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Assessing Fungal Population in Soil Planted with Cry1Ac and CPTI Transgenic Cotton and Its Conventional Parental Line Using 18S and ITS rDNA Sequences over Four Seasons.

Qi X, Liu B, Song Q, Zou B, Bu Y, Wu H, Ding L, Zhou G - Front Plant Sci (2016)

Bottom Line: Long-term growth of genetically modified plants (GMPs) has raised concerns regarding their ecological effects.Overall, we conclude that monoculture of one representative transgenic cotton cultivar may have no effect on fungal diversity compared with conventional cotton.Furthermore, the choice of amplified region and methodology has potential to affect the outcome of the comparison between GM-crop and its parental line.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Jinling Hospital, State Key Laboratory of Analytical Chemistry for Life Science, School of Medicine, Nanjing UniversityNanjing, China; Department of Pharmaceutical Analysis, China Pharmaceutical UniversityNanjing, China.

ABSTRACT
Long-term growth of genetically modified plants (GMPs) has raised concerns regarding their ecological effects. Here, FLX-pyrosequencing of region I (18S) and region II (ITS1, 5.8S, and ITS2) rDNA was used to characterize fungal communities in soil samples after 10-year monoculture of one representative transgenic cotton line (TC-10) and 15-year plantation of various transgenic cotton cultivars (TC-15mix) over four seasons. Soil fungal communities in the rhizosphere of non-transgenic control (CC) were also compared. No notable differences were observed in soil fertility variables among CC, TC-10, and TC-15mix. Within seasons, the different estimations were statistically indistinguishable. There were 411 and 2 067 fungal operational taxonomic units in the two regions, respectively. More than 75% of fungal taxa were stable in both CC and TC except for individual taxa with significantly different abundance between TC and CC. Statistical analysis revealed no significant differences between CC and TC-10, while discrimination of separating TC-15mix from CC and TC-10 with 37.86% explained variance in PCoA and a significant difference of Shannon indexes between TC-10 and TC-15mix were observed in region II. As TC-15mix planted with a mixture of transgenic cottons (Zhongmian-29, 30, and 33B) for over 5 years, different genetic modifications may introduce variations in fungal diversity. Further clarification is necessary by detecting the fungal dynamic changes in sites planted in monoculture of various transgenic cottons. Overall, we conclude that monoculture of one representative transgenic cotton cultivar may have no effect on fungal diversity compared with conventional cotton. Furthermore, the choice of amplified region and methodology has potential to affect the outcome of the comparison between GM-crop and its parental line.

No MeSH data available.


Significant differences among CC, TC-10, and TC-15mix at the genus level during different seasons (P < 0.05). (A) Region I; (B) Region II. The graphs show the levels for genera with frequencies ≥30 in all samples (48 for region I and 47 for region II) and abundances ≥0.01%. S: seeding stage (26 April); B: bud stage (13 July); Bl: blooming stage (22 August); Bo: boll opening stage (17 October) in 2011. ∗P < 0.05 among the four seasons in the corresponding site. The double asterisks represent significant difference with a P-value of <0.01.
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Figure 3: Significant differences among CC, TC-10, and TC-15mix at the genus level during different seasons (P < 0.05). (A) Region I; (B) Region II. The graphs show the levels for genera with frequencies ≥30 in all samples (48 for region I and 47 for region II) and abundances ≥0.01%. S: seeding stage (26 April); B: bud stage (13 July); Bl: blooming stage (22 August); Bo: boll opening stage (17 October) in 2011. ∗P < 0.05 among the four seasons in the corresponding site. The double asterisks represent significant difference with a P-value of <0.01.

Mentions: The abundance of rare genera specific to CC, TC-10, or TC-15mix was less than 0.065%. Some individual genera with significant differences in abundance among CC, TC-10, and TC-15mix over four seasons (lines with different colors) were shown in Figure 3. Both regions contained the genera Geomyces and Poitrasia, and importantly, each season provided a similar pattern of abundance among CC, TC-10, and TC-15mix. The present study also showed that the abundance of Alternaria (region II) and Chaetomium (region I) decreased by approximately 30% on average in TC-10 in comparison to CC in all four sampling periods and increased to the same level as CC in TC-15mix. In contrast, for Poitrasia (in both regions) and Petalosphaeria (region I) at the blooming stage, the abundance increased significantly (approximately 60-fold) in TC-10 compared with CC and decreased to the same level as CC in TC-15mix. Although the abundance of some genera showed significant difference between CC and TC, the proportion of them was less than 10%.


Assessing Fungal Population in Soil Planted with Cry1Ac and CPTI Transgenic Cotton and Its Conventional Parental Line Using 18S and ITS rDNA Sequences over Four Seasons.

Qi X, Liu B, Song Q, Zou B, Bu Y, Wu H, Ding L, Zhou G - Front Plant Sci (2016)

Significant differences among CC, TC-10, and TC-15mix at the genus level during different seasons (P < 0.05). (A) Region I; (B) Region II. The graphs show the levels for genera with frequencies ≥30 in all samples (48 for region I and 47 for region II) and abundances ≥0.01%. S: seeding stage (26 April); B: bud stage (13 July); Bl: blooming stage (22 August); Bo: boll opening stage (17 October) in 2011. ∗P < 0.05 among the four seasons in the corresponding site. The double asterisks represent significant difference with a P-value of <0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940383&req=5

Figure 3: Significant differences among CC, TC-10, and TC-15mix at the genus level during different seasons (P < 0.05). (A) Region I; (B) Region II. The graphs show the levels for genera with frequencies ≥30 in all samples (48 for region I and 47 for region II) and abundances ≥0.01%. S: seeding stage (26 April); B: bud stage (13 July); Bl: blooming stage (22 August); Bo: boll opening stage (17 October) in 2011. ∗P < 0.05 among the four seasons in the corresponding site. The double asterisks represent significant difference with a P-value of <0.01.
Mentions: The abundance of rare genera specific to CC, TC-10, or TC-15mix was less than 0.065%. Some individual genera with significant differences in abundance among CC, TC-10, and TC-15mix over four seasons (lines with different colors) were shown in Figure 3. Both regions contained the genera Geomyces and Poitrasia, and importantly, each season provided a similar pattern of abundance among CC, TC-10, and TC-15mix. The present study also showed that the abundance of Alternaria (region II) and Chaetomium (region I) decreased by approximately 30% on average in TC-10 in comparison to CC in all four sampling periods and increased to the same level as CC in TC-15mix. In contrast, for Poitrasia (in both regions) and Petalosphaeria (region I) at the blooming stage, the abundance increased significantly (approximately 60-fold) in TC-10 compared with CC and decreased to the same level as CC in TC-15mix. Although the abundance of some genera showed significant difference between CC and TC, the proportion of them was less than 10%.

Bottom Line: Long-term growth of genetically modified plants (GMPs) has raised concerns regarding their ecological effects.Overall, we conclude that monoculture of one representative transgenic cotton cultivar may have no effect on fungal diversity compared with conventional cotton.Furthermore, the choice of amplified region and methodology has potential to affect the outcome of the comparison between GM-crop and its parental line.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Jinling Hospital, State Key Laboratory of Analytical Chemistry for Life Science, School of Medicine, Nanjing UniversityNanjing, China; Department of Pharmaceutical Analysis, China Pharmaceutical UniversityNanjing, China.

ABSTRACT
Long-term growth of genetically modified plants (GMPs) has raised concerns regarding their ecological effects. Here, FLX-pyrosequencing of region I (18S) and region II (ITS1, 5.8S, and ITS2) rDNA was used to characterize fungal communities in soil samples after 10-year monoculture of one representative transgenic cotton line (TC-10) and 15-year plantation of various transgenic cotton cultivars (TC-15mix) over four seasons. Soil fungal communities in the rhizosphere of non-transgenic control (CC) were also compared. No notable differences were observed in soil fertility variables among CC, TC-10, and TC-15mix. Within seasons, the different estimations were statistically indistinguishable. There were 411 and 2 067 fungal operational taxonomic units in the two regions, respectively. More than 75% of fungal taxa were stable in both CC and TC except for individual taxa with significantly different abundance between TC and CC. Statistical analysis revealed no significant differences between CC and TC-10, while discrimination of separating TC-15mix from CC and TC-10 with 37.86% explained variance in PCoA and a significant difference of Shannon indexes between TC-10 and TC-15mix were observed in region II. As TC-15mix planted with a mixture of transgenic cottons (Zhongmian-29, 30, and 33B) for over 5 years, different genetic modifications may introduce variations in fungal diversity. Further clarification is necessary by detecting the fungal dynamic changes in sites planted in monoculture of various transgenic cottons. Overall, we conclude that monoculture of one representative transgenic cotton cultivar may have no effect on fungal diversity compared with conventional cotton. Furthermore, the choice of amplified region and methodology has potential to affect the outcome of the comparison between GM-crop and its parental line.

No MeSH data available.