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Near Infrared Imaging As a Method of Studying Tsetse Fly (Diptera: Glossinidae) Pupal Development.

Moran ZR, Parker AG - J. Insect Sci. (2016)

Bottom Line: Various wavelengths of NIR light from 880 to 1060 nm were compared to study the development of tsetse fly pupae from larviposition to emergence, using time-lapse videos and photographs.In addition, it presents a new methodology for studying the pupal stage of many coarctate insects for many applications.NIR imaging permits observation of living pupae, allowing the entire development process to be observed without disruption.

View Article: PubMed Central - PubMed

Affiliation: Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, P.O. Box 100, 1400 Vienna, Austria (zelda.moran@gmail.com; a.g.parker@iaea.org).

No MeSH data available.


Related in: MedlinePlus

Anterior view of Glossina palpalis gambiensis puparia under 1060 nm NIR light: (A) Day zero, no structures visible; (B) Day 6, eyes visible, frons visible, no pigmentation; (C) Day 20, eyes begin to darken; (D) Day 25, eyes darkened; and (E) Day 33, eyes and frons darkened, air bubbles begin pushing tissue away from the inside of the puparium before emergence.
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iew047-F8: Anterior view of Glossina palpalis gambiensis puparia under 1060 nm NIR light: (A) Day zero, no structures visible; (B) Day 6, eyes visible, frons visible, no pigmentation; (C) Day 20, eyes begin to darken; (D) Day 25, eyes darkened; and (E) Day 33, eyes and frons darkened, air bubbles begin pushing tissue away from the inside of the puparium before emergence.

Mentions: Similar to ventral position, the first outlines of structures are visible on day 6, when the eyes, frons, and a part of the abdomen become visible. Again, no further changes occur until pigmentation begins around days 24–27. Eyes, frons, and wings darken, and bristles become visible on the abdomen. In the days before emergence, all structures darken in color, but eyes and frons may shrink in size and sometimes disappear altogether, presumably due to air pockets developing and separating the pharate adult from the inside of the pupal shell in preparation for emergence. This would also be in keeping with the findings of Bursell (1958, 1959) that moisture is absorbed during the days leading up to emergence. An unidentified tree-shaped pattern of markings was often visible on the dorsum of the pupa (Fig. 7).


Near Infrared Imaging As a Method of Studying Tsetse Fly (Diptera: Glossinidae) Pupal Development.

Moran ZR, Parker AG - J. Insect Sci. (2016)

Anterior view of Glossina palpalis gambiensis puparia under 1060 nm NIR light: (A) Day zero, no structures visible; (B) Day 6, eyes visible, frons visible, no pigmentation; (C) Day 20, eyes begin to darken; (D) Day 25, eyes darkened; and (E) Day 33, eyes and frons darkened, air bubbles begin pushing tissue away from the inside of the puparium before emergence.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940448&req=5

iew047-F8: Anterior view of Glossina palpalis gambiensis puparia under 1060 nm NIR light: (A) Day zero, no structures visible; (B) Day 6, eyes visible, frons visible, no pigmentation; (C) Day 20, eyes begin to darken; (D) Day 25, eyes darkened; and (E) Day 33, eyes and frons darkened, air bubbles begin pushing tissue away from the inside of the puparium before emergence.
Mentions: Similar to ventral position, the first outlines of structures are visible on day 6, when the eyes, frons, and a part of the abdomen become visible. Again, no further changes occur until pigmentation begins around days 24–27. Eyes, frons, and wings darken, and bristles become visible on the abdomen. In the days before emergence, all structures darken in color, but eyes and frons may shrink in size and sometimes disappear altogether, presumably due to air pockets developing and separating the pharate adult from the inside of the pupal shell in preparation for emergence. This would also be in keeping with the findings of Bursell (1958, 1959) that moisture is absorbed during the days leading up to emergence. An unidentified tree-shaped pattern of markings was often visible on the dorsum of the pupa (Fig. 7).

Bottom Line: Various wavelengths of NIR light from 880 to 1060 nm were compared to study the development of tsetse fly pupae from larviposition to emergence, using time-lapse videos and photographs.In addition, it presents a new methodology for studying the pupal stage of many coarctate insects for many applications.NIR imaging permits observation of living pupae, allowing the entire development process to be observed without disruption.

View Article: PubMed Central - PubMed

Affiliation: Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, P.O. Box 100, 1400 Vienna, Austria (zelda.moran@gmail.com; a.g.parker@iaea.org).

No MeSH data available.


Related in: MedlinePlus