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Near Infrared Imaging As a Method of Studying Tsetse Fly (Diptera: Glossinidae) Pupal Development.

Moran ZR, Parker AG - J. Insect Sci. (2016)

Bottom Line: Various wavelengths of NIR light from 880 to 1060 nm were compared to study the development of tsetse fly pupae from larviposition to emergence, using time-lapse videos and photographs.In addition, it presents a new methodology for studying the pupal stage of many coarctate insects for many applications.NIR imaging permits observation of living pupae, allowing the entire development process to be observed without disruption.

View Article: PubMed Central - PubMed

Affiliation: Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, P.O. Box 100, 1400 Vienna, Austria (zelda.moran@gmail.com; a.g.parker@iaea.org).

No MeSH data available.


Related in: MedlinePlus

Ventral view of developing Glossina palpalis gambiensis puparia under 1060 nm NIR light: (A) Day zero, no structures visible; (B) Day 6, legs visible; (C) Day 12, no further developments; (D) Day 20 appendages slightly sharper; (E) Day 27, pigmentation has occurred in legs, wings, and eyes; and (F) Day 32, legs, wings, antennae, frons and eyes fully pigmented.
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iew047-F6: Ventral view of developing Glossina palpalis gambiensis puparia under 1060 nm NIR light: (A) Day zero, no structures visible; (B) Day 6, legs visible; (C) Day 12, no further developments; (D) Day 20 appendages slightly sharper; (E) Day 27, pigmentation has occurred in legs, wings, and eyes; and (F) Day 32, legs, wings, antennae, frons and eyes fully pigmented.

Mentions: It was quickly observed that the ventral view, under 1060 nm, revealed the most structures, including a notable difference in timing of wing pigmentation between males and females.


Near Infrared Imaging As a Method of Studying Tsetse Fly (Diptera: Glossinidae) Pupal Development.

Moran ZR, Parker AG - J. Insect Sci. (2016)

Ventral view of developing Glossina palpalis gambiensis puparia under 1060 nm NIR light: (A) Day zero, no structures visible; (B) Day 6, legs visible; (C) Day 12, no further developments; (D) Day 20 appendages slightly sharper; (E) Day 27, pigmentation has occurred in legs, wings, and eyes; and (F) Day 32, legs, wings, antennae, frons and eyes fully pigmented.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940448&req=5

iew047-F6: Ventral view of developing Glossina palpalis gambiensis puparia under 1060 nm NIR light: (A) Day zero, no structures visible; (B) Day 6, legs visible; (C) Day 12, no further developments; (D) Day 20 appendages slightly sharper; (E) Day 27, pigmentation has occurred in legs, wings, and eyes; and (F) Day 32, legs, wings, antennae, frons and eyes fully pigmented.
Mentions: It was quickly observed that the ventral view, under 1060 nm, revealed the most structures, including a notable difference in timing of wing pigmentation between males and females.

Bottom Line: Various wavelengths of NIR light from 880 to 1060 nm were compared to study the development of tsetse fly pupae from larviposition to emergence, using time-lapse videos and photographs.In addition, it presents a new methodology for studying the pupal stage of many coarctate insects for many applications.NIR imaging permits observation of living pupae, allowing the entire development process to be observed without disruption.

View Article: PubMed Central - PubMed

Affiliation: Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, P.O. Box 100, 1400 Vienna, Austria (zelda.moran@gmail.com; a.g.parker@iaea.org).

No MeSH data available.


Related in: MedlinePlus