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Comparative miRNA-Based Fingerprinting Reveals Biological Differences in Human Olfactory Mucosa- and Bone-Marrow-Derived Mesenchymal Stromal Cells.

Lindsay SL, Johnstone SA, McGrath MA, Mallinson D, Barnett SC - Stem Cell Reports (2016)

Bottom Line: Previously we reported that nestin-positive human mesenchymal stromal cells (MSCs) derived from the olfactory mucosa (OM) enhanced CNS myelination in vitro to a greater extent than bone-marrow-derived MSCs (BM-MSCs). miRNA-based fingerprinting revealed the two MSCs were 64% homologous, with 26 miRNAs differentially expressed.The lower expression of miR-140-5p in OM-MSCs correlated with higher secretion of CXCL12 compared with BM-MSCs.Nestin-positive OM-MSCs could therefore offer a cell transplantation alternative for CNS repair, should these biological behaviors be translated in vivo.

View Article: PubMed Central - PubMed

Affiliation: Institute of Infection, Inflammation and Immunity, Glasgow Biomedical Research Centre, University of Glasgow, Sir Graeme Davies Building, 120 University Place, Glasgow G12 8TA, UK.

No MeSH data available.


Related in: MedlinePlus

MiR-146-5p Differentially Regulates TLR4, TLR2, and Cytokine Secretion(A) Graphical representation of TLR4 and TLR2 (western blot shown as inserts) in BM-MSCs (n = 6 patient samples) and OM-MSCs (n = 6 patient samples). BM-MSCs have significantly higher levels compared with OM-MSCs (mean ± SEM, ∗p < 0.05, ∗∗p < 0.01, Students unpaired t test).(B) FACS analysis of TLR4 expression on OM- and BM-MSCs (n = 3, patient samples both).(C) OM-MSC (n = 1 patient sample) and BM-MSC (n = 1 patient sample) cytokine profile. Insert shows dot plot while the graph illustrates the mean pixel intensity of the blot. IL-6 (1) IL-8 (2), and CCL2 (3) were expressed 1.5-fold higher in BM-MSC-CM.(D) ELISA analysis of IL-8, IL-6, and CCL2 in BM-MSC-CM (n = 3 patient samples) and OM-MSC-CM (n = 3 patient samples, mean ± SEM, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, Students unpaired t test).
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fig5: MiR-146-5p Differentially Regulates TLR4, TLR2, and Cytokine Secretion(A) Graphical representation of TLR4 and TLR2 (western blot shown as inserts) in BM-MSCs (n = 6 patient samples) and OM-MSCs (n = 6 patient samples). BM-MSCs have significantly higher levels compared with OM-MSCs (mean ± SEM, ∗p < 0.05, ∗∗p < 0.01, Students unpaired t test).(B) FACS analysis of TLR4 expression on OM- and BM-MSCs (n = 3, patient samples both).(C) OM-MSC (n = 1 patient sample) and BM-MSC (n = 1 patient sample) cytokine profile. Insert shows dot plot while the graph illustrates the mean pixel intensity of the blot. IL-6 (1) IL-8 (2), and CCL2 (3) were expressed 1.5-fold higher in BM-MSC-CM.(D) ELISA analysis of IL-8, IL-6, and CCL2 in BM-MSC-CM (n = 3 patient samples) and OM-MSC-CM (n = 3 patient samples, mean ± SEM, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, Students unpaired t test).

Mentions: The GeneGo MetaCore network for miR-146a-5p indicated an association with Toll-like receptor (TLR) expression and the regulation of inflammatory chemokines. This suggests differential modulation of the inflammatory response, which is an important consideration for cell transplant-mediated repair. Total TLR2 and 4 levels were significantly less in OM-MSCs compared with BM-MSCs (n = 6 patient samples for both, p < 0.01 and p < 0.05, respectively; Figure 5A). There was notably less TLR2 expression in both cell types compared with their TLR4 expression since only TLR4 was present abundantly enough to be detected via fluorescence-activated cell sorting (FACS) (n = 3 patient samples for both; Figure 5B). MiR-146a-5p is thought to modulate the secretion of IL-6 and IL-8, and BM-MSC-CM was found to contain at least 1.5-fold more of both these and CCL2 than OM-MSC-CM (Figure 5C). Quantification was carried out by ELISA (n = 3 patient samples for both, p < 0.01, p < 0.001, and p < 0.05; Figure 5D).


Comparative miRNA-Based Fingerprinting Reveals Biological Differences in Human Olfactory Mucosa- and Bone-Marrow-Derived Mesenchymal Stromal Cells.

Lindsay SL, Johnstone SA, McGrath MA, Mallinson D, Barnett SC - Stem Cell Reports (2016)

MiR-146-5p Differentially Regulates TLR4, TLR2, and Cytokine Secretion(A) Graphical representation of TLR4 and TLR2 (western blot shown as inserts) in BM-MSCs (n = 6 patient samples) and OM-MSCs (n = 6 patient samples). BM-MSCs have significantly higher levels compared with OM-MSCs (mean ± SEM, ∗p < 0.05, ∗∗p < 0.01, Students unpaired t test).(B) FACS analysis of TLR4 expression on OM- and BM-MSCs (n = 3, patient samples both).(C) OM-MSC (n = 1 patient sample) and BM-MSC (n = 1 patient sample) cytokine profile. Insert shows dot plot while the graph illustrates the mean pixel intensity of the blot. IL-6 (1) IL-8 (2), and CCL2 (3) were expressed 1.5-fold higher in BM-MSC-CM.(D) ELISA analysis of IL-8, IL-6, and CCL2 in BM-MSC-CM (n = 3 patient samples) and OM-MSC-CM (n = 3 patient samples, mean ± SEM, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, Students unpaired t test).
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fig5: MiR-146-5p Differentially Regulates TLR4, TLR2, and Cytokine Secretion(A) Graphical representation of TLR4 and TLR2 (western blot shown as inserts) in BM-MSCs (n = 6 patient samples) and OM-MSCs (n = 6 patient samples). BM-MSCs have significantly higher levels compared with OM-MSCs (mean ± SEM, ∗p < 0.05, ∗∗p < 0.01, Students unpaired t test).(B) FACS analysis of TLR4 expression on OM- and BM-MSCs (n = 3, patient samples both).(C) OM-MSC (n = 1 patient sample) and BM-MSC (n = 1 patient sample) cytokine profile. Insert shows dot plot while the graph illustrates the mean pixel intensity of the blot. IL-6 (1) IL-8 (2), and CCL2 (3) were expressed 1.5-fold higher in BM-MSC-CM.(D) ELISA analysis of IL-8, IL-6, and CCL2 in BM-MSC-CM (n = 3 patient samples) and OM-MSC-CM (n = 3 patient samples, mean ± SEM, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, Students unpaired t test).
Mentions: The GeneGo MetaCore network for miR-146a-5p indicated an association with Toll-like receptor (TLR) expression and the regulation of inflammatory chemokines. This suggests differential modulation of the inflammatory response, which is an important consideration for cell transplant-mediated repair. Total TLR2 and 4 levels were significantly less in OM-MSCs compared with BM-MSCs (n = 6 patient samples for both, p < 0.01 and p < 0.05, respectively; Figure 5A). There was notably less TLR2 expression in both cell types compared with their TLR4 expression since only TLR4 was present abundantly enough to be detected via fluorescence-activated cell sorting (FACS) (n = 3 patient samples for both; Figure 5B). MiR-146a-5p is thought to modulate the secretion of IL-6 and IL-8, and BM-MSC-CM was found to contain at least 1.5-fold more of both these and CCL2 than OM-MSC-CM (Figure 5C). Quantification was carried out by ELISA (n = 3 patient samples for both, p < 0.01, p < 0.001, and p < 0.05; Figure 5D).

Bottom Line: Previously we reported that nestin-positive human mesenchymal stromal cells (MSCs) derived from the olfactory mucosa (OM) enhanced CNS myelination in vitro to a greater extent than bone-marrow-derived MSCs (BM-MSCs). miRNA-based fingerprinting revealed the two MSCs were 64% homologous, with 26 miRNAs differentially expressed.The lower expression of miR-140-5p in OM-MSCs correlated with higher secretion of CXCL12 compared with BM-MSCs.Nestin-positive OM-MSCs could therefore offer a cell transplantation alternative for CNS repair, should these biological behaviors be translated in vivo.

View Article: PubMed Central - PubMed

Affiliation: Institute of Infection, Inflammation and Immunity, Glasgow Biomedical Research Centre, University of Glasgow, Sir Graeme Davies Building, 120 University Place, Glasgow G12 8TA, UK.

No MeSH data available.


Related in: MedlinePlus