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Assessing Fungal Population in Soil Planted with Cry1Ac and CPTI Transgenic Cotton and Its Conventional Parental Line Using 18S and ITS rDNA Sequences over Four Seasons.

Qi X, Liu B, Song Q, Zou B, Bu Y, Wu H, Ding L, Zhou G - Front Plant Sci (2016)

Bottom Line: Long-term growth of genetically modified plants (GMPs) has raised concerns regarding their ecological effects.Overall, we conclude that monoculture of one representative transgenic cotton cultivar may have no effect on fungal diversity compared with conventional cotton.Furthermore, the choice of amplified region and methodology has potential to affect the outcome of the comparison between GM-crop and its parental line.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Jinling Hospital, State Key Laboratory of Analytical Chemistry for Life Science, School of Medicine, Nanjing UniversityNanjing, China; Department of Pharmaceutical Analysis, China Pharmaceutical UniversityNanjing, China.

ABSTRACT
Long-term growth of genetically modified plants (GMPs) has raised concerns regarding their ecological effects. Here, FLX-pyrosequencing of region I (18S) and region II (ITS1, 5.8S, and ITS2) rDNA was used to characterize fungal communities in soil samples after 10-year monoculture of one representative transgenic cotton line (TC-10) and 15-year plantation of various transgenic cotton cultivars (TC-15mix) over four seasons. Soil fungal communities in the rhizosphere of non-transgenic control (CC) were also compared. No notable differences were observed in soil fertility variables among CC, TC-10, and TC-15mix. Within seasons, the different estimations were statistically indistinguishable. There were 411 and 2 067 fungal operational taxonomic units in the two regions, respectively. More than 75% of fungal taxa were stable in both CC and TC except for individual taxa with significantly different abundance between TC and CC. Statistical analysis revealed no significant differences between CC and TC-10, while discrimination of separating TC-15mix from CC and TC-10 with 37.86% explained variance in PCoA and a significant difference of Shannon indexes between TC-10 and TC-15mix were observed in region II. As TC-15mix planted with a mixture of transgenic cottons (Zhongmian-29, 30, and 33B) for over 5 years, different genetic modifications may introduce variations in fungal diversity. Further clarification is necessary by detecting the fungal dynamic changes in sites planted in monoculture of various transgenic cottons. Overall, we conclude that monoculture of one representative transgenic cotton cultivar may have no effect on fungal diversity compared with conventional cotton. Furthermore, the choice of amplified region and methodology has potential to affect the outcome of the comparison between GM-crop and its parental line.

No MeSH data available.


Principal coordinate analysis of soil samples by weighted UniFrac. (A) Region I; (B) Region II.
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Figure 4: Principal coordinate analysis of soil samples by weighted UniFrac. (A) Region I; (B) Region II.

Mentions: The similarity of the 47 samples in region I and 48 samples in region II were evaluated by cluster analysis and principle coordinate analysis (PCoA), respectively. Based on the fungal communities, the soil samples could be clustered into several groups in cluster analysis (Supplementary Figure S2). An ambiguous cluster version among samples from CC, TC-10, and TC-15mix was shown in region I. In region II almost all samples from CC separated from those in TC-15mix except for No.33 (branch IV) and 31 (branch II), while CC and TC-10 clustered together. PCoA was also performed to elucidate similarities among different soil samples based on OTUs and exhibited similar observations with cluster analysis. As shown in Figure 4 (UniFrac at a 3% cutoff), no obvious clusters among the soil samples were observed in region I. PC1 axes separated TC-15mix from CC and TC-10 with 37.86% explained variance in region II.


Assessing Fungal Population in Soil Planted with Cry1Ac and CPTI Transgenic Cotton and Its Conventional Parental Line Using 18S and ITS rDNA Sequences over Four Seasons.

Qi X, Liu B, Song Q, Zou B, Bu Y, Wu H, Ding L, Zhou G - Front Plant Sci (2016)

Principal coordinate analysis of soil samples by weighted UniFrac. (A) Region I; (B) Region II.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940383&req=5

Figure 4: Principal coordinate analysis of soil samples by weighted UniFrac. (A) Region I; (B) Region II.
Mentions: The similarity of the 47 samples in region I and 48 samples in region II were evaluated by cluster analysis and principle coordinate analysis (PCoA), respectively. Based on the fungal communities, the soil samples could be clustered into several groups in cluster analysis (Supplementary Figure S2). An ambiguous cluster version among samples from CC, TC-10, and TC-15mix was shown in region I. In region II almost all samples from CC separated from those in TC-15mix except for No.33 (branch IV) and 31 (branch II), while CC and TC-10 clustered together. PCoA was also performed to elucidate similarities among different soil samples based on OTUs and exhibited similar observations with cluster analysis. As shown in Figure 4 (UniFrac at a 3% cutoff), no obvious clusters among the soil samples were observed in region I. PC1 axes separated TC-15mix from CC and TC-10 with 37.86% explained variance in region II.

Bottom Line: Long-term growth of genetically modified plants (GMPs) has raised concerns regarding their ecological effects.Overall, we conclude that monoculture of one representative transgenic cotton cultivar may have no effect on fungal diversity compared with conventional cotton.Furthermore, the choice of amplified region and methodology has potential to affect the outcome of the comparison between GM-crop and its parental line.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Jinling Hospital, State Key Laboratory of Analytical Chemistry for Life Science, School of Medicine, Nanjing UniversityNanjing, China; Department of Pharmaceutical Analysis, China Pharmaceutical UniversityNanjing, China.

ABSTRACT
Long-term growth of genetically modified plants (GMPs) has raised concerns regarding their ecological effects. Here, FLX-pyrosequencing of region I (18S) and region II (ITS1, 5.8S, and ITS2) rDNA was used to characterize fungal communities in soil samples after 10-year monoculture of one representative transgenic cotton line (TC-10) and 15-year plantation of various transgenic cotton cultivars (TC-15mix) over four seasons. Soil fungal communities in the rhizosphere of non-transgenic control (CC) were also compared. No notable differences were observed in soil fertility variables among CC, TC-10, and TC-15mix. Within seasons, the different estimations were statistically indistinguishable. There were 411 and 2 067 fungal operational taxonomic units in the two regions, respectively. More than 75% of fungal taxa were stable in both CC and TC except for individual taxa with significantly different abundance between TC and CC. Statistical analysis revealed no significant differences between CC and TC-10, while discrimination of separating TC-15mix from CC and TC-10 with 37.86% explained variance in PCoA and a significant difference of Shannon indexes between TC-10 and TC-15mix were observed in region II. As TC-15mix planted with a mixture of transgenic cottons (Zhongmian-29, 30, and 33B) for over 5 years, different genetic modifications may introduce variations in fungal diversity. Further clarification is necessary by detecting the fungal dynamic changes in sites planted in monoculture of various transgenic cottons. Overall, we conclude that monoculture of one representative transgenic cotton cultivar may have no effect on fungal diversity compared with conventional cotton. Furthermore, the choice of amplified region and methodology has potential to affect the outcome of the comparison between GM-crop and its parental line.

No MeSH data available.