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Antimicrobial Activities of Bacteria Associated with the Brown Alga Padina pavonica.

Ismail A, Ktari L, Ahmed M, Bolhuis H, Boudabbous A, Stal LJ, Cretoiu MS, El Bour M - Front Microbiol (2016)

Bottom Line: Eighteen isolates were obtained in pure culture and tested for antimicrobial activities.Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates).The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Marine Sciences and Technologies Salammbô, Tunisia.

ABSTRACT
Macroalgae belonging to the genus Padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. Hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by secondary metabolite producing epiphytic bacteria. Here we report antibacterial activities of epiphytic bacteria isolated from Padina pavonica (Peacocks tail) located on northern coast of Tunisia. Eighteen isolates were obtained in pure culture and tested for antimicrobial activities. Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates). The antimicrobial activity was assessed as inhibition of growth of 12 species of pathogenic bacteria (Aeromonas salmonicida, A. hydrophila, Enterobacter xiangfangensis, Enterococcus faecium, Escherichia coli, Micrococcus sp., Salmonella typhimurium, Staphylococcus aureus, Streptococcus sp., Vibrio alginoliticus, V. proteolyticus, V. vulnificus) and one pathogenic yeast (Candida albicans). Among the Firmicutes, isolate P8, which is closely related to Bacillus pumilus, displayed the largest spectrum of growth inhibition of the pathogenic bacteria tested. The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

No MeSH data available.


Related in: MedlinePlus

Influence of supernatant heating treatment on the P8 antibacterial activity against Staphylococcus aureus and Micrococcus sp.
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Figure 3: Influence of supernatant heating treatment on the P8 antibacterial activity against Staphylococcus aureus and Micrococcus sp.

Mentions: The active substance produced by isolate P8 was more resistant to increased temperatures since activity was not completely lost at 60°C but decreased with increasing temperature. About 50% of the diffusion zone remained after incubation at 60°C and 20% at 80°C, while the activity was totally lost after a treatment for 5 min at 100°C (Figure 3).


Antimicrobial Activities of Bacteria Associated with the Brown Alga Padina pavonica.

Ismail A, Ktari L, Ahmed M, Bolhuis H, Boudabbous A, Stal LJ, Cretoiu MS, El Bour M - Front Microbiol (2016)

Influence of supernatant heating treatment on the P8 antibacterial activity against Staphylococcus aureus and Micrococcus sp.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940378&req=5

Figure 3: Influence of supernatant heating treatment on the P8 antibacterial activity against Staphylococcus aureus and Micrococcus sp.
Mentions: The active substance produced by isolate P8 was more resistant to increased temperatures since activity was not completely lost at 60°C but decreased with increasing temperature. About 50% of the diffusion zone remained after incubation at 60°C and 20% at 80°C, while the activity was totally lost after a treatment for 5 min at 100°C (Figure 3).

Bottom Line: Eighteen isolates were obtained in pure culture and tested for antimicrobial activities.Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates).The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Marine Sciences and Technologies Salammbô, Tunisia.

ABSTRACT
Macroalgae belonging to the genus Padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. Hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by secondary metabolite producing epiphytic bacteria. Here we report antibacterial activities of epiphytic bacteria isolated from Padina pavonica (Peacocks tail) located on northern coast of Tunisia. Eighteen isolates were obtained in pure culture and tested for antimicrobial activities. Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates). The antimicrobial activity was assessed as inhibition of growth of 12 species of pathogenic bacteria (Aeromonas salmonicida, A. hydrophila, Enterobacter xiangfangensis, Enterococcus faecium, Escherichia coli, Micrococcus sp., Salmonella typhimurium, Staphylococcus aureus, Streptococcus sp., Vibrio alginoliticus, V. proteolyticus, V. vulnificus) and one pathogenic yeast (Candida albicans). Among the Firmicutes, isolate P8, which is closely related to Bacillus pumilus, displayed the largest spectrum of growth inhibition of the pathogenic bacteria tested. The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

No MeSH data available.


Related in: MedlinePlus