Limits...
Genetic admixture and lineage separation in a southern Andean plant

View Article: PubMed Central - PubMed

ABSTRACT

Mountain orogeny has been a major factor in plant evolution in all continents by changing the landscape and climate, creating new habitats and ecological opportunities. In this study we found that diversity in two southern Andean Escallonia species is geographically structured and there is a deep divergence between infraspecific groups that could be associated with ancient evolutionary events like orogeny. We also found evidence of admixture, likely the result of hybridization at the margins of the parental species' distribution range.

No MeSH data available.


Geographical location of the 14 populations of Escallonia sampled, as listed in Table 1. Colours indicate groups according to taxonomy and morphological identifications: red: E. alpina var. carmelitana; green: E. rubra; blue: E. alpina var. alpina; fuchsia: IM.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4940511&req=5

plw034-F1: Geographical location of the 14 populations of Escallonia sampled, as listed in Table 1. Colours indicate groups according to taxonomy and morphological identifications: red: E. alpina var. carmelitana; green: E. rubra; blue: E. alpina var. alpina; fuchsia: IM.

Mentions: AFLP was used to analyze the genetic variability, both at the species and population level. A total of 14 populations (94 individuals) were kept based on the presence of good-quality DNA samples: four of E.alpina var.alpina, two of E. alpina var.carmelitana, six of E. rubra, and two populations with intermediate morphological diagnostic characters between both species (IM) (Table 1, Fig. 1). AFLP protocol was performed essentially as described by Vos et al. (1995), with fluorescent labelled primers that allowed automatic detection of the amplified fragments. Genomic DNA samples (50–100 ng) were digested to completion with EcoRI and MseI and the fragment ends were ligated to EcoRI- and MseI-specific adaptors [Supporting Information 2] in a single reaction for three hours at 37 °C. The digestion-ligation products were diluted 20-fold into 10 mM Tris-HCl, 0.1 mM EDTA (pH 8.0) and amplified using EcoRI-A and MseI-C as pre-selective primers. The resulting template was diluted 20-fold prior to amplification with selective primers: EcoRI (FAM)-ACT and MseI-CAC [Supporting Information 2]. The fluorescence-labelled selected amplification products were separated on a sequencer with an internal size standard at Macrogen Inc. (South Korea). Nine random samples (9.6 % of individuals) were duplicated in order to assess reproducibility.


Genetic admixture and lineage separation in a southern Andean plant
Geographical location of the 14 populations of Escallonia sampled, as listed in Table 1. Colours indicate groups according to taxonomy and morphological identifications: red: E. alpina var. carmelitana; green: E. rubra; blue: E. alpina var. alpina; fuchsia: IM.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940511&req=5

plw034-F1: Geographical location of the 14 populations of Escallonia sampled, as listed in Table 1. Colours indicate groups according to taxonomy and morphological identifications: red: E. alpina var. carmelitana; green: E. rubra; blue: E. alpina var. alpina; fuchsia: IM.
Mentions: AFLP was used to analyze the genetic variability, both at the species and population level. A total of 14 populations (94 individuals) were kept based on the presence of good-quality DNA samples: four of E.alpina var.alpina, two of E. alpina var.carmelitana, six of E. rubra, and two populations with intermediate morphological diagnostic characters between both species (IM) (Table 1, Fig. 1). AFLP protocol was performed essentially as described by Vos et al. (1995), with fluorescent labelled primers that allowed automatic detection of the amplified fragments. Genomic DNA samples (50–100 ng) were digested to completion with EcoRI and MseI and the fragment ends were ligated to EcoRI- and MseI-specific adaptors [Supporting Information 2] in a single reaction for three hours at 37 °C. The digestion-ligation products were diluted 20-fold into 10 mM Tris-HCl, 0.1 mM EDTA (pH 8.0) and amplified using EcoRI-A and MseI-C as pre-selective primers. The resulting template was diluted 20-fold prior to amplification with selective primers: EcoRI (FAM)-ACT and MseI-CAC [Supporting Information 2]. The fluorescence-labelled selected amplification products were separated on a sequencer with an internal size standard at Macrogen Inc. (South Korea). Nine random samples (9.6 % of individuals) were duplicated in order to assess reproducibility.

View Article: PubMed Central - PubMed

ABSTRACT

Mountain orogeny has been a major factor in plant evolution in all continents by changing the landscape and climate, creating new habitats and ecological opportunities. In this study we found that diversity in two southern Andean Escallonia species is geographically structured and there is a deep divergence between infraspecific groups that could be associated with ancient evolutionary events like orogeny. We also found evidence of admixture, likely the result of hybridization at the margins of the parental species' distribution range.

No MeSH data available.