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Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities.

Erban T, Klimov PB, Smrz J, Phillips TW, Nesvorna M, Kopecky J, Hubert J - Front Microbiol (2016)

Bottom Line: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations).Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

View Article: PubMed Central - PubMed

Affiliation: Biologically Active Substances in Crop Protection, Crop Research Institute Prague, Czech Republic.

ABSTRACT

Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed population of T. putrescentiae and T. fanetzhangorum collected from different habitats.

Material: The bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes.

Results: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacterium. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.

Conclusion: The presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea-like in the eggs of T. putrescentiae indicates mother to offspring (vertical) transmission. RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

No MeSH data available.


Related in: MedlinePlus

(A) The proportion of guanine waste product granules in T. putrescentiae; (B) comparison of sex ratios, male/(male+female) in examined populations of T. putrescentiae. Statistically significant differences (α = 0.05) in multiple comparisons are indicated by different letters. Abbreviations for the T. putrescentiae populations are listed in Table 1. (0) no guanine granules, (1) low number, granules fill less than 25% of the hysterosoma, (2) intermediate, granules fill between 25 and 50% of the hysterosoma, and (3) massive, granules fill more than 50% of the hysterosoma.
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Figure 10: (A) The proportion of guanine waste product granules in T. putrescentiae; (B) comparison of sex ratios, male/(male+female) in examined populations of T. putrescentiae. Statistically significant differences (α = 0.05) in multiple comparisons are indicated by different letters. Abbreviations for the T. putrescentiae populations are listed in Table 1. (0) no guanine granules, (1) low number, granules fill less than 25% of the hysterosoma, (2) intermediate, granules fill between 25 and 50% of the hysterosoma, and (3) massive, granules fill more than 50% of the hysterosoma.

Mentions: The guanin granulae filled hysterosoma of mites with different intensity (Figure 9). The populations significantly differed (Chi-square = 119.37, P < 0.001) in the guanine contents in their hysterosoma (Figure 10A). The differences were between Phillips population with prevailing low guanine contents and the rest of populations with high guanine contents (Figure 9).


Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities.

Erban T, Klimov PB, Smrz J, Phillips TW, Nesvorna M, Kopecky J, Hubert J - Front Microbiol (2016)

(A) The proportion of guanine waste product granules in T. putrescentiae; (B) comparison of sex ratios, male/(male+female) in examined populations of T. putrescentiae. Statistically significant differences (α = 0.05) in multiple comparisons are indicated by different letters. Abbreviations for the T. putrescentiae populations are listed in Table 1. (0) no guanine granules, (1) low number, granules fill less than 25% of the hysterosoma, (2) intermediate, granules fill between 25 and 50% of the hysterosoma, and (3) massive, granules fill more than 50% of the hysterosoma.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940368&req=5

Figure 10: (A) The proportion of guanine waste product granules in T. putrescentiae; (B) comparison of sex ratios, male/(male+female) in examined populations of T. putrescentiae. Statistically significant differences (α = 0.05) in multiple comparisons are indicated by different letters. Abbreviations for the T. putrescentiae populations are listed in Table 1. (0) no guanine granules, (1) low number, granules fill less than 25% of the hysterosoma, (2) intermediate, granules fill between 25 and 50% of the hysterosoma, and (3) massive, granules fill more than 50% of the hysterosoma.
Mentions: The guanin granulae filled hysterosoma of mites with different intensity (Figure 9). The populations significantly differed (Chi-square = 119.37, P < 0.001) in the guanine contents in their hysterosoma (Figure 10A). The differences were between Phillips population with prevailing low guanine contents and the rest of populations with high guanine contents (Figure 9).

Bottom Line: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations).Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

View Article: PubMed Central - PubMed

Affiliation: Biologically Active Substances in Crop Protection, Crop Research Institute Prague, Czech Republic.

ABSTRACT

Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed population of T. putrescentiae and T. fanetzhangorum collected from different habitats.

Material: The bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes.

Results: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacterium. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.

Conclusion: The presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea-like in the eggs of T. putrescentiae indicates mother to offspring (vertical) transmission. RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

No MeSH data available.


Related in: MedlinePlus