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Adeno-Associated Virus 2-Mediated Hepatocellular Carcinoma is Very Rare in Korean Patients

View Article: PubMed Central - PubMed

ABSTRACT

Background: The incidence and etiology of hepatocellular carcinoma (HCC) vary widely according to race and geographic regions. The insertional mutagenesis of adeno-associated virus 2 (AAV2) has recently been considered a new viral etiology of HCC. The aim of this study was to investigate the frequency and clinical characteristics of AAV2 in Korean patients with HCC.

Methods: A total of 289 unrelated Korean patients with HCC, including 159 Hepatitis-B-related cases, 16 Hepatitis-C-related cases, and 114 viral serology-negative cases, who underwent surgery at the Samsung Medical Center in Korea from 2009 to 2014 were enrolled in this study. The presence of AAV2 in fresh-frozen tumor tissues was investigated by DNA PCR and Sanger sequencing. The clinical and pathological characteristics of AAV2-associated HCC in these patients were compared with previous findings in French patients.

Results: The AAV2 detection rate in Korean patients (2/289) was very low compared with that in French patients (11/193). Similar to the French patients, the Korean patients with AAV2-related HCC showed no signs of liver cirrhosis. The Korean patients were younger than the French patients with the same AAV2-associated HCC; the ages at diagnosis of the two Korean patients were 47 and 39 yr, while the median age of the 11 French patients was 55 yr (range 43-90 yr).

Conclusions: AAV2-associated HCC was very rare in Korean patients with HCC. Despite a limited number of cases, this study is the first to report the clinical characteristics of Korean patients with AAV2-associated HCC. These findings suggest epidemiologic differences in viral hepatocarcinogenesis between Korean and European patients.

No MeSH data available.


Related in: MedlinePlus

Genomic sequences of the adeno-associated virus 2 (AAV2) and the viral fragments that were identified in the Korean patients with hepatocellular carcinoma. The reference genome of AAV2 is AF043303.1.Abbreviations: ORF, open reading frame; ITR, inverse tandem repeat; Rep, replication protein; VP, viral capsid protein.
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Figure 1: Genomic sequences of the adeno-associated virus 2 (AAV2) and the viral fragments that were identified in the Korean patients with hepatocellular carcinoma. The reference genome of AAV2 is AF043303.1.Abbreviations: ORF, open reading frame; ITR, inverse tandem repeat; Rep, replication protein; VP, viral capsid protein.

Mentions: We found AAV2 fragments in the samples of only two of the 289 Korean patients (0.69%) by DNA PCR and Sanger sequencing. The viral fragments involved the 5' inverse tandem repeat (ITR), replication proteins (Rep), and capsid protein (VP1) (Fig. 1). The mRNA-seq results, which were available only in Case 1, showed no remarkable findings, such as fusion transcripts or RNA-processing events.


Adeno-Associated Virus 2-Mediated Hepatocellular Carcinoma is Very Rare in Korean Patients
Genomic sequences of the adeno-associated virus 2 (AAV2) and the viral fragments that were identified in the Korean patients with hepatocellular carcinoma. The reference genome of AAV2 is AF043303.1.Abbreviations: ORF, open reading frame; ITR, inverse tandem repeat; Rep, replication protein; VP, viral capsid protein.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940491&req=5

Figure 1: Genomic sequences of the adeno-associated virus 2 (AAV2) and the viral fragments that were identified in the Korean patients with hepatocellular carcinoma. The reference genome of AAV2 is AF043303.1.Abbreviations: ORF, open reading frame; ITR, inverse tandem repeat; Rep, replication protein; VP, viral capsid protein.
Mentions: We found AAV2 fragments in the samples of only two of the 289 Korean patients (0.69%) by DNA PCR and Sanger sequencing. The viral fragments involved the 5' inverse tandem repeat (ITR), replication proteins (Rep), and capsid protein (VP1) (Fig. 1). The mRNA-seq results, which were available only in Case 1, showed no remarkable findings, such as fusion transcripts or RNA-processing events.

View Article: PubMed Central - PubMed

ABSTRACT

Background: The incidence and etiology of hepatocellular carcinoma (HCC) vary widely according to race and geographic regions. The insertional mutagenesis of adeno-associated virus 2 (AAV2) has recently been considered a new viral etiology of HCC. The aim of this study was to investigate the frequency and clinical characteristics of AAV2 in Korean patients with HCC.

Methods: A total of 289 unrelated Korean patients with HCC, including 159 Hepatitis-B-related cases, 16 Hepatitis-C-related cases, and 114 viral serology-negative cases, who underwent surgery at the Samsung Medical Center in Korea from 2009 to 2014 were enrolled in this study. The presence of AAV2 in fresh-frozen tumor tissues was investigated by DNA PCR and Sanger sequencing. The clinical and pathological characteristics of AAV2-associated HCC in these patients were compared with previous findings in French patients.

Results: The AAV2 detection rate in Korean patients (2/289) was very low compared with that in French patients (11/193). Similar to the French patients, the Korean patients with AAV2-related HCC showed no signs of liver cirrhosis. The Korean patients were younger than the French patients with the same AAV2-associated HCC; the ages at diagnosis of the two Korean patients were 47 and 39 yr, while the median age of the 11 French patients was 55 yr (range 43-90 yr).

Conclusions: AAV2-associated HCC was very rare in Korean patients with HCC. Despite a limited number of cases, this study is the first to report the clinical characteristics of Korean patients with AAV2-associated HCC. These findings suggest epidemiologic differences in viral hepatocarcinogenesis between Korean and European patients.

No MeSH data available.


Related in: MedlinePlus