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Near Infrared Imaging As a Method of Studying Tsetse Fly (Diptera: Glossinidae) Pupal Development.

Moran ZR, Parker AG - J. Insect Sci. (2016)

Bottom Line: Various wavelengths of NIR light from 880 to 1060 nm were compared to study the development of tsetse fly pupae from larviposition to emergence, using time-lapse videos and photographs.In addition, it presents a new methodology for studying the pupal stage of many coarctate insects for many applications.NIR imaging permits observation of living pupae, allowing the entire development process to be observed without disruption.

View Article: PubMed Central - PubMed

Affiliation: Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, P.O. Box 100, 1400 Vienna, Austria (zelda.moran@gmail.com; a.g.parker@iaea.org).

No MeSH data available.


Related in: MedlinePlus

Schematic diagram of the experimental setup.
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iew047-F2: Schematic diagram of the experimental setup.

Mentions: The equipment set up for NIR imaging is shown in Fig. 2. Pupae were photographed under a dissection microscope (Stemi SR, Zeiss, Carl-Zeiss-Strasse 22, 73447 Oberkochen, Germany) using a USB eyepiece camera (Dino-Eye eyepiece camera, AM423X, AnMo Electronics Corp., Taiwan) with the infrared filter removed. The pupae were illuminated under LEDs of 880 nm (SFH 485-2, Osram Opto Semiconductors, Regensburg, Germany), 950 nm (TSAL6100, Vishay, Malvern, PA), 1060 nm (ELD-1060-525, EPIGAP Optronic GmbH, Berlin, Germany), and white (LTW-2S3D8, Lite-On Inc., Milpitas, CA) that were mounted on a circular stand in a ring around the pupa. The stand was made to fit inside the stage of the dissection microscope. The lights fit into holes inside a ring 30 mm above the base. The LEDs were arranged with one 880, 950, 1060 nm, and white LED placed in each of four equally spaced banks around the center of the stage. A rotary switch controlled which wavelength LEDs would illuminate: white, 880, 950 or 1060 nm infrared. An on/off switch was also installed for each of the four positions around the stand, so that any combination of the four could be illuminated. The LEDs were driven at 95 mA for the three NIR LEDs and 30 mA for the white LEDs using LM317LZ constant voltage sources as current drivers from a 7.5-V supply.Fig. 2.


Near Infrared Imaging As a Method of Studying Tsetse Fly (Diptera: Glossinidae) Pupal Development.

Moran ZR, Parker AG - J. Insect Sci. (2016)

Schematic diagram of the experimental setup.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940448&req=5

iew047-F2: Schematic diagram of the experimental setup.
Mentions: The equipment set up for NIR imaging is shown in Fig. 2. Pupae were photographed under a dissection microscope (Stemi SR, Zeiss, Carl-Zeiss-Strasse 22, 73447 Oberkochen, Germany) using a USB eyepiece camera (Dino-Eye eyepiece camera, AM423X, AnMo Electronics Corp., Taiwan) with the infrared filter removed. The pupae were illuminated under LEDs of 880 nm (SFH 485-2, Osram Opto Semiconductors, Regensburg, Germany), 950 nm (TSAL6100, Vishay, Malvern, PA), 1060 nm (ELD-1060-525, EPIGAP Optronic GmbH, Berlin, Germany), and white (LTW-2S3D8, Lite-On Inc., Milpitas, CA) that were mounted on a circular stand in a ring around the pupa. The stand was made to fit inside the stage of the dissection microscope. The lights fit into holes inside a ring 30 mm above the base. The LEDs were arranged with one 880, 950, 1060 nm, and white LED placed in each of four equally spaced banks around the center of the stage. A rotary switch controlled which wavelength LEDs would illuminate: white, 880, 950 or 1060 nm infrared. An on/off switch was also installed for each of the four positions around the stand, so that any combination of the four could be illuminated. The LEDs were driven at 95 mA for the three NIR LEDs and 30 mA for the white LEDs using LM317LZ constant voltage sources as current drivers from a 7.5-V supply.Fig. 2.

Bottom Line: Various wavelengths of NIR light from 880 to 1060 nm were compared to study the development of tsetse fly pupae from larviposition to emergence, using time-lapse videos and photographs.In addition, it presents a new methodology for studying the pupal stage of many coarctate insects for many applications.NIR imaging permits observation of living pupae, allowing the entire development process to be observed without disruption.

View Article: PubMed Central - PubMed

Affiliation: Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, P.O. Box 100, 1400 Vienna, Austria (zelda.moran@gmail.com; a.g.parker@iaea.org).

No MeSH data available.


Related in: MedlinePlus