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Prenatal Stress, Fearfulness, and the Epigenome: Exploratory Analysis of Sex Differences in DNA Methylation of the Glucocorticoid Receptor Gene.

Ostlund BD, Conradt E, Crowell SE, Tyrka AR, Marsit CJ, Lester BM - Front Behav Neurosci (2016)

Bottom Line: We examined whether DNA methylation of the glucocorticoid receptor gene, NR3C1, was associated with experiences of stress by an expectant mother and fearfulness in her infant.In addition, increased methylation was significantly associated with greater fearfulness for females.Results suggest an experience-dependent pathway to fearfulness for female infants via epigenetic modification of the glucocorticoid receptor gene.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychology, University of Utah Salt Lake City, UT, USA.

ABSTRACT
Exposure to stress in utero is a risk factor for the development of problem behavior in the offspring, though precise pathways are unknown. We examined whether DNA methylation of the glucocorticoid receptor gene, NR3C1, was associated with experiences of stress by an expectant mother and fearfulness in her infant. Mothers reported on prenatal stress and infant temperament when infants were 5 months old (n = 68). Buccal cells for methylation analysis were collected from each infant. Prenatal stress was not related to infant fearfulness or NR3C1 methylation in the sample as a whole. Exploratory sex-specific analysis revealed a trend-level association between prenatal stress and increased methylation of NR3C1 exon 1F for female, but not male, infants. In addition, increased methylation was significantly associated with greater fearfulness for females. Results suggest an experience-dependent pathway to fearfulness for female infants via epigenetic modification of the glucocorticoid receptor gene. Future studies should examine prenatal stress in a comprehensive fashion while considering sex differences in epigenetic processes underlying infant temperament.

No MeSH data available.


Related in: MedlinePlus

Percent of DNA methylation of NR3C1 exon 1F for 5-month-old infants based on whether their mother experienced of a SLE during pregnancy.
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Figure 2: Percent of DNA methylation of NR3C1 exon 1F for 5-month-old infants based on whether their mother experienced of a SLE during pregnancy.

Mentions: Next, we examined the association between maternal SLE during pregnancy and methylation of NR3C1 at exon 1F. An independent samples t-test was conducted to determine if infants’ DNA methylation at 5 months differed based on prenatal exposure to maternal stress. There was no sample-wide effect, t(37.77) = −1.46, p = 0.15. Exploratory analyses testing for within sex effects showed that among females, DNA methylation was greater for infants whose mother experienced a SLE in pregnancy (M = 2.38, SD = 0.99) compared to those whose mothers did not (M = 1.85, SD = 0.41), t(21.41) = −2.01, p = 0.057, although this effect was trending toward significance. There was no effect for males, t(33) = −0.20, p = 0.84 (Figure 2).


Prenatal Stress, Fearfulness, and the Epigenome: Exploratory Analysis of Sex Differences in DNA Methylation of the Glucocorticoid Receptor Gene.

Ostlund BD, Conradt E, Crowell SE, Tyrka AR, Marsit CJ, Lester BM - Front Behav Neurosci (2016)

Percent of DNA methylation of NR3C1 exon 1F for 5-month-old infants based on whether their mother experienced of a SLE during pregnancy.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940423&req=5

Figure 2: Percent of DNA methylation of NR3C1 exon 1F for 5-month-old infants based on whether their mother experienced of a SLE during pregnancy.
Mentions: Next, we examined the association between maternal SLE during pregnancy and methylation of NR3C1 at exon 1F. An independent samples t-test was conducted to determine if infants’ DNA methylation at 5 months differed based on prenatal exposure to maternal stress. There was no sample-wide effect, t(37.77) = −1.46, p = 0.15. Exploratory analyses testing for within sex effects showed that among females, DNA methylation was greater for infants whose mother experienced a SLE in pregnancy (M = 2.38, SD = 0.99) compared to those whose mothers did not (M = 1.85, SD = 0.41), t(21.41) = −2.01, p = 0.057, although this effect was trending toward significance. There was no effect for males, t(33) = −0.20, p = 0.84 (Figure 2).

Bottom Line: We examined whether DNA methylation of the glucocorticoid receptor gene, NR3C1, was associated with experiences of stress by an expectant mother and fearfulness in her infant.In addition, increased methylation was significantly associated with greater fearfulness for females.Results suggest an experience-dependent pathway to fearfulness for female infants via epigenetic modification of the glucocorticoid receptor gene.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychology, University of Utah Salt Lake City, UT, USA.

ABSTRACT
Exposure to stress in utero is a risk factor for the development of problem behavior in the offspring, though precise pathways are unknown. We examined whether DNA methylation of the glucocorticoid receptor gene, NR3C1, was associated with experiences of stress by an expectant mother and fearfulness in her infant. Mothers reported on prenatal stress and infant temperament when infants were 5 months old (n = 68). Buccal cells for methylation analysis were collected from each infant. Prenatal stress was not related to infant fearfulness or NR3C1 methylation in the sample as a whole. Exploratory sex-specific analysis revealed a trend-level association between prenatal stress and increased methylation of NR3C1 exon 1F for female, but not male, infants. In addition, increased methylation was significantly associated with greater fearfulness for females. Results suggest an experience-dependent pathway to fearfulness for female infants via epigenetic modification of the glucocorticoid receptor gene. Future studies should examine prenatal stress in a comprehensive fashion while considering sex differences in epigenetic processes underlying infant temperament.

No MeSH data available.


Related in: MedlinePlus