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Antimicrobial Activities of Bacteria Associated with the Brown Alga Padina pavonica.

Ismail A, Ktari L, Ahmed M, Bolhuis H, Boudabbous A, Stal LJ, Cretoiu MS, El Bour M - Front Microbiol (2016)

Bottom Line: Eighteen isolates were obtained in pure culture and tested for antimicrobial activities.Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates).The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Marine Sciences and Technologies Salammb├┤, Tunisia.

ABSTRACT
Macroalgae belonging to the genus Padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. Hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by secondary metabolite producing epiphytic bacteria. Here we report antibacterial activities of epiphytic bacteria isolated from Padina pavonica (Peacocks tail) located on northern coast of Tunisia. Eighteen isolates were obtained in pure culture and tested for antimicrobial activities. Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates). The antimicrobial activity was assessed as inhibition of growth of 12 species of pathogenic bacteria (Aeromonas salmonicida, A. hydrophila, Enterobacter xiangfangensis, Enterococcus faecium, Escherichia coli, Micrococcus sp., Salmonella typhimurium, Staphylococcus aureus, Streptococcus sp., Vibrio alginoliticus, V. proteolyticus, V. vulnificus) and one pathogenic yeast (Candida albicans). Among the Firmicutes, isolate P8, which is closely related to Bacillus pumilus, displayed the largest spectrum of growth inhibition of the pathogenic bacteria tested. The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

No MeSH data available.


Related in: MedlinePlus

Comparison between P. pavonica crude extracts inhibition spectrum against sensitive alga isolates and pathogenic human and fish sensitive bacteria (18 pathogens).
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Figure 4: Comparison between P. pavonica crude extracts inhibition spectrum against sensitive alga isolates and pathogenic human and fish sensitive bacteria (18 pathogens).

Mentions: P. pavonica harbors several culturable epiphytic bacteria on its surface and our harsh isolation procedure indicates that these epibionts are intimately associated with their host. We tested the resistance of the isolates to crude extracts of the P. pavonica. Only extracts from P. pavonica harvested in summer revealed a low inhibitory effect toward three of the isolates. Organic crude extracts of P. pavonica were toxic against human and fish pathogens such as V. tapetis, V. anguillarum, V. alginoliticus, Ps. cepacia, A. salmonicida, A. hydrophila, E. faecalis ATCC 29212, and S. aureus (Ismail-Ben Ali et al., 2010a). In Figure 4 a diagram is depicted showing the number of pathogenic bacteria inhibited by P. pavonica extracts, which represent 50% of the total number of pathogens tested (Ismail-Ben Ali et al., 2010a), compared to P. pavonica isolates that are inhibited by their host extracts, which represent only 16.6% of the total isolates tested. Chbani et al. (2011) and Zerrin et al. (2011) also reported toxicity of P. pavonica against pathogenic indicator bacteria.


Antimicrobial Activities of Bacteria Associated with the Brown Alga Padina pavonica.

Ismail A, Ktari L, Ahmed M, Bolhuis H, Boudabbous A, Stal LJ, Cretoiu MS, El Bour M - Front Microbiol (2016)

Comparison between P. pavonica crude extracts inhibition spectrum against sensitive alga isolates and pathogenic human and fish sensitive bacteria (18 pathogens).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940378&req=5

Figure 4: Comparison between P. pavonica crude extracts inhibition spectrum against sensitive alga isolates and pathogenic human and fish sensitive bacteria (18 pathogens).
Mentions: P. pavonica harbors several culturable epiphytic bacteria on its surface and our harsh isolation procedure indicates that these epibionts are intimately associated with their host. We tested the resistance of the isolates to crude extracts of the P. pavonica. Only extracts from P. pavonica harvested in summer revealed a low inhibitory effect toward three of the isolates. Organic crude extracts of P. pavonica were toxic against human and fish pathogens such as V. tapetis, V. anguillarum, V. alginoliticus, Ps. cepacia, A. salmonicida, A. hydrophila, E. faecalis ATCC 29212, and S. aureus (Ismail-Ben Ali et al., 2010a). In Figure 4 a diagram is depicted showing the number of pathogenic bacteria inhibited by P. pavonica extracts, which represent 50% of the total number of pathogens tested (Ismail-Ben Ali et al., 2010a), compared to P. pavonica isolates that are inhibited by their host extracts, which represent only 16.6% of the total isolates tested. Chbani et al. (2011) and Zerrin et al. (2011) also reported toxicity of P. pavonica against pathogenic indicator bacteria.

Bottom Line: Eighteen isolates were obtained in pure culture and tested for antimicrobial activities.Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates).The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Marine Sciences and Technologies Salammb├┤, Tunisia.

ABSTRACT
Macroalgae belonging to the genus Padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. Hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by secondary metabolite producing epiphytic bacteria. Here we report antibacterial activities of epiphytic bacteria isolated from Padina pavonica (Peacocks tail) located on northern coast of Tunisia. Eighteen isolates were obtained in pure culture and tested for antimicrobial activities. Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates). The antimicrobial activity was assessed as inhibition of growth of 12 species of pathogenic bacteria (Aeromonas salmonicida, A. hydrophila, Enterobacter xiangfangensis, Enterococcus faecium, Escherichia coli, Micrococcus sp., Salmonella typhimurium, Staphylococcus aureus, Streptococcus sp., Vibrio alginoliticus, V. proteolyticus, V. vulnificus) and one pathogenic yeast (Candida albicans). Among the Firmicutes, isolate P8, which is closely related to Bacillus pumilus, displayed the largest spectrum of growth inhibition of the pathogenic bacteria tested. The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

No MeSH data available.


Related in: MedlinePlus