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Identification of Putative RuBisCo Activase (TaRca1)-The Catalytic Chaperone Regulating Carbon Assimilatory Pathway in Wheat (Triticum aestivum) under the Heat Stress.

Kumar RR, Goswami S, Singh K, Dubey K, Singh S, Sharma R, Verma N, Kala YK, Rai GK, Grover M, Mishra DC, Singh B, Pathak H, Chinnusamy V, Rai A, Praveen S - Front Plant Sci (2016)

Bottom Line: HS causes decrease in the RuBisCo activity; maximum reduction was observed during pollination stage in thermosusceptible cvs. as validated through immunoblotting.We observed uniform carbon distribution in different tissues of thermotolerant cvs., as compared to thermosusceptible.There is, however, need to manipulate the thermal stability of TaRca1 enzyme through protein engineering for sustaining the photosynthetic rate under HS-a novel approach toward development of "climate-smart" crop.

View Article: PubMed Central - PubMed

Affiliation: Division of Biochemistry, Indian Agricultural Research Institute New Delhi, India.

ABSTRACT
RuBisCo activase (Rca) is a catalytic chaperone involved in modulating the activity of RuBisCo (key enzyme of photosynthetic pathway). Here, we identified eight novel transcripts from wheat through data mining predicted to be Rca and cloned a transcript of 1.4 kb from cv. HD2985, named as TaRca1 (GenBank acc. no. KC776912). Single copy number of TaRca1 was observed in wheat genome. Expression analysis in diverse wheat genotypes (HD2985, Halna, PBW621, and HD2329) showed very high relative expression of TaRca1 in Halna under control and HS-treated, as compared to other cultivars at different stages of growth. TaRca1 protein was predicted to be chloroplast-localized with numerous potential phosphorylation sites. Northern blot analysis showed maximum accumulation of TaRca1 transcript in thermotolerant cv. during mealy-ripe stage, as compared to thermosusceptible. Decrease in the photosynthetic parameters was observed in all the cultivars, except PBW621 in response to HS. We observed significant increase in the Rca activity in all the cultivars under HS at different stages of growth. HS causes decrease in the RuBisCo activity; maximum reduction was observed during pollination stage in thermosusceptible cvs. as validated through immunoblotting. We observed uniform carbon distribution in different tissues of thermotolerant cvs., as compared to thermosusceptible. Similarly, tolerance level of leaf was observed maximum in Halna having high Rca activity under HS. A positive correlation was observed between the transcript and activity of TaRca1 in HS-treated Halna. Similarly, TaRca1 enzyme showed positive correlation with the activity of RuBisCo. There is, however, need to manipulate the thermal stability of TaRca1 enzyme through protein engineering for sustaining the photosynthetic rate under HS-a novel approach toward development of "climate-smart" crop.

No MeSH data available.


Phylogenetic analysis of RuBisCo activase gene (TaRca1) cloned from wheat cv. HD2985. (A) Classification of TaRca1 based on RuBisCo activase reported from other plant and non-plant sources, (B) Classification based on Rca reported from Zea mays; (C) Classification based on Rca reported from Oryza sativa.
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Figure 1: Phylogenetic analysis of RuBisCo activase gene (TaRca1) cloned from wheat cv. HD2985. (A) Classification of TaRca1 based on RuBisCo activase reported from other plant and non-plant sources, (B) Classification based on Rca reported from Zea mays; (C) Classification based on Rca reported from Oryza sativa.

Mentions: Based on the phylogeny tree, the Rca was classified into four different sub-families with TaRca1 lying under subfamily—I (Figure 1A). The cloned TaRca1 was subjected to phylogeny analysis along with RuBisCo activase reported from Zea mays (Figure 1B). Based on the amino acid sequence analysis, the RuBisCo activase was broadly classified into two families—(a) Family-I and (b) Family-II, with family-II having three subfamilies; cloned TaRca1 was observed in subfamily-II of family-I. Similarly, Rca reported from Oryza sativa was used for the phylogeny analysis along with Rca reported from wheat (Figure 1C). Rca was classified into two families with family-II having four different subfamilies; cloned TaRca1 was observed in subfamily-II.


Identification of Putative RuBisCo Activase (TaRca1)-The Catalytic Chaperone Regulating Carbon Assimilatory Pathway in Wheat (Triticum aestivum) under the Heat Stress.

Kumar RR, Goswami S, Singh K, Dubey K, Singh S, Sharma R, Verma N, Kala YK, Rai GK, Grover M, Mishra DC, Singh B, Pathak H, Chinnusamy V, Rai A, Praveen S - Front Plant Sci (2016)

Phylogenetic analysis of RuBisCo activase gene (TaRca1) cloned from wheat cv. HD2985. (A) Classification of TaRca1 based on RuBisCo activase reported from other plant and non-plant sources, (B) Classification based on Rca reported from Zea mays; (C) Classification based on Rca reported from Oryza sativa.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940427&req=5

Figure 1: Phylogenetic analysis of RuBisCo activase gene (TaRca1) cloned from wheat cv. HD2985. (A) Classification of TaRca1 based on RuBisCo activase reported from other plant and non-plant sources, (B) Classification based on Rca reported from Zea mays; (C) Classification based on Rca reported from Oryza sativa.
Mentions: Based on the phylogeny tree, the Rca was classified into four different sub-families with TaRca1 lying under subfamily—I (Figure 1A). The cloned TaRca1 was subjected to phylogeny analysis along with RuBisCo activase reported from Zea mays (Figure 1B). Based on the amino acid sequence analysis, the RuBisCo activase was broadly classified into two families—(a) Family-I and (b) Family-II, with family-II having three subfamilies; cloned TaRca1 was observed in subfamily-II of family-I. Similarly, Rca reported from Oryza sativa was used for the phylogeny analysis along with Rca reported from wheat (Figure 1C). Rca was classified into two families with family-II having four different subfamilies; cloned TaRca1 was observed in subfamily-II.

Bottom Line: HS causes decrease in the RuBisCo activity; maximum reduction was observed during pollination stage in thermosusceptible cvs. as validated through immunoblotting.We observed uniform carbon distribution in different tissues of thermotolerant cvs., as compared to thermosusceptible.There is, however, need to manipulate the thermal stability of TaRca1 enzyme through protein engineering for sustaining the photosynthetic rate under HS-a novel approach toward development of "climate-smart" crop.

View Article: PubMed Central - PubMed

Affiliation: Division of Biochemistry, Indian Agricultural Research Institute New Delhi, India.

ABSTRACT
RuBisCo activase (Rca) is a catalytic chaperone involved in modulating the activity of RuBisCo (key enzyme of photosynthetic pathway). Here, we identified eight novel transcripts from wheat through data mining predicted to be Rca and cloned a transcript of 1.4 kb from cv. HD2985, named as TaRca1 (GenBank acc. no. KC776912). Single copy number of TaRca1 was observed in wheat genome. Expression analysis in diverse wheat genotypes (HD2985, Halna, PBW621, and HD2329) showed very high relative expression of TaRca1 in Halna under control and HS-treated, as compared to other cultivars at different stages of growth. TaRca1 protein was predicted to be chloroplast-localized with numerous potential phosphorylation sites. Northern blot analysis showed maximum accumulation of TaRca1 transcript in thermotolerant cv. during mealy-ripe stage, as compared to thermosusceptible. Decrease in the photosynthetic parameters was observed in all the cultivars, except PBW621 in response to HS. We observed significant increase in the Rca activity in all the cultivars under HS at different stages of growth. HS causes decrease in the RuBisCo activity; maximum reduction was observed during pollination stage in thermosusceptible cvs. as validated through immunoblotting. We observed uniform carbon distribution in different tissues of thermotolerant cvs., as compared to thermosusceptible. Similarly, tolerance level of leaf was observed maximum in Halna having high Rca activity under HS. A positive correlation was observed between the transcript and activity of TaRca1 in HS-treated Halna. Similarly, TaRca1 enzyme showed positive correlation with the activity of RuBisCo. There is, however, need to manipulate the thermal stability of TaRca1 enzyme through protein engineering for sustaining the photosynthetic rate under HS-a novel approach toward development of "climate-smart" crop.

No MeSH data available.