Line: The number of cases declined sharply and decreased from 3445 cases in 2010 to 8 cases in 2014 following the control action plan interventions.This is the first molecular identification of L. tropica in Errachidia province.The detection of this species after the intensified control measures strategies suggests that it was probably dissipated through the dominance of L. major.

View Article: PubMed Central - PubMed

Affiliation: National Reference Laboratory of Leishmaniasis, National Institute of Hygiene, 27 Avenue Ibn Battuta, Agdal, 11400 Rabat, Morocco; Laboratory of Zoology and General Biology, Faculty of Sciences, Mohammed V University in Rabat, Avenue Ibn Battouta, BP 1014, 10000 Rabat, Morocco.

ABSTRACT
In Errachidia province, the incidence of cutaneous leishmaniasis (CL) has increased over the past decade and it was higher in 2010 (860.34 per 100,000 inhabitants), with 3445 cases. The number of cases declined sharply and decreased from 3445 cases in 2010 to 8 cases in 2014 following the control action plan interventions. The total of patients was diagnosed only on clinical basis and the lesions were considered caused by L. major. The epidemiological study was conducted between 2001 and 2014 and the molecular detection of CL was studied to identify the circulating parasite species in this province by using the ITS1-PCR-RFLP methods. In 2010, the molecular identification of 11 samples revealed the presence of L. major in the most affected circles: Goulmima, Er-Rissani, and Errachidia. In 2014 the molecular characterization of 7 among 8 cases reported in this year showed the presence of L. tropica in Errachidia circle. This is the first molecular identification of L. tropica in Errachidia province. The detection of this species after the intensified control measures strategies suggests that it was probably dissipated through the dominance of L. major.

No MeSH data available.


Related in: MedlinePlus

Agarose gel electrophoresis analysis of ITS1-PCR-RFLP amplified products from different slides collected, lanes 1–4 (L. major detected in the samples 2010), and lanes 5–11 (L. tropica detected in the samples 2014), C: negative control, Lt: L. tropica, Lm: L. major, Li: L. infantum, and WM: weight marker (50 pb).
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fig7: Agarose gel electrophoresis analysis of ITS1-PCR-RFLP amplified products from different slides collected, lanes 1–4 (L. major detected in the samples 2010), and lanes 5–11 (L. tropica detected in the samples 2014), C: negative control, Lt: L. tropica, Lm: L. major, Li: L. infantum, and WM: weight marker (50 pb).

Mentions: The results of the molecular study of 11 samples collected in 2010 showed that CL was due to L. major in Errachidia, Er-Rissani, and Goulmima circles. In 2014, the molecular identification of 7 among the 8 cases reported in the Errachidia circles showed the presence of only L. tropica (Table 1, Figures 2 and 7).


Geographical Distribution and New Situation of Leishmania Species after the Control of Cutaneous Leishmaniasis Foci in Errachidia Province, Morocco, in 2014.

Mohamed Mahmoud el A, Faiza S, Lemine M, Smaine C, Adlaoui el B, Khalid H, Abderrahim S, Hajiba F - Biomed Res Int (2016)

Agarose gel electrophoresis analysis of ITS1-PCR-RFLP amplified products from different slides collected, lanes 1–4 (L. major detected in the samples 2010), and lanes 5–11 (L. tropica detected in the samples 2014), C: negative control, Lt: L. tropica, Lm: L. major, Li: L. infantum, and WM: weight marker (50 pb).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940537&req=5

fig7: Agarose gel electrophoresis analysis of ITS1-PCR-RFLP amplified products from different slides collected, lanes 1–4 (L. major detected in the samples 2010), and lanes 5–11 (L. tropica detected in the samples 2014), C: negative control, Lt: L. tropica, Lm: L. major, Li: L. infantum, and WM: weight marker (50 pb).
Mentions: The results of the molecular study of 11 samples collected in 2010 showed that CL was due to L. major in Errachidia, Er-Rissani, and Goulmima circles. In 2014, the molecular identification of 7 among the 8 cases reported in the Errachidia circles showed the presence of only L. tropica (Table 1, Figures 2 and 7).

Bottom Line: The number of cases declined sharply and decreased from 3445 cases in 2010 to 8 cases in 2014 following the control action plan interventions.This is the first molecular identification of L. tropica in Errachidia province.The detection of this species after the intensified control measures strategies suggests that it was probably dissipated through the dominance of L. major.

View Article: PubMed Central - PubMed

Affiliation: National Reference Laboratory of Leishmaniasis, National Institute of Hygiene, 27 Avenue Ibn Battuta, Agdal, 11400 Rabat, Morocco; Laboratory of Zoology and General Biology, Faculty of Sciences, Mohammed V University in Rabat, Avenue Ibn Battouta, BP 1014, 10000 Rabat, Morocco.

ABSTRACT
In Errachidia province, the incidence of cutaneous leishmaniasis (CL) has increased over the past decade and it was higher in 2010 (860.34 per 100,000 inhabitants), with 3445 cases. The number of cases declined sharply and decreased from 3445 cases in 2010 to 8 cases in 2014 following the control action plan interventions. The total of patients was diagnosed only on clinical basis and the lesions were considered caused by L. major. The epidemiological study was conducted between 2001 and 2014 and the molecular detection of CL was studied to identify the circulating parasite species in this province by using the ITS1-PCR-RFLP methods. In 2010, the molecular identification of 11 samples revealed the presence of L. major in the most affected circles: Goulmima, Er-Rissani, and Errachidia. In 2014 the molecular characterization of 7 among 8 cases reported in this year showed the presence of L. tropica in Errachidia circle. This is the first molecular identification of L. tropica in Errachidia province. The detection of this species after the intensified control measures strategies suggests that it was probably dissipated through the dominance of L. major.

No MeSH data available.


Related in: MedlinePlus