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Comparative miRNA-Based Fingerprinting Reveals Biological Differences in Human Olfactory Mucosa- and Bone-Marrow-Derived Mesenchymal Stromal Cells.

Lindsay SL, Johnstone SA, McGrath MA, Mallinson D, Barnett SC - Stem Cell Reports (2016)

Bottom Line: Previously we reported that nestin-positive human mesenchymal stromal cells (MSCs) derived from the olfactory mucosa (OM) enhanced CNS myelination in vitro to a greater extent than bone-marrow-derived MSCs (BM-MSCs). miRNA-based fingerprinting revealed the two MSCs were 64% homologous, with 26 miRNAs differentially expressed.The lower expression of miR-140-5p in OM-MSCs correlated with higher secretion of CXCL12 compared with BM-MSCs.Nestin-positive OM-MSCs could therefore offer a cell transplantation alternative for CNS repair, should these biological behaviors be translated in vivo.

View Article: PubMed Central - PubMed

Affiliation: Institute of Infection, Inflammation and Immunity, Glasgow Biomedical Research Centre, University of Glasgow, Sir Graeme Davies Building, 120 University Place, Glasgow G12 8TA, UK.

No MeSH data available.


Related in: MedlinePlus

miRNA Profiling of OM-MSCs and BM-MSCs(A) Twenty-seven equivocally expressed (EE) miRNAs in OM-MSCs (n = 4 patient samples) and BM-MSCs (n = 4 patient samples) that associate specifically with MSCs (significance determined at PFDR < 0.05 rejects the  hypothesis and concludes that they are EE).(B) Twenty-six differentially expressed (DE) miRNAs between both cell types. Table shows the fold change (FC) in expression of BM-MSCs versus OM-MSCs (n = 4 patient samples, significance at PFDR < 0.05 and an FC ≥ 1.5). Two miRNAs of interest, hsa-miR-140-5p and hsa-miR-146a-5p, are highlighted in red.(C) qPCR confirms miR-140-5p is significantly upregulated in BM-MSCs compared with OM-MSCs (n = 4 patient samples, mean ± SEM, ∗p < 0.05 determined by one-way ANOVA, Tukey's multiple comparison).(D) qPCR confirms miR-146-5p is upregulated in OM-MSCs compared with BM-MSCs (n = 4 patient samples, mean ± SEM, ∗p < 0.05, determined by one-way ANOVA, Tukey's multiple comparison).(E) FC in expression of miR-140-5p and miR-146-5p in OM-MSCs versus BM-MSCs.
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fig1: miRNA Profiling of OM-MSCs and BM-MSCs(A) Twenty-seven equivocally expressed (EE) miRNAs in OM-MSCs (n = 4 patient samples) and BM-MSCs (n = 4 patient samples) that associate specifically with MSCs (significance determined at PFDR < 0.05 rejects the hypothesis and concludes that they are EE).(B) Twenty-six differentially expressed (DE) miRNAs between both cell types. Table shows the fold change (FC) in expression of BM-MSCs versus OM-MSCs (n = 4 patient samples, significance at PFDR < 0.05 and an FC ≥ 1.5). Two miRNAs of interest, hsa-miR-140-5p and hsa-miR-146a-5p, are highlighted in red.(C) qPCR confirms miR-140-5p is significantly upregulated in BM-MSCs compared with OM-MSCs (n = 4 patient samples, mean ± SEM, ∗p < 0.05 determined by one-way ANOVA, Tukey's multiple comparison).(D) qPCR confirms miR-146-5p is upregulated in OM-MSCs compared with BM-MSCs (n = 4 patient samples, mean ± SEM, ∗p < 0.05, determined by one-way ANOVA, Tukey's multiple comparison).(E) FC in expression of miR-140-5p and miR-146-5p in OM-MSCs versus BM-MSCs.

Mentions: Analysis revealed 195 mature miRNAs detected in OM-MSCs (n = 4 patient samples) and BM-MSCs (n = 4 patient samples), of which 125 were equivalently expressed (EE). This demonstrates 64% identity, despite being isolated from different tissues; moreover, 27 of these EE miRNAs have already been reported for BM-MSCs (Gao et al., 2011; Figure 1A). These data suggest that MSCs derived from OM express a similar core set of miRNAs compared with BM.


Comparative miRNA-Based Fingerprinting Reveals Biological Differences in Human Olfactory Mucosa- and Bone-Marrow-Derived Mesenchymal Stromal Cells.

Lindsay SL, Johnstone SA, McGrath MA, Mallinson D, Barnett SC - Stem Cell Reports (2016)

miRNA Profiling of OM-MSCs and BM-MSCs(A) Twenty-seven equivocally expressed (EE) miRNAs in OM-MSCs (n = 4 patient samples) and BM-MSCs (n = 4 patient samples) that associate specifically with MSCs (significance determined at PFDR < 0.05 rejects the  hypothesis and concludes that they are EE).(B) Twenty-six differentially expressed (DE) miRNAs between both cell types. Table shows the fold change (FC) in expression of BM-MSCs versus OM-MSCs (n = 4 patient samples, significance at PFDR < 0.05 and an FC ≥ 1.5). Two miRNAs of interest, hsa-miR-140-5p and hsa-miR-146a-5p, are highlighted in red.(C) qPCR confirms miR-140-5p is significantly upregulated in BM-MSCs compared with OM-MSCs (n = 4 patient samples, mean ± SEM, ∗p < 0.05 determined by one-way ANOVA, Tukey's multiple comparison).(D) qPCR confirms miR-146-5p is upregulated in OM-MSCs compared with BM-MSCs (n = 4 patient samples, mean ± SEM, ∗p < 0.05, determined by one-way ANOVA, Tukey's multiple comparison).(E) FC in expression of miR-140-5p and miR-146-5p in OM-MSCs versus BM-MSCs.
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fig1: miRNA Profiling of OM-MSCs and BM-MSCs(A) Twenty-seven equivocally expressed (EE) miRNAs in OM-MSCs (n = 4 patient samples) and BM-MSCs (n = 4 patient samples) that associate specifically with MSCs (significance determined at PFDR < 0.05 rejects the hypothesis and concludes that they are EE).(B) Twenty-six differentially expressed (DE) miRNAs between both cell types. Table shows the fold change (FC) in expression of BM-MSCs versus OM-MSCs (n = 4 patient samples, significance at PFDR < 0.05 and an FC ≥ 1.5). Two miRNAs of interest, hsa-miR-140-5p and hsa-miR-146a-5p, are highlighted in red.(C) qPCR confirms miR-140-5p is significantly upregulated in BM-MSCs compared with OM-MSCs (n = 4 patient samples, mean ± SEM, ∗p < 0.05 determined by one-way ANOVA, Tukey's multiple comparison).(D) qPCR confirms miR-146-5p is upregulated in OM-MSCs compared with BM-MSCs (n = 4 patient samples, mean ± SEM, ∗p < 0.05, determined by one-way ANOVA, Tukey's multiple comparison).(E) FC in expression of miR-140-5p and miR-146-5p in OM-MSCs versus BM-MSCs.
Mentions: Analysis revealed 195 mature miRNAs detected in OM-MSCs (n = 4 patient samples) and BM-MSCs (n = 4 patient samples), of which 125 were equivalently expressed (EE). This demonstrates 64% identity, despite being isolated from different tissues; moreover, 27 of these EE miRNAs have already been reported for BM-MSCs (Gao et al., 2011; Figure 1A). These data suggest that MSCs derived from OM express a similar core set of miRNAs compared with BM.

Bottom Line: Previously we reported that nestin-positive human mesenchymal stromal cells (MSCs) derived from the olfactory mucosa (OM) enhanced CNS myelination in vitro to a greater extent than bone-marrow-derived MSCs (BM-MSCs). miRNA-based fingerprinting revealed the two MSCs were 64% homologous, with 26 miRNAs differentially expressed.The lower expression of miR-140-5p in OM-MSCs correlated with higher secretion of CXCL12 compared with BM-MSCs.Nestin-positive OM-MSCs could therefore offer a cell transplantation alternative for CNS repair, should these biological behaviors be translated in vivo.

View Article: PubMed Central - PubMed

Affiliation: Institute of Infection, Inflammation and Immunity, Glasgow Biomedical Research Centre, University of Glasgow, Sir Graeme Davies Building, 120 University Place, Glasgow G12 8TA, UK.

No MeSH data available.


Related in: MedlinePlus