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Antimicrobial Activities of Bacteria Associated with the Brown Alga Padina pavonica.

Ismail A, Ktari L, Ahmed M, Bolhuis H, Boudabbous A, Stal LJ, Cretoiu MS, El Bour M - Front Microbiol (2016)

Bottom Line: Eighteen isolates were obtained in pure culture and tested for antimicrobial activities.Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates).The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Marine Sciences and Technologies Salammb├┤, Tunisia.

ABSTRACT
Macroalgae belonging to the genus Padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. Hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by secondary metabolite producing epiphytic bacteria. Here we report antibacterial activities of epiphytic bacteria isolated from Padina pavonica (Peacocks tail) located on northern coast of Tunisia. Eighteen isolates were obtained in pure culture and tested for antimicrobial activities. Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates). The antimicrobial activity was assessed as inhibition of growth of 12 species of pathogenic bacteria (Aeromonas salmonicida, A. hydrophila, Enterobacter xiangfangensis, Enterococcus faecium, Escherichia coli, Micrococcus sp., Salmonella typhimurium, Staphylococcus aureus, Streptococcus sp., Vibrio alginoliticus, V. proteolyticus, V. vulnificus) and one pathogenic yeast (Candida albicans). Among the Firmicutes, isolate P8, which is closely related to Bacillus pumilus, displayed the largest spectrum of growth inhibition of the pathogenic bacteria tested. The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

No MeSH data available.


Related in: MedlinePlus

Plates testing antimicrobial activity of strain P8 isolated from P. pavonica by using the drop method and the overlay assay against A. salmonicida and Staphylococcus aureus.
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Figure 2: Plates testing antimicrobial activity of strain P8 isolated from P. pavonica by using the drop method and the overlay assay against A. salmonicida and Staphylococcus aureus.

Mentions: Inhibition zones depended on the method applied. Generally larger inhibition zones were observed when using the overlay assays compared to the drop assay (Table 3). The strongest effect was recorded for isolate P8 against S. aureus (inhibition zones varied from 20 to 45 mm; Figure 2). On the basis of its strong activity, isolate P8 was selected to test its antagonistic activity against nine Vibrio species, which are well known marine (fish, shellfish) pathogens. The overlay method was chosen for this test, showing a higher effectiveness when compared to the drop method. As shown in Table 4, two strains of Vibrio species tested were inhibited (V. proteolyticus and V. vulnificus) and furthermore E. xiangfangensis and E. faecium were inhibited as well.


Antimicrobial Activities of Bacteria Associated with the Brown Alga Padina pavonica.

Ismail A, Ktari L, Ahmed M, Bolhuis H, Boudabbous A, Stal LJ, Cretoiu MS, El Bour M - Front Microbiol (2016)

Plates testing antimicrobial activity of strain P8 isolated from P. pavonica by using the drop method and the overlay assay against A. salmonicida and Staphylococcus aureus.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940378&req=5

Figure 2: Plates testing antimicrobial activity of strain P8 isolated from P. pavonica by using the drop method and the overlay assay against A. salmonicida and Staphylococcus aureus.
Mentions: Inhibition zones depended on the method applied. Generally larger inhibition zones were observed when using the overlay assays compared to the drop assay (Table 3). The strongest effect was recorded for isolate P8 against S. aureus (inhibition zones varied from 20 to 45 mm; Figure 2). On the basis of its strong activity, isolate P8 was selected to test its antagonistic activity against nine Vibrio species, which are well known marine (fish, shellfish) pathogens. The overlay method was chosen for this test, showing a higher effectiveness when compared to the drop method. As shown in Table 4, two strains of Vibrio species tested were inhibited (V. proteolyticus and V. vulnificus) and furthermore E. xiangfangensis and E. faecium were inhibited as well.

Bottom Line: Eighteen isolates were obtained in pure culture and tested for antimicrobial activities.Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates).The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Marine Sciences and Technologies Salammb├┤, Tunisia.

ABSTRACT
Macroalgae belonging to the genus Padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. Hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by secondary metabolite producing epiphytic bacteria. Here we report antibacterial activities of epiphytic bacteria isolated from Padina pavonica (Peacocks tail) located on northern coast of Tunisia. Eighteen isolates were obtained in pure culture and tested for antimicrobial activities. Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates). The antimicrobial activity was assessed as inhibition of growth of 12 species of pathogenic bacteria (Aeromonas salmonicida, A. hydrophila, Enterobacter xiangfangensis, Enterococcus faecium, Escherichia coli, Micrococcus sp., Salmonella typhimurium, Staphylococcus aureus, Streptococcus sp., Vibrio alginoliticus, V. proteolyticus, V. vulnificus) and one pathogenic yeast (Candida albicans). Among the Firmicutes, isolate P8, which is closely related to Bacillus pumilus, displayed the largest spectrum of growth inhibition of the pathogenic bacteria tested. The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

No MeSH data available.


Related in: MedlinePlus