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Near Infrared Imaging As a Method of Studying Tsetse Fly (Diptera: Glossinidae) Pupal Development.

Moran ZR, Parker AG - J. Insect Sci. (2016)

Bottom Line: Various wavelengths of NIR light from 880 to 1060 nm were compared to study the development of tsetse fly pupae from larviposition to emergence, using time-lapse videos and photographs.In addition, it presents a new methodology for studying the pupal stage of many coarctate insects for many applications.NIR imaging permits observation of living pupae, allowing the entire development process to be observed without disruption.

View Article: PubMed Central - PubMed

Affiliation: Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, P.O. Box 100, 1400 Vienna, Austria (zelda.moran@gmail.com; a.g.parker@iaea.org).

No MeSH data available.


Related in: MedlinePlus

Dorsal view of Glossina palpalis gambiensis puparia under 1060 nm NIR light: (A) Day zero, no structures visible; (B) Day 6, eyes visible, frons visible; (C) Day 11, No further developments; (D) Day 19, No further developments; and (E) Day 28, pigmentation in wings, eyes, frons, and bristles is visible. We also observe an unidentified branching pattern on the back; (F) Day 33, bristles are darkened and more visible near the posterior end. Eyes appear more defined and smaller because of air pockets between them and the puparium, which seem to develop just before emergence.
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iew047-F7: Dorsal view of Glossina palpalis gambiensis puparia under 1060 nm NIR light: (A) Day zero, no structures visible; (B) Day 6, eyes visible, frons visible; (C) Day 11, No further developments; (D) Day 19, No further developments; and (E) Day 28, pigmentation in wings, eyes, frons, and bristles is visible. We also observe an unidentified branching pattern on the back; (F) Day 33, bristles are darkened and more visible near the posterior end. Eyes appear more defined and smaller because of air pockets between them and the puparium, which seem to develop just before emergence.

Mentions: If observed during the first 24 h, the third instar puparium does not reveal any structures or appendages, but slight heartbeat-like movement is visible under video or if observed live under the microscope. On day 6, the outline of eyes, legs, and frons appear, in perfect keeping with the findings of Bursell (1958) that the pupa retracts from the inside of the puparial shell on this day. No further changes are observed until pigmentation begins around day 24 for females and 27 for males. Pigmentation begins in the wings and spreads through the legs and eyes. By day 28, eyes, wings, and legs are pigmented and will continue to darken until pre-emergence (day 32 onward).


Near Infrared Imaging As a Method of Studying Tsetse Fly (Diptera: Glossinidae) Pupal Development.

Moran ZR, Parker AG - J. Insect Sci. (2016)

Dorsal view of Glossina palpalis gambiensis puparia under 1060 nm NIR light: (A) Day zero, no structures visible; (B) Day 6, eyes visible, frons visible; (C) Day 11, No further developments; (D) Day 19, No further developments; and (E) Day 28, pigmentation in wings, eyes, frons, and bristles is visible. We also observe an unidentified branching pattern on the back; (F) Day 33, bristles are darkened and more visible near the posterior end. Eyes appear more defined and smaller because of air pockets between them and the puparium, which seem to develop just before emergence.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC4940448&req=5

iew047-F7: Dorsal view of Glossina palpalis gambiensis puparia under 1060 nm NIR light: (A) Day zero, no structures visible; (B) Day 6, eyes visible, frons visible; (C) Day 11, No further developments; (D) Day 19, No further developments; and (E) Day 28, pigmentation in wings, eyes, frons, and bristles is visible. We also observe an unidentified branching pattern on the back; (F) Day 33, bristles are darkened and more visible near the posterior end. Eyes appear more defined and smaller because of air pockets between them and the puparium, which seem to develop just before emergence.
Mentions: If observed during the first 24 h, the third instar puparium does not reveal any structures or appendages, but slight heartbeat-like movement is visible under video or if observed live under the microscope. On day 6, the outline of eyes, legs, and frons appear, in perfect keeping with the findings of Bursell (1958) that the pupa retracts from the inside of the puparial shell on this day. No further changes are observed until pigmentation begins around day 24 for females and 27 for males. Pigmentation begins in the wings and spreads through the legs and eyes. By day 28, eyes, wings, and legs are pigmented and will continue to darken until pre-emergence (day 32 onward).

Bottom Line: Various wavelengths of NIR light from 880 to 1060 nm were compared to study the development of tsetse fly pupae from larviposition to emergence, using time-lapse videos and photographs.In addition, it presents a new methodology for studying the pupal stage of many coarctate insects for many applications.NIR imaging permits observation of living pupae, allowing the entire development process to be observed without disruption.

View Article: PubMed Central - PubMed

Affiliation: Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, P.O. Box 100, 1400 Vienna, Austria (zelda.moran@gmail.com; a.g.parker@iaea.org).

No MeSH data available.


Related in: MedlinePlus