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Geographical Distribution and New Situation of Leishmania Species after the Control of Cutaneous Leishmaniasis Foci in Errachidia Province, Morocco, in 2014.

Mohamed Mahmoud el A, Faiza S, Lemine M, Smaine C, Adlaoui el B, Khalid H, Abderrahim S, Hajiba F - Biomed Res Int (2016)

Bottom Line: The number of cases declined sharply and decreased from 3445 cases in 2010 to 8 cases in 2014 following the control action plan interventions.This is the first molecular identification of L. tropica in Errachidia province.The detection of this species after the intensified control measures strategies suggests that it was probably dissipated through the dominance of L. major.

View Article: PubMed Central - PubMed

Affiliation: National Reference Laboratory of Leishmaniasis, National Institute of Hygiene, 27 Avenue Ibn Battuta, Agdal, 11400 Rabat, Morocco; Laboratory of Zoology and General Biology, Faculty of Sciences, Mohammed V University in Rabat, Avenue Ibn Battouta, BP 1014, 10000 Rabat, Morocco.

ABSTRACT
In Errachidia province, the incidence of cutaneous leishmaniasis (CL) has increased over the past decade and it was higher in 2010 (860.34 per 100,000 inhabitants), with 3445 cases. The number of cases declined sharply and decreased from 3445 cases in 2010 to 8 cases in 2014 following the control action plan interventions. The total of patients was diagnosed only on clinical basis and the lesions were considered caused by L. major. The epidemiological study was conducted between 2001 and 2014 and the molecular detection of CL was studied to identify the circulating parasite species in this province by using the ITS1-PCR-RFLP methods. In 2010, the molecular identification of 11 samples revealed the presence of L. major in the most affected circles: Goulmima, Er-Rissani, and Errachidia. In 2014 the molecular characterization of 7 among 8 cases reported in this year showed the presence of L. tropica in Errachidia circle. This is the first molecular identification of L. tropica in Errachidia province. The detection of this species after the intensified control measures strategies suggests that it was probably dissipated through the dominance of L. major.

No MeSH data available.


Related in: MedlinePlus

Gel electrophoresis of Leishmania (ITS1-PCR product) from Giemsa-stained lesion smears isolates obtained from patients. Lanes 1–10 correspond to clinical materials from patients. Sample from slides, lane 11: positive control, lane 12: negative control, and WM: weight marker, 100 pb.
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fig6: Gel electrophoresis of Leishmania (ITS1-PCR product) from Giemsa-stained lesion smears isolates obtained from patients. Lanes 1–10 correspond to clinical materials from patients. Sample from slides, lane 11: positive control, lane 12: negative control, and WM: weight marker, 100 pb.

Mentions: On the other hand, molecular identification revealed for the first time the presence of L. tropica (Figures 6 and 7). We think that the presence of this species could be related to semiarid climate in part of High Atlas in this region where the conditions are favorable, which is consistent with the weak distribution of its vector P. sergenti [10]. Therefore, we can say that L. tropica was present but at low density compared to L. major.


Geographical Distribution and New Situation of Leishmania Species after the Control of Cutaneous Leishmaniasis Foci in Errachidia Province, Morocco, in 2014.

Mohamed Mahmoud el A, Faiza S, Lemine M, Smaine C, Adlaoui el B, Khalid H, Abderrahim S, Hajiba F - Biomed Res Int (2016)

Gel electrophoresis of Leishmania (ITS1-PCR product) from Giemsa-stained lesion smears isolates obtained from patients. Lanes 1–10 correspond to clinical materials from patients. Sample from slides, lane 11: positive control, lane 12: negative control, and WM: weight marker, 100 pb.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940537&req=5

fig6: Gel electrophoresis of Leishmania (ITS1-PCR product) from Giemsa-stained lesion smears isolates obtained from patients. Lanes 1–10 correspond to clinical materials from patients. Sample from slides, lane 11: positive control, lane 12: negative control, and WM: weight marker, 100 pb.
Mentions: On the other hand, molecular identification revealed for the first time the presence of L. tropica (Figures 6 and 7). We think that the presence of this species could be related to semiarid climate in part of High Atlas in this region where the conditions are favorable, which is consistent with the weak distribution of its vector P. sergenti [10]. Therefore, we can say that L. tropica was present but at low density compared to L. major.

Bottom Line: The number of cases declined sharply and decreased from 3445 cases in 2010 to 8 cases in 2014 following the control action plan interventions.This is the first molecular identification of L. tropica in Errachidia province.The detection of this species after the intensified control measures strategies suggests that it was probably dissipated through the dominance of L. major.

View Article: PubMed Central - PubMed

Affiliation: National Reference Laboratory of Leishmaniasis, National Institute of Hygiene, 27 Avenue Ibn Battuta, Agdal, 11400 Rabat, Morocco; Laboratory of Zoology and General Biology, Faculty of Sciences, Mohammed V University in Rabat, Avenue Ibn Battouta, BP 1014, 10000 Rabat, Morocco.

ABSTRACT
In Errachidia province, the incidence of cutaneous leishmaniasis (CL) has increased over the past decade and it was higher in 2010 (860.34 per 100,000 inhabitants), with 3445 cases. The number of cases declined sharply and decreased from 3445 cases in 2010 to 8 cases in 2014 following the control action plan interventions. The total of patients was diagnosed only on clinical basis and the lesions were considered caused by L. major. The epidemiological study was conducted between 2001 and 2014 and the molecular detection of CL was studied to identify the circulating parasite species in this province by using the ITS1-PCR-RFLP methods. In 2010, the molecular identification of 11 samples revealed the presence of L. major in the most affected circles: Goulmima, Er-Rissani, and Errachidia. In 2014 the molecular characterization of 7 among 8 cases reported in this year showed the presence of L. tropica in Errachidia circle. This is the first molecular identification of L. tropica in Errachidia province. The detection of this species after the intensified control measures strategies suggests that it was probably dissipated through the dominance of L. major.

No MeSH data available.


Related in: MedlinePlus