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Assessing Fungal Population in Soil Planted with Cry1Ac and CPTI Transgenic Cotton and Its Conventional Parental Line Using 18S and ITS rDNA Sequences over Four Seasons.

Qi X, Liu B, Song Q, Zou B, Bu Y, Wu H, Ding L, Zhou G - Front Plant Sci (2016)

Bottom Line: Long-term growth of genetically modified plants (GMPs) has raised concerns regarding their ecological effects.Overall, we conclude that monoculture of one representative transgenic cotton cultivar may have no effect on fungal diversity compared with conventional cotton.Furthermore, the choice of amplified region and methodology has potential to affect the outcome of the comparison between GM-crop and its parental line.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Jinling Hospital, State Key Laboratory of Analytical Chemistry for Life Science, School of Medicine, Nanjing UniversityNanjing, China; Department of Pharmaceutical Analysis, China Pharmaceutical UniversityNanjing, China.

ABSTRACT
Long-term growth of genetically modified plants (GMPs) has raised concerns regarding their ecological effects. Here, FLX-pyrosequencing of region I (18S) and region II (ITS1, 5.8S, and ITS2) rDNA was used to characterize fungal communities in soil samples after 10-year monoculture of one representative transgenic cotton line (TC-10) and 15-year plantation of various transgenic cotton cultivars (TC-15mix) over four seasons. Soil fungal communities in the rhizosphere of non-transgenic control (CC) were also compared. No notable differences were observed in soil fertility variables among CC, TC-10, and TC-15mix. Within seasons, the different estimations were statistically indistinguishable. There were 411 and 2 067 fungal operational taxonomic units in the two regions, respectively. More than 75% of fungal taxa were stable in both CC and TC except for individual taxa with significantly different abundance between TC and CC. Statistical analysis revealed no significant differences between CC and TC-10, while discrimination of separating TC-15mix from CC and TC-10 with 37.86% explained variance in PCoA and a significant difference of Shannon indexes between TC-10 and TC-15mix were observed in region II. As TC-15mix planted with a mixture of transgenic cottons (Zhongmian-29, 30, and 33B) for over 5 years, different genetic modifications may introduce variations in fungal diversity. Further clarification is necessary by detecting the fungal dynamic changes in sites planted in monoculture of various transgenic cottons. Overall, we conclude that monoculture of one representative transgenic cotton cultivar may have no effect on fungal diversity compared with conventional cotton. Furthermore, the choice of amplified region and methodology has potential to affect the outcome of the comparison between GM-crop and its parental line.

No MeSH data available.


Relative abundance (%) of fungal phyla in the overall communities of soil samples from different groups (CC, TC-10, and TC-15mix) in region I (A) and region II (B). (A) Others include Alveolata, Metazoa, Unclassified_eukaryota, Viridiplantae, and Stramenopiles. (B) Others include Algae, Alveolata, Amoebozoa, Cryptophyta, Euglenozoa, Metazoa, Viridiplantae, and Unclassified_eukaryota. ∗Significant difference with a P-value of <0.05 in comparison with CC; ∗∗Significant difference with a P-value of <0.01 in comparison with CC. ΔSignificant difference with a P-value of <0.05 in comparison with TC-10; ΔΔSignificant difference with a P-value of <0.01 in comparison with TC-10.
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Figure 1: Relative abundance (%) of fungal phyla in the overall communities of soil samples from different groups (CC, TC-10, and TC-15mix) in region I (A) and region II (B). (A) Others include Alveolata, Metazoa, Unclassified_eukaryota, Viridiplantae, and Stramenopiles. (B) Others include Algae, Alveolata, Amoebozoa, Cryptophyta, Euglenozoa, Metazoa, Viridiplantae, and Unclassified_eukaryota. ∗Significant difference with a P-value of <0.05 in comparison with CC; ∗∗Significant difference with a P-value of <0.01 in comparison with CC. ΔSignificant difference with a P-value of <0.05 in comparison with TC-10; ΔΔSignificant difference with a P-value of <0.01 in comparison with TC-10.

Mentions: Figure 1 shows the relative abundance of the fungal phyla, unclassified fungi and non-fungi (others) in the overall communities of the soil samples from different groups (CC, TC-10, and TC-15mix) in region I and region II, respectively. In region I (Figure 1A), excluding the non-fungi (others), Ascomycota represented the dominant lineage in each group, accounting for 58, 62, and 69% of all sequences in the CC, TC-10, and TC-15mix groups, respectively. Fungi_incertae_sedis accounted for 1.0 and 1.4% of the CC and TC-10 groups, respectively, but decreased to 0.16% in the TC-15mix group. The relative abundance of Basidiomycota and Glomeromycota were 0.12% and 0.13% in TC-15mix, respectively, but reduced to 0.018% and 0.013% in CC, and to 0.014% and 0.022% in TC-10. These data suggested that the relative abundance of Fungi_incertae_sedis, Basidiomycota and Glomeromycota varied largely in the CC, TC-10, and TC-15mix groups and that the relative abundance of the unclassified fungi was nearly equivalent among the samples from the three groups.


Assessing Fungal Population in Soil Planted with Cry1Ac and CPTI Transgenic Cotton and Its Conventional Parental Line Using 18S and ITS rDNA Sequences over Four Seasons.

Qi X, Liu B, Song Q, Zou B, Bu Y, Wu H, Ding L, Zhou G - Front Plant Sci (2016)

Relative abundance (%) of fungal phyla in the overall communities of soil samples from different groups (CC, TC-10, and TC-15mix) in region I (A) and region II (B). (A) Others include Alveolata, Metazoa, Unclassified_eukaryota, Viridiplantae, and Stramenopiles. (B) Others include Algae, Alveolata, Amoebozoa, Cryptophyta, Euglenozoa, Metazoa, Viridiplantae, and Unclassified_eukaryota. ∗Significant difference with a P-value of <0.05 in comparison with CC; ∗∗Significant difference with a P-value of <0.01 in comparison with CC. ΔSignificant difference with a P-value of <0.05 in comparison with TC-10; ΔΔSignificant difference with a P-value of <0.01 in comparison with TC-10.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940383&req=5

Figure 1: Relative abundance (%) of fungal phyla in the overall communities of soil samples from different groups (CC, TC-10, and TC-15mix) in region I (A) and region II (B). (A) Others include Alveolata, Metazoa, Unclassified_eukaryota, Viridiplantae, and Stramenopiles. (B) Others include Algae, Alveolata, Amoebozoa, Cryptophyta, Euglenozoa, Metazoa, Viridiplantae, and Unclassified_eukaryota. ∗Significant difference with a P-value of <0.05 in comparison with CC; ∗∗Significant difference with a P-value of <0.01 in comparison with CC. ΔSignificant difference with a P-value of <0.05 in comparison with TC-10; ΔΔSignificant difference with a P-value of <0.01 in comparison with TC-10.
Mentions: Figure 1 shows the relative abundance of the fungal phyla, unclassified fungi and non-fungi (others) in the overall communities of the soil samples from different groups (CC, TC-10, and TC-15mix) in region I and region II, respectively. In region I (Figure 1A), excluding the non-fungi (others), Ascomycota represented the dominant lineage in each group, accounting for 58, 62, and 69% of all sequences in the CC, TC-10, and TC-15mix groups, respectively. Fungi_incertae_sedis accounted for 1.0 and 1.4% of the CC and TC-10 groups, respectively, but decreased to 0.16% in the TC-15mix group. The relative abundance of Basidiomycota and Glomeromycota were 0.12% and 0.13% in TC-15mix, respectively, but reduced to 0.018% and 0.013% in CC, and to 0.014% and 0.022% in TC-10. These data suggested that the relative abundance of Fungi_incertae_sedis, Basidiomycota and Glomeromycota varied largely in the CC, TC-10, and TC-15mix groups and that the relative abundance of the unclassified fungi was nearly equivalent among the samples from the three groups.

Bottom Line: Long-term growth of genetically modified plants (GMPs) has raised concerns regarding their ecological effects.Overall, we conclude that monoculture of one representative transgenic cotton cultivar may have no effect on fungal diversity compared with conventional cotton.Furthermore, the choice of amplified region and methodology has potential to affect the outcome of the comparison between GM-crop and its parental line.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Jinling Hospital, State Key Laboratory of Analytical Chemistry for Life Science, School of Medicine, Nanjing UniversityNanjing, China; Department of Pharmaceutical Analysis, China Pharmaceutical UniversityNanjing, China.

ABSTRACT
Long-term growth of genetically modified plants (GMPs) has raised concerns regarding their ecological effects. Here, FLX-pyrosequencing of region I (18S) and region II (ITS1, 5.8S, and ITS2) rDNA was used to characterize fungal communities in soil samples after 10-year monoculture of one representative transgenic cotton line (TC-10) and 15-year plantation of various transgenic cotton cultivars (TC-15mix) over four seasons. Soil fungal communities in the rhizosphere of non-transgenic control (CC) were also compared. No notable differences were observed in soil fertility variables among CC, TC-10, and TC-15mix. Within seasons, the different estimations were statistically indistinguishable. There were 411 and 2 067 fungal operational taxonomic units in the two regions, respectively. More than 75% of fungal taxa were stable in both CC and TC except for individual taxa with significantly different abundance between TC and CC. Statistical analysis revealed no significant differences between CC and TC-10, while discrimination of separating TC-15mix from CC and TC-10 with 37.86% explained variance in PCoA and a significant difference of Shannon indexes between TC-10 and TC-15mix were observed in region II. As TC-15mix planted with a mixture of transgenic cottons (Zhongmian-29, 30, and 33B) for over 5 years, different genetic modifications may introduce variations in fungal diversity. Further clarification is necessary by detecting the fungal dynamic changes in sites planted in monoculture of various transgenic cottons. Overall, we conclude that monoculture of one representative transgenic cotton cultivar may have no effect on fungal diversity compared with conventional cotton. Furthermore, the choice of amplified region and methodology has potential to affect the outcome of the comparison between GM-crop and its parental line.

No MeSH data available.