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Near Infrared Imaging As a Method of Studying Tsetse Fly (Diptera: Glossinidae) Pupal Development.

Moran ZR, Parker AG - J. Insect Sci. (2016)

Bottom Line: Various wavelengths of NIR light from 880 to 1060 nm were compared to study the development of tsetse fly pupae from larviposition to emergence, using time-lapse videos and photographs.In addition, it presents a new methodology for studying the pupal stage of many coarctate insects for many applications.NIR imaging permits observation of living pupae, allowing the entire development process to be observed without disruption.

View Article: PubMed Central - PubMed

Affiliation: Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, P.O. Box 100, 1400 Vienna, Austria (zelda.moran@gmail.com; a.g.parker@iaea.org).

No MeSH data available.


Related in: MedlinePlus

Images of fully developed, 30-d-old pupae under 1060 nm NIR light and corresponding photos of the dissected pharate adult. In image (A), the eyes are visible and are fully pigmented at this stage. The ptilinum and antennae are also very prominent. Image (B) is a ventral view, and shows wings on the sides of the pupae, the proboscis in the center, and the legs spreading to each side. The ptilinum is also visible, as is a small section of the eyes. Images (C), (D), and (F) show the same pupae with the top of the pupa removed so that the structures are visible. Image (C) and (F) show the legs and proboscis visible in image (B) under NIR, and image (D) shows the eyes, ptilinum, and antennae visible under NIR in image (A). Image (E) shows the dorsal view, with the eyes, ptilinum, and hairs visible on the body. an, antennae; pt, ptilinum; pr, proboscis; ha, hair.
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iew047-F5: Images of fully developed, 30-d-old pupae under 1060 nm NIR light and corresponding photos of the dissected pharate adult. In image (A), the eyes are visible and are fully pigmented at this stage. The ptilinum and antennae are also very prominent. Image (B) is a ventral view, and shows wings on the sides of the pupae, the proboscis in the center, and the legs spreading to each side. The ptilinum is also visible, as is a small section of the eyes. Images (C), (D), and (F) show the same pupae with the top of the pupa removed so that the structures are visible. Image (C) and (F) show the legs and proboscis visible in image (B) under NIR, and image (D) shows the eyes, ptilinum, and antennae visible under NIR in image (A). Image (E) shows the dorsal view, with the eyes, ptilinum, and hairs visible on the body. an, antennae; pt, ptilinum; pr, proboscis; ha, hair.

Mentions: Images of fully developed pupae are shown under 1060 nm NIR illumination in Fig. 5.Fig. 5.


Near Infrared Imaging As a Method of Studying Tsetse Fly (Diptera: Glossinidae) Pupal Development.

Moran ZR, Parker AG - J. Insect Sci. (2016)

Images of fully developed, 30-d-old pupae under 1060 nm NIR light and corresponding photos of the dissected pharate adult. In image (A), the eyes are visible and are fully pigmented at this stage. The ptilinum and antennae are also very prominent. Image (B) is a ventral view, and shows wings on the sides of the pupae, the proboscis in the center, and the legs spreading to each side. The ptilinum is also visible, as is a small section of the eyes. Images (C), (D), and (F) show the same pupae with the top of the pupa removed so that the structures are visible. Image (C) and (F) show the legs and proboscis visible in image (B) under NIR, and image (D) shows the eyes, ptilinum, and antennae visible under NIR in image (A). Image (E) shows the dorsal view, with the eyes, ptilinum, and hairs visible on the body. an, antennae; pt, ptilinum; pr, proboscis; ha, hair.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940448&req=5

iew047-F5: Images of fully developed, 30-d-old pupae under 1060 nm NIR light and corresponding photos of the dissected pharate adult. In image (A), the eyes are visible and are fully pigmented at this stage. The ptilinum and antennae are also very prominent. Image (B) is a ventral view, and shows wings on the sides of the pupae, the proboscis in the center, and the legs spreading to each side. The ptilinum is also visible, as is a small section of the eyes. Images (C), (D), and (F) show the same pupae with the top of the pupa removed so that the structures are visible. Image (C) and (F) show the legs and proboscis visible in image (B) under NIR, and image (D) shows the eyes, ptilinum, and antennae visible under NIR in image (A). Image (E) shows the dorsal view, with the eyes, ptilinum, and hairs visible on the body. an, antennae; pt, ptilinum; pr, proboscis; ha, hair.
Mentions: Images of fully developed pupae are shown under 1060 nm NIR illumination in Fig. 5.Fig. 5.

Bottom Line: Various wavelengths of NIR light from 880 to 1060 nm were compared to study the development of tsetse fly pupae from larviposition to emergence, using time-lapse videos and photographs.In addition, it presents a new methodology for studying the pupal stage of many coarctate insects for many applications.NIR imaging permits observation of living pupae, allowing the entire development process to be observed without disruption.

View Article: PubMed Central - PubMed

Affiliation: Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, P.O. Box 100, 1400 Vienna, Austria (zelda.moran@gmail.com; a.g.parker@iaea.org).

No MeSH data available.


Related in: MedlinePlus