Limits...
Antimicrobial Activities of Bacteria Associated with the Brown Alga Padina pavonica.

Ismail A, Ktari L, Ahmed M, Bolhuis H, Boudabbous A, Stal LJ, Cretoiu MS, El Bour M - Front Microbiol (2016)

Bottom Line: Eighteen isolates were obtained in pure culture and tested for antimicrobial activities.Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates).The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Marine Sciences and Technologies Salammb├┤, Tunisia.

ABSTRACT
Macroalgae belonging to the genus Padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. Hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by secondary metabolite producing epiphytic bacteria. Here we report antibacterial activities of epiphytic bacteria isolated from Padina pavonica (Peacocks tail) located on northern coast of Tunisia. Eighteen isolates were obtained in pure culture and tested for antimicrobial activities. Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates). The antimicrobial activity was assessed as inhibition of growth of 12 species of pathogenic bacteria (Aeromonas salmonicida, A. hydrophila, Enterobacter xiangfangensis, Enterococcus faecium, Escherichia coli, Micrococcus sp., Salmonella typhimurium, Staphylococcus aureus, Streptococcus sp., Vibrio alginoliticus, V. proteolyticus, V. vulnificus) and one pathogenic yeast (Candida albicans). Among the Firmicutes, isolate P8, which is closely related to Bacillus pumilus, displayed the largest spectrum of growth inhibition of the pathogenic bacteria tested. The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

No MeSH data available.


Related in: MedlinePlus

Neighbor-joining tree based on 16S rRNA gene sequences derived from P. pavonica isolates and top five blast best hit sequences identified via GenBank search. (A) P9, P11, and P20 isolates and Pseudomonas genus (B) P8 isolate and Bacillus genus (C) P12 isolate and Pseudoalteromonas genus.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4940378&req=5

Figure 1: Neighbor-joining tree based on 16S rRNA gene sequences derived from P. pavonica isolates and top five blast best hit sequences identified via GenBank search. (A) P9, P11, and P20 isolates and Pseudomonas genus (B) P8 isolate and Bacillus genus (C) P12 isolate and Pseudoalteromonas genus.

Mentions: The 18 isolates were tested for their antimicrobial activities using two different methods and a number of selected pathogens. Five isolates: P8, P9, P11, P12, and P20 showed antibacterial activities against a broad range of pathogens (Table 3). Phylogenetic analysis of the 16S rRNA gene sequences of these active isolates was performed using the Neighbor Joining method (Figure 1).


Antimicrobial Activities of Bacteria Associated with the Brown Alga Padina pavonica.

Ismail A, Ktari L, Ahmed M, Bolhuis H, Boudabbous A, Stal LJ, Cretoiu MS, El Bour M - Front Microbiol (2016)

Neighbor-joining tree based on 16S rRNA gene sequences derived from P. pavonica isolates and top five blast best hit sequences identified via GenBank search. (A) P9, P11, and P20 isolates and Pseudomonas genus (B) P8 isolate and Bacillus genus (C) P12 isolate and Pseudoalteromonas genus.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940378&req=5

Figure 1: Neighbor-joining tree based on 16S rRNA gene sequences derived from P. pavonica isolates and top five blast best hit sequences identified via GenBank search. (A) P9, P11, and P20 isolates and Pseudomonas genus (B) P8 isolate and Bacillus genus (C) P12 isolate and Pseudoalteromonas genus.
Mentions: The 18 isolates were tested for their antimicrobial activities using two different methods and a number of selected pathogens. Five isolates: P8, P9, P11, P12, and P20 showed antibacterial activities against a broad range of pathogens (Table 3). Phylogenetic analysis of the 16S rRNA gene sequences of these active isolates was performed using the Neighbor Joining method (Figure 1).

Bottom Line: Eighteen isolates were obtained in pure culture and tested for antimicrobial activities.Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates).The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Marine Sciences and Technologies Salammb├┤, Tunisia.

ABSTRACT
Macroalgae belonging to the genus Padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. Hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by secondary metabolite producing epiphytic bacteria. Here we report antibacterial activities of epiphytic bacteria isolated from Padina pavonica (Peacocks tail) located on northern coast of Tunisia. Eighteen isolates were obtained in pure culture and tested for antimicrobial activities. Based on the 16S rRNA gene sequences the isolates were closely related to Proteobacteria (12 isolates; 2 Alpha- and 10 Gammaproteobacteria), Firmicutes (4 isolates) and Actinobacteria (2 isolates). The antimicrobial activity was assessed as inhibition of growth of 12 species of pathogenic bacteria (Aeromonas salmonicida, A. hydrophila, Enterobacter xiangfangensis, Enterococcus faecium, Escherichia coli, Micrococcus sp., Salmonella typhimurium, Staphylococcus aureus, Streptococcus sp., Vibrio alginoliticus, V. proteolyticus, V. vulnificus) and one pathogenic yeast (Candida albicans). Among the Firmicutes, isolate P8, which is closely related to Bacillus pumilus, displayed the largest spectrum of growth inhibition of the pathogenic bacteria tested. The results emphasize the potential use of P. pavonica associated antagonistic bacteria as producers of novel antibacterial compounds.

No MeSH data available.


Related in: MedlinePlus