Limits...
Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities.

Erban T, Klimov PB, Smrz J, Phillips TW, Nesvorna M, Kopecky J, Hubert J - Front Microbiol (2016)

Bottom Line: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations).Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

View Article: PubMed Central - PubMed

Affiliation: Biologically Active Substances in Crop Protection, Crop Research Institute Prague, Czech Republic.

ABSTRACT

Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed population of T. putrescentiae and T. fanetzhangorum collected from different habitats.

Material: The bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes.

Results: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacterium. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.

Conclusion: The presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea-like in the eggs of T. putrescentiae indicates mother to offspring (vertical) transmission. RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

No MeSH data available.


Related in: MedlinePlus

Histological sections of T. putrescentiae: (A) Total view of T. putrescentiae with bacteriocyte (arrows); (B) details of the previous image; the arrows point to guanine crystals near the post-colon; (C) sagittal section of the mite body with bacteriocyte (arrows); (E) details of the previous image with spherical bacteria; (F) localization of bacteriocytes with spherical bacteria, (F) details of the bacteriocyte with rod-shaped bacteria. Staining: (A,B) Mann Dominici, (C,D) Ziehl–Neelsen, (E,F) Masson’s triple stain; Scales. (A,C) 100 μm, (D–F) 25 μm. ca, caecum; fb, food bolus; myc, bacteriocyte; pc, post-colon; sg, synganglion.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4940368&req=5

Figure 8: Histological sections of T. putrescentiae: (A) Total view of T. putrescentiae with bacteriocyte (arrows); (B) details of the previous image; the arrows point to guanine crystals near the post-colon; (C) sagittal section of the mite body with bacteriocyte (arrows); (E) details of the previous image with spherical bacteria; (F) localization of bacteriocytes with spherical bacteria, (F) details of the bacteriocyte with rod-shaped bacteria. Staining: (A,B) Mann Dominici, (C,D) Ziehl–Neelsen, (E,F) Masson’s triple stain; Scales. (A,C) 100 μm, (D–F) 25 μm. ca, caecum; fb, food bolus; myc, bacteriocyte; pc, post-colon; sg, synganglion.

Mentions: The bacteriocytes (Figure 8A) were found in some adults in all observed populations. The bacteria were localized in fat tissues and were of various sizes covering up to one third of the histological sections (Figure 8C). The bacteria in the bacteriocytes were formed by spherical (Figures 8B,D,E) particles with different staining compared to the rest of tissues; we identified these particles as bacteria. In addition, rod-shaped particles were found in the bacteriocytes in the laboratory population of T. putrescentiae (Figure 8F).


Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities.

Erban T, Klimov PB, Smrz J, Phillips TW, Nesvorna M, Kopecky J, Hubert J - Front Microbiol (2016)

Histological sections of T. putrescentiae: (A) Total view of T. putrescentiae with bacteriocyte (arrows); (B) details of the previous image; the arrows point to guanine crystals near the post-colon; (C) sagittal section of the mite body with bacteriocyte (arrows); (E) details of the previous image with spherical bacteria; (F) localization of bacteriocytes with spherical bacteria, (F) details of the bacteriocyte with rod-shaped bacteria. Staining: (A,B) Mann Dominici, (C,D) Ziehl–Neelsen, (E,F) Masson’s triple stain; Scales. (A,C) 100 μm, (D–F) 25 μm. ca, caecum; fb, food bolus; myc, bacteriocyte; pc, post-colon; sg, synganglion.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940368&req=5

Figure 8: Histological sections of T. putrescentiae: (A) Total view of T. putrescentiae with bacteriocyte (arrows); (B) details of the previous image; the arrows point to guanine crystals near the post-colon; (C) sagittal section of the mite body with bacteriocyte (arrows); (E) details of the previous image with spherical bacteria; (F) localization of bacteriocytes with spherical bacteria, (F) details of the bacteriocyte with rod-shaped bacteria. Staining: (A,B) Mann Dominici, (C,D) Ziehl–Neelsen, (E,F) Masson’s triple stain; Scales. (A,C) 100 μm, (D–F) 25 μm. ca, caecum; fb, food bolus; myc, bacteriocyte; pc, post-colon; sg, synganglion.
Mentions: The bacteriocytes (Figure 8A) were found in some adults in all observed populations. The bacteria were localized in fat tissues and were of various sizes covering up to one third of the histological sections (Figure 8C). The bacteria in the bacteriocytes were formed by spherical (Figures 8B,D,E) particles with different staining compared to the rest of tissues; we identified these particles as bacteria. In addition, rod-shaped particles were found in the bacteriocytes in the laboratory population of T. putrescentiae (Figure 8F).

Bottom Line: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations).Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

View Article: PubMed Central - PubMed

Affiliation: Biologically Active Substances in Crop Protection, Crop Research Institute Prague, Czech Republic.

ABSTRACT

Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed population of T. putrescentiae and T. fanetzhangorum collected from different habitats.

Material: The bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes.

Results: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacterium. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.

Conclusion: The presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea-like in the eggs of T. putrescentiae indicates mother to offspring (vertical) transmission. RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

No MeSH data available.


Related in: MedlinePlus